Evidence for a functional glycogen metabolism in mature mammalian spermatozoa

Ballester, Joan; Fernández‐Novell, Josep M.; Rutllant, J.; Garcı́a-Rocha, Mar; Palomo, M.J.; Mogas, T.; Peña, Alejandro; Rigau, Teresa; Guinovart, Joan J.; Rodríguez‐Gil, Joan E.

doi:10.1002/(sici)1098-2795(200006)56:2<207::aid-mrd12>3.0.co;2-4

Cited by 64 publications

(50 citation statements)

References 20 publications

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“…6, modified from Mathews et al) [33,47,48]. Several studies have also shown that glycogen is present in dog sperm, suggesting that gluconeogenesis is important to maintain motility and to achieve in vitro capacitation [49,50]. Considering that glycogen is stored in human sperm, and sperm from numerous species can remain motile in glucose-free media, it is highly likely that human sperm might be capable of gluconeogenesis [51,52].…”

Section: Discussionmentioning

confidence: 99%

Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker

Tasi

Chao

Chung

et al. 2013

J Assist Reprod Genet

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Purpose Asthenozoospermia is a major cause of male infertility. However, the molecular mechanisms underlying spermmotility defects remain largely unknown in the majority of cases. In our previous study, we applied a proteomic approach to identify unknown proteins that were downregulated in spermatozoa with low motility compared to spermatozoa with good motility. Several sperm motility-related proteins have been identified. In this study, 3-hydroxyisobutyrate dehydrogenase (HIBADH), one of the proteins identified using the proteomic tools, is further characterized.Methods Reverse-transcription polymerase chain reactions (RT-PCR), western blotting, and immunofluorescence assays (IFA) were preformed to investigate the expression pattern. The enzymatic activity of HIBADH was evaluated in sperm with good (>50 %), moderate (< 50 %) and lower motility (< 20 %). Results Using RT-PCR, we found that transcripts of HIBADH are enriched in the cerebellum, heart, skeletal muscle, uterus, placenta, and testes of male humans. In western blotting, it is expressed in the placenta, testes, and spermatozoa. During spermiogenesis, HIBADH is located at Capsule HIBADH is involved in the mitochondrial function of spermatozoa and maintains sperm motility. It may serve as a spermmotility marker.Pao-Lin Kuo and Ying-Hung Lin contributed equally to co-corresponding author. Assist Reprod Genet (2013) 30:505-512 DOI 10.1007 the mid-piece (a specialized development from the mitochondria) of elongating, elongated, and mature sperm. The enzymatic activity of HIBADH in sperm with moderate and lower motility were significantly reduced compared with good motility (P<0.0001 and P<0.05, respectively). Conclusions Our study indicated that HIBADH is involved in the mitochondrial function of spermatozoa, and maintains sperm motility. It may serve as a sperm-motility marker. Electronic supplementary material

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Section: Discussionmentioning

confidence: 99%

Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker

Tasi

Chao

Chung

et al. 2013

J Assist Reprod Genet

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show abstract

“…This re-serve would play an important role in the maintenance of "in vivo" sperm survival. In fact, as discussed above, the existence of a fully functional glycogen metabolism has been demonstrated in sperm from species like dog, boar, horse and ram [5]. Remarkably, dog sperm shows the most active glycogen metabolism of all of the studied species, in this way accumulating the maximal recorded intracellular levels [5].…”

Section: Metabolic Phenotypes: a Results Of The Separate Evolutionary mentioning

confidence: 67%

“…In fact, as discussed above, the existence of a fully functional glycogen metabolism has been demonstrated in sperm from species like dog, boar, horse and ram [5]. Remarkably, dog sperm shows the most active glycogen metabolism of all of the studied species, in this way accumulating the maximal recorded intracellular levels [5]. As described above, this glycogen plays an important role in the achievement of feasible "in vitro" capacitation [1,2], reinforcing thus the importance of this anabolic pathway in dog.…”

Section: Metabolic Phenotypes: a Results Of The Separate Evolutionary mentioning

confidence: 79%

“…One of the most studied sources is glycogen. The presence of a functional glycogen metabolism has been demonstrated in several species, such as dog, boar, horse, ram and bonnet monkey [5], although no glycogen was found in other species like mice and rat [3]. The primary role of these internal energy sources would be, logically, the maintenance of a limited energy reservoir, although this could not be a universal function.…”

Section: Energy Sources For Mammalian Spermatozoamentioning

confidence: 99%

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Energy Management of Mature Mammalian Spermatozoa

Rodríguez‐Gil¹

2013

Success in Artificial Insemination - Quality of Semen and Diagnostics Employed

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“…In case of polymeric metabolites, such as glycogen, samples must be pretreated in order to degrade the polymer into their monomers by using the appropriate degrading enzymes. For instance, determination of intracellular sperm glycogen levels requires a previous incubation of -amyloglucosydase, in order to release all of the glucose contained in the polymer (Ballester et al, 2000;Palomo et al, 2003). Then, this released glucose is determined through a standard, spectrophotometric technique (Ballester et al, 2000;Palomo et al, 2003).…”

Section: Metabolic Analyses: Metabolite Levelsmentioning

confidence: 99%

Spermiomics: A New Term Describing the Global Survey of the Overall Sperm Function by the Combined Utilization of Immunocytochemistry, Metabolomics, Proteomics and Other Classical Analytical Techniques

Rodríguez‐Gil¹

2012

Applications of Immunocytochemistry

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Evidence for a functional glycogen metabolism in mature mammalian spermatozoa

Cited by 64 publications

References 20 publications

Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker

Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker

Energy Management of Mature Mammalian Spermatozoa

Spermiomics: A New Term Describing the Global Survey of the Overall Sperm Function by the Combined Utilization of Immunocytochemistry, Metabolomics, Proteomics and Other Classical Analytical Techniques

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