Evidence for altered cell‐cycle traverse of the non‐modal cells of the heteroploid MCa‐11 line

Dooley, William C.; Allison, David C.; Lin, Paul; Paul, Michael

doi:10.1111/j.1365-2184.1993.tb00330.x

Cited by 3 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…38 A subsequent study demonstrated that nonmodal cells were capable of continued cell proliferation but were delayed temporarily in the G 2 and/or the prophase-metaphase stages of the cell cycle relative to cells with a modal DNA content. 39 Our data support this hypothesis in that the informational content of the diploid or pseudo-diploid cells of the tumorigenic populations obviously differs from that of the diploid cells of the nontumorigenic founding population of WB-F344 cells. Chemical transformants of WB-F344 cells often contained a diploid or near-diploid DNA content by flow cytometry but exhibited chromosomal aneuploidy by karyotypic analysis.…”

Section: Discussionsupporting

confidence: 79%

Genomic fluidity is a necessary event preceding the acquisition of tumorigenicity during spontaneous neoplastic transformation of WB-F344 rat liver epithelial cells

et al. 1998

View full text Add to dashboard Cite

The genomic evolution of a cohort of WB-F344 rat liver epithelial cell lineages undergoing spontaneous neoplastic transformation was followed to define the mechanistic relationship between genomic instability and progression to the neoplastic phenotype. Eighteen independent populations of WB-F344 cells (initiated from a single diploidfounding population) were subjected to 12 cycles of selective growth at confluent cell density, and cellular DNA contents were measured after each selection cycle. Flow cytometry demonstrated significant gains in the amount of G 1 DNA after selection cycles 3, 6, and 7 in 44% (8 of 18), 89% (16 of 18), and 39% (7 of 18) of the cell populations, respectively. All populations subsequently lost DNA and returned to a diploid or pseudo-diploid DNA content within 1 to 2 selection cycles after the appearance of an increased DNA content. Additionally, appearance and subsequent disappearance of aneuploid or tetraploid subpopulations was observed in 11% (2 of 18) and 83% (15 of 18) of the experimental lineages, respectively. Although perturbations of G 1 DNA content were apparent as early as selection cycle 3, at least 8 cycles of selective growth were required for the acquisition of tumorigenicity. While the independent lineages demonstrated significant fluctuations in G 1 DNA content between selection cycles 3 and 8, the majority (11 of 13) of the populations contained a diploid or pseudodiploid DNA content at the time tumorigenicity was expressed. Genomic instability preceded the acquisition of tumorigenic potential in rat liver epithelial cells subjected to selective growth conditions of maintenance at confluence, and may be required for its expression. (HEPATOLOGY 1998;28:78-85.) DNA content analysis by flow cytometry is a useful prognostic indicator of neoplasia. In many solid tumors, a diploid DNA pattern is associated with better patient survival than an aneuploid DNA pattern, which often predicts poor patient survival. Flow cytometric analyses of the relationship between DNA ploidy pattern and patient prognosis in human hepatocellular carcinoma have produced conflicting results. A positive correlation has been reported between a diploid DNA pattern, small tumor size (Ͻ5 cm), and low tumor grade. [1][2][3][4][5] However, the significance of this correlation as it relates to patient prognosis is not clear. Tumor diploidy has been demonstrated to be positively correlated with long-term patient survival in some, [2][3][4]6,7 but not other studies. 1,5,[8][9][10] In fact, Ng et al. 11 found that a diploid DNA pattern was associated with poorer patient survival than was an aneuploid pattern in large hepatocellular carcinomas. A diploid DNA content in a tumor does not preclude the possibility that small chromosomal alterations such as balanced translocations, point mutations, and deletions are present in the tumorigenic cells. Such subtle lesions may not be detected by flow cytometry. Alternatively, the finding of diploidy in a tumor may not preclude that the tumorigenic cells underwent a ...

show abstract

Section: Discussionsupporting

confidence: 79%

Genomic fluidity is a necessary event preceding the acquisition of tumorigenicity during spontaneous neoplastic transformation of WB-F344 rat liver epithelial cells

et al. 1998

View full text Add to dashboard Cite

show abstract

“…Its presence has been correlated with a high risk of recurrences and metastases 56,57 . Aneuploid cells are able to complete mitosis regardless of chromosome number or DNA content although with a prolonged mitotic time 58 . From this point of view, the bad prognostic value of a high mitotic index could be because of a prolonged mitotic duration and not to an increase in the proportion of cells in mitosis.…”

Section: Discussionmentioning

confidence: 99%

“…56,57 Aneuploid cells are able to complete mitosis regardless of chromosome number or DNA content although with a prolonged mitotic time. 58 From this point of view, the bad prognostic value of a high mitotic index could be because of a prolonged mitotic duration and not to an increase in the proportion of cells in mitosis. As mitosis reflects a small proportion of the cell cycle, longer mitosis duration has little impact on the cell cycle time and therefore on MIB-1 staining.…”

Section: The Mib-1 Indexmentioning

confidence: 99%

Significance of cell kinetic parameters in the prognosis of malignant melanoma: a review

2007

View full text Add to dashboard Cite

Malignant melanoma has been extensively studied concerning methods of predicting progression and clinical outcome. The maximum tumor thickness as measured by Breslow's method is the cornerstone prognostic criterion, but despite this, evolution of the disease in some patients remains unpredictable, confirming that new reliable prognostic factors are awaited. Cell kinetic evaluation has been shown to be a useful tool for assessing the prognosis of breast and gastrointestinal cancer patients. Indeed, in these fields, the mitotic index and MIB-1 expression index, which are indirect estimates of the growth fraction of tumor cell population, are commonly shown to correlate with tumor grade and patient survival and presented as prognostic factors. In melanoma, results of cell kinetic investigations are conflicting: some studies have established a link between high proliferative activity and a bad prognosis, whereas other reports suggest the opposite. The aim of this review is to discuss these findings.

show abstract

Tissue Culture, Cell Growth, and Analysis

2001

View full text Add to dashboard Cite

Evidence for altered cell‐cycle traverse of the non‐modal cells of the heteroploid MCa‐11 line

Cited by 3 publications

References 14 publications

Genomic fluidity is a necessary event preceding the acquisition of tumorigenicity during spontaneous neoplastic transformation of WB-F344 rat liver epithelial cells

Genomic fluidity is a necessary event preceding the acquisition of tumorigenicity during spontaneous neoplastic transformation of WB-F344 rat liver epithelial cells

Significance of cell kinetic parameters in the prognosis of malignant melanoma: a review

Tissue Culture, Cell Growth, and Analysis

Contact Info

Product

Resources

About