Evanescent wave absorption based polyaniline cladding modified fiber optic intrinsic biosensor for glucose sensing application

Pahurkar, V. G.; Tamgadge, Y. S.; Gambhire, A.B.; Muley, G.G.

doi:10.1016/j.measurement.2014.10.029

Cited by 31 publications

(15 citation statements)

References 63 publications

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“…The sensing element was rinsed with phosphate buffer solution to generate a hydrophilic surface. The GOx was immobilized over modified PANI‐OA composite matrix surface though cross‐linking via 1% glutaraldehyde solution using layer‐by‐layer technique . After this, it was allowed to dry for 30 min and washed 2–3 times with phosphate buffer to leach out the loosely bound GOx molecules.…”

Section: Methodsmentioning

confidence: 99%

Cladding Modified with PANI‐Oxalic Acid Composite Glucose Oxidase Immobilized Fiber Optic Intrinsic Glucose Biosensor

Pahurkar

Dagdale

Tamgadge³

et al. 2016

Macromolecular Symposia

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Summary: Polyaniline (PANI) and its composites have drawn considerable interest because of their economical importance, good environmental stability and satisfactory conductivity among the conductive polymers. Polymers are playing an increasingly important role in the development of biosensors. The use of conducting polymer composites for the construction of biosensor is increasing nowadays for the immobilization of biomolecules as it enhances the activity and performance of the sensor. In the present investigation, we have used PANI-oxalic acid (OA) for the cladding modification to prepare fiber optic intrinsic glucose biosensor (FOIGB) for detection of glucose. PANI-OA composite have shown good binding toward enzyme-glucose oxidase (GOx) and high biological activity that results in enhanced sensing characteristics. This investigation highlights approach to synthesize PANI-OA composite and its applications in cladding modified FOIB technology. The characterizations were made using X-ray diffraction and scanning electron microscopy (SEM) techniques. The results revealed the formation of homogeneous, PANI-OA film on sensing element. The sensing response of FOIGB toward glucose was measured by monitoring variation in power with varying glucose concentration.

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Section: Methodsmentioning

confidence: 99%

Cladding Modified with PANI‐Oxalic Acid Composite Glucose Oxidase Immobilized Fiber Optic Intrinsic Glucose Biosensor

Pahurkar

Dagdale

Tamgadge³

et al. 2016

Macromolecular Symposia

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“…As reported earlier, PANI‐ a conducting polymer is an intelligent material for cladding modification in optical fiber sensor among the others . It has nice accommodating surface, good biocompatibility, stability, efficient polymerization at neutral pH, easy to synthesize, and able to increase sensitivity of biosensor . Moreover, to increase the performance of the biosensor other organic/inorganic materials can be introduced into the PANI matrix.…”

Section: Introductionmentioning

confidence: 95%

Performance of Cladding Modified PANI‐ZnO Nanocomposite Glucose Oxidase Immobilized Fiber Optic Intrinsic Glucose Biosensor

Pahurkar

Harde²,

Nagale

et al. 2016

Macromolecular Symposia

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Summary:The cheapness, easy availability of raw materials, easy synthesis, good environmental stability, good electrical and optical properties, simple doping and de-doping chemistry makes the place of polymers very important in sensing applications. Besides all the conducting polymers, polyaniline (PANI) has found comfortable and suitable for the immobilization of biomolecules. In the present study, we have fabricated PANI-ZnO nanocomposite by chemical polymerization route and used to modify cladding of an optical fiber for immobilization of enzyme glucose oxidase (GOx) on it with the help of cross-linking technique for the development of cladding modified fiber optic intrinsic glucose biosensor (FOIGB). The properties of deposited PANI-ZnO nanocomposite have been studied. The sensitivity of prepared sensor in the form of power and modal power distribution (MPD) has also been reported.

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“…There have three immobilization of pH indicator method; adsorption, entrapment and covalent binding but entrapment method become frequently used because it is easy, reliable but slow indicator leaching [5,[15][16][17][18]. Some optical sensors have been reported optical signal are based on reflectance, absorbance and fluorescence [15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…Some optical sensors have been reported optical signal are based on reflectance, absorbance and fluorescence [15][16][17][18][19][20][21]. The optical pH sensor requires some indicator sensitive to pH that able to convert the analyte concentration into an optical signal.…”

Section: Introductionmentioning

confidence: 99%

Optical pH sensor based on polyaniline sol-gel film immobilized with bromothymol blue and phenol red

Othman¹,

Hanim

Noor

et al. 2016

AIP Conference Proceedings

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Evanescent wave absorption based polyaniline cladding modified fiber optic intrinsic biosensor for glucose sensing application

Cited by 31 publications

References 63 publications

Cladding Modified with PANI‐Oxalic Acid Composite Glucose Oxidase Immobilized Fiber Optic Intrinsic Glucose Biosensor

Cladding Modified with PANI‐Oxalic Acid Composite Glucose Oxidase Immobilized Fiber Optic Intrinsic Glucose Biosensor

Performance of Cladding Modified PANI‐ZnO Nanocomposite Glucose Oxidase Immobilized Fiber Optic Intrinsic Glucose Biosensor

Optical pH sensor based on polyaniline sol-gel film immobilized with bromothymol blue and phenol red

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