Evidence for circulating hemocyte proliferation in the shrimp Penaeus japonicus

Sequeira, Teresa; Tavares, Delfina; Arala-Chaves, M.P.

doi:10.1016/0145-305x(96)00001-8

Cited by 128 publications

(80 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, an active hemocyte-producing HPT was detected after the recovery phase in infectious hypodermal and hematopoietic necrosis (IHHN) virus-infected shrimp and after fungal infection (Hose et al, 1984;Lightner et al, 1983). In a subsequent study in Penaeus japonicas, a similar rapid increase in newly released cells with ongoing DNA synthesis was detected after LPS injection and infection with Fusarium fungi (Sequeira et al, 1996). Not only bacterial or fungal infections affect the HPT; viral infections might also impact hemocyte synthesis.…”

Section: Reaction To Microbial Polysaccharides and Repeated Bleedingmentioning

confidence: 79%

Crustacean hematopoiesis

Söderhäll

2016

Developmental & Comparative Immunology

168

138

View full text Add to dashboard Cite

Crustacean hemocytes are important mediators of immune reactions, and the regulation of hemocyte homeostasis is of utmost importance for the health of these animals. This review discusses the current knowledge on the lineages, synthesis and differentiation of hemocytes in crustaceans. Hematopoietic tissues, their origins, and the regulation of hematopoiesis during molting, seasonal variation and infection are discussed. Furthermore, studies concerning the molecular regulation of hemocyte formation in crustaceans are also described, and the different lineages and their molecular markers are discussed and compared with several insect species. Signaling pathways and the regulation of hematopoiesis by transcription factors are typically conserved among these arthropods, whereas cytokines and growth factors are more variable and species specific. However, considering the great diversity among the crustaceans, one should be cautious in drawing general conclusions from studies of only a few species.2

show abstract

Section: Reaction To Microbial Polysaccharides and Repeated Bleedingmentioning

confidence: 79%

Crustacean hematopoiesis

Söderhäll

2016

Developmental & Comparative Immunology

168

138

View full text Add to dashboard Cite

show abstract

“…The flow cytometry approach employed in this study offers several advantages compared to microscopy, as it is possible to analyze individual cell types, and to sort and harvest a particular kind of cell within a short period of time. Recently, this approach has been used in several studies of shrimp hemocytes (Sequeira et al 1996, Lee et al 2001. Using a cell sorter, we have sorted and harvested each type of the 3 hemocytes for further study.…”

Section: Discussionmentioning

confidence: 99%

“…Hemolymph randomly pooled from 10 shrimp of each batch, on Days 1 to 5, was fixed in 10% NBF solution. The hemocytes were prefiltered through a 200 µm mesh gauze and suspended in shrimp salt solution (SSS, 450 mM NaCl, 10 mM KCl, 10 mM EDTA, 10 mM HEPES, pH 7.3, 850 mOsm kg -1 ; Rodriguez et al 1995, Sequeira et al 1996. Hemocytes were sorted based on their cellular granularity and cell sizes on a FACSCalibur sorter using the CellQuest program (Becton-Dickinson).…”

Section: Methodsmentioning

confidence: 99%

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

Wang¹,

Liu²,

Seah³

et al. 2002

Dis. Aquat. Org.

View full text Add to dashboard Cite

White spot syndrome virus (WSSV) was specifically detected by PCR in Penaeus merguiensis hemocytes, hemolymph and plasma. This suggested a close association between the shrimp hemolymph and the virus. Three types of hemocyte from shrimp were isolated using flow cytometry. Dynamic changes of the hemocyte subpopulations in P. merguiensis at different times after infection were observed, indicating that the WSSV infection selectively affected specific subpopulations. Immunofluorescence assay (IFA) and a Wright-Giemsa double staining study of hemocyte types further confirmed the cellular localization of the virus in the infected hemocytes. Electron microscopy revealed virus particles in both vacuoles and the nucleus of the semigranular cells (SGC), as well as in the vacuoles of the granular cells (GC). However, no virus could be detected in the hyaline cells (HC). Our results suggest that the virus infects 2 types of shrimp hemocytes -GCs and SGCs. The SGC type contains higher virus loads and exhibits faster infection rates, and is apparently more susceptible to WSSV infection.

show abstract

“…There is also evidence that expression of some immune-related proteins can be stimulated by yeast infections in shrimp. For example, Sequeira et al (1996) reported that proliferating haemocytes were significantly increased in Marsupenaeus japonicus after stimulation by p43, an immunosuppressive lymphocyte mitogenic protein produced by Candida albicans. A similar response resulted from infection with Fusarium spp.…”

Section: Nucleotide and Amino-acid Sequences Of C57mentioning

confidence: 99%

“…A similar response resulted from infection with Fusarium spp. (Sequeira et al 1996). It is also known that betaglucan from yeast can initiate the shrimp prophenoloxidase (ProPO) defense cascade (Sritunyalucksana & Soderhall 2000).…”

Section: Nucleotide and Amino-acid Sequences Of C57mentioning

confidence: 99%

Upregulation of actin-like gene expression in giant freshwater prawns Macrobrachium rosenbergii infected with Metschnikowia bicuspidata

Wang²,

Lien³

et al. 2005

Dis. Aquat. Org.

View full text Add to dashboard Cite

The giant freshwater prawn Macrobrachium rosenbergii is commercially cultured throughout the world including Taiwan. From 1992 to 1995, Taiwanese production decreased by approximately 50% due to disease. The yeast Metschnikowia bicuspidata is considered to be one of the major causes of white muscle disease, but the molecular mechanism of its pathogenesis is not known. Using RNA differential display (DD) with muscle and hepatopancreatic tissue, we identified a 324 nucleotide (nt) message specifically expressed by M. rosenbergii infected with M. bicuspidata but not in the controls. A ribonuclease protection assay (RPA) confirmed expression in both tissues. RPA data also revealed an additional 230 bp mRNA message that was not identified by DD. Using RNA ligase-mediated rapid amplification of 5' cDNA ends (5'-RACE), we successfully isolated a 1357 bp full-length gene (c57 ) that showed 92 and 87% sequence identity to the actin gene of the Kuruma shrimp Marsupenaeus japonicus (also called Penaeus japonicus) (GenBank accession number AB055975) and the beta-actin gene of the white shrimp Litopenaeus vannamei (also called Penaeus vannamei) (GenBank accession number AF300705), respectively. The deduced amino acid sequence of c57 showed 83% sequence similarity to M. japonicus and L. vannamei actin proteins. Based on this high homology, we suggest that upregulation of actin expression in the muscle and hepatopancreas is part of the shrimp response to M. bicuspidata infection. Increased expression may be related to repair of tissues damaged by yeast infection. KEY WORDS: Macrobrachium rosenbergii · Metschnikowia bicuspidata · ActinResale or republication not permitted without written consent of the publisher **The nucleotide sequence data reported in this paper has been submitted to GenBank and has been assigned accession number AY651918

show abstract

Evidence for circulating hemocyte proliferation in the shrimp Penaeus japonicus

Cited by 128 publications

References 9 publications

Crustacean hematopoiesis

Crustacean hematopoiesis

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

Upregulation of actin-like gene expression in giant freshwater prawns Macrobrachium rosenbergii infected with Metschnikowia bicuspidata

Contact Info

Product

Resources

About