Evening primrose seed extract rich in polyphenols modulates the invasiveness of colon cancer cells by regulating the TYMS expression

Włodarczyk, Jakub; Chmielewska-Kassassir, Małgorzata; Fichna, Jakub; Woźniak, Lucyna A.; Sobierajska, Katarzyna

doi:10.1039/d2fo01737g

Cited by 4 publications

(11 citation statements)

References 61 publications

(118 reference statements)

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“…The CACRC mouse model was established with AOM injection, followed by three cycles of proinflammatory DSS oral administration. In this model, carcinogenesis and tumor development in the distal colon were reported previously [ 19 , 20 ].…”

Section: Resultsmentioning

confidence: 97%

“…Colitis-associated colorectal cancer was induced by a single intraperitoneal administration of azoxymethane (AOM) at the dose of 10 mg/kg as described earlier [ 19 , 20 ]. One week later, the autoclaved drinking water was replaced by a 1.5% (w/v) dextran sodium sulfate (DSS) solution.…”

Section: Methodsmentioning

confidence: 99%

“…A total macroscopic damage score was calculated for each animal as described previously [ 19 , 20 ]. The macroscopic scoring was based on five parameters: diarrhea (normal = 0; soft = 1; liquid = 2), presence of bleeding (no = 0; yes = 2), edema (no = 0; mild = 1; intense = 2), erythema (no = 0; mild = 1; intense = 2), and adhesions (no adhesion = 0; troublesome dissection = 1; visible adhesions = 2).…”

Section: Methodsmentioning

confidence: 99%

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New gold(III) complexes TGS 121, 404, and 702 show anti-tumor activity in colitis-induced colorectal cancer: an in vitro and in vivo study

Włodarczyk,

Krajewska,

Talar

et al. 2023

Pharmacol. Rep

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Background Chronic inflammation in the course of inflammatory bowel disease may result in colon cancer, or colitis-associated colorectal cancer (CACRC). It is well established that CACRC is associated with oxidative stress and secretion of multiple pro-inflammatory cytokines, e.g. tumor necrosis factor-α. Recently, we proved that the administration of gold(III) complexes resulted in the alleviation of acute colitis in mice. The aim of the current study was to assess the antitumor effect of a novel series of gold(III) complexes: TGS 121, 404, 512, 701, 702, and 703. Materials Analyzed gold(III) complexes were screened in the in vitro studies using colorectal cancer and normal colon epithelium cell lines, SW480, HT-29, and CCD 841 CoN, and in vivo, in the CACRC mouse model. Results Of all tested complexes, TGS 121, 404, and 702 exhibited the strongest anti-tumor effect in in vitro viability assay of colon cancer cell lines and in in vivo CACRC model, in which these complexes decreased the total number of colonic tumors and macroscopic score. We also evidenced that the mechanism of action was linked to the enzymatic antioxidant system and inflammatory cytokines. Conclusions TGS 121, 404, and 702 present anti-tumor potential and are an attractive therapeutic option for colorectal cancer.

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Section: Resultsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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New gold(III) complexes TGS 121, 404, and 702 show anti-tumor activity in colitis-induced colorectal cancer: an in vitro and in vivo study

Włodarczyk,

Krajewska,

Talar

et al. 2023

Pharmacol. Rep

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“…Polyphenols are an important group of plant metabolites as their consumption reduces the risk of cancer development [6]. The beneficial antitumor effect of these phytochemicals, both as individual compounds and their combinations, is reflected in the elevation of cancer cell death via the stimulation of apoptotic signaling and the inhibition of proliferating signals [7][8][9]. A modulatory effect on the EMT process is also attributed to their role [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…A modulatory effect on the EMT process is also attributed to their role [10,11]. Evening primrose extracts (EPE), rich in polyphenols, have been isolated from postindustrial defatted Oenothera paradoxa seeds and revealed potential anti-cancer properties [9,[12][13][14]. It is known that EPE constituents might inhibit the metastatic abilities of cancer cells by downregulating metalloproteinase expression and secretion [15,16].…”

Section: Introductionmentioning

confidence: 99%

Evening Primrose Extract Modulates TYMS Expression via SP1 Transcription Factor in Malignant Pleural Mesothelioma

Chmielewska-Kassassir,

Sobierajska,

Ciszewski

et al. 2023

Cancers

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Purpose: To determine the mechanism of EPE in downregulating TYMS in MPM cancer. Methods: The TYMS mRNA expression with epithelial-to-mesenchymal transition biomarkers and nuclear factor SP1 was assessed using the GEO database in a data set of MPM patients (GSE51024). Invasive MPM cell lines were in vitro models for the investigation of TYMS expression after EPE treatment. The tyms promoter SP1 binding sequences were determined using Genomatix v 3.4 software Electrophoretic mobility shift and dual-luciferase reporter assays revealed specific SP1 motifs in the interaction of EPE and reference compounds. Chromatin immunoprecipitation and Re-ChIP were used for the co-occupancy study. Results: In MPM patients, a positive correlation of overexpressed TYMS with mesenchymal TWIST1, FN1 and N-cadherin was observed. EPE and its major components, gallic and ellagic acid (GA and EA, respectively), downregulated TYMS in invasive MPM cells by interacting with particular SP1 motifs on the tyms promoter. The luciferase constructs confirmed the occupation of two SP1 regulatory regions critical for the promotion of TYMS expression. Both EPE and reference standards influenced SP1 translocation into the nucleus. Conclusion: EPE components reduced TYMS expression by occupation of SP1 motifs on the tyms promoter and reversed the EMT phenotype of invasive MPM cells. Further in-depth analysis of the molecular docking of polyphenol compounds with SP1 regulatory motifs is required.

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Whole grain metabolite 3,5-dihydroxybenzoic acid is a beneficial nutritional molecule with the feature of a double-edged sword in human health: a critical review and dietary considerations

Wagner

Sobierajska

Pułaski

et al. 2023

Critical Reviews in Food Science and Nutrition

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Evening primrose seed extract rich in polyphenols modulates the invasiveness of colon cancer cells by regulating the TYMS expression

Abstract: Natural polyphenols are plant metabolites exhibiting a broad range of biological activities.

Cited by 4 publications

References 61 publications

New gold(III) complexes TGS 121, 404, and 702 show anti-tumor activity in colitis-induced colorectal cancer: an in vitro and in vivo study

New gold(III) complexes TGS 121, 404, and 702 show anti-tumor activity in colitis-induced colorectal cancer: an in vitro and in vivo study

Evening Primrose Extract Modulates TYMS Expression via SP1 Transcription Factor in Malignant Pleural Mesothelioma

Whole grain metabolite 3,5-dihydroxybenzoic acid is a beneficial nutritional molecule with the feature of a double-edged sword in human health: a critical review and dietary considerations

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