Evidence for double-stranded RNA from polyhedral virus-like particles in Lentinus edodes (Berk.) Sing

Ushiyama, R.; Nakai, Y.; Ikegami, Machiko

doi:10.1016/0042-6822(77)90512-8

Cited by 23 publications

(4 citation statements)

References 10 publications

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“…Many of the Japanese commercial strains were also found to carry 11.1 and/or 12.1 kb elements. Because L. edodes is known to contain some double-stranded RNA viruses (Ushiyama et al, 1977), RNase and DNase digestion tests were perfomed on these six elements. They were found to be digested by DNase, but not by RNase (data not shown).…”

Section: Detection Of Plasmids Total Dnas Extracted From 90mentioning

confidence: 99%

Detection and distribution of six linear mitochondrial plasmids in the shiitake mushroom, Lentinula edodes

1998

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The presence of plasmids was surveyed in 90 wild isolates of Lentinula edodes collected from geographically different world regions. DNA plasmids of different sizes were found in about 80% of the isolates. The plasmids detected were of six kinds, designated as pLE1 (9.0 kb), pLE2 (11.1 kb, =pLLE1 described by other authors), pLE3A (9.8 kb), pLE3B (10.8 kb), pLE3C (12.1 kb), and pLE3D (12.3 kb). Hybridization analysis suggested that pLE1 and pLE2 were distinct plasmid types of different homology groups to each other, and the four other plasmids were variant types belonging to a third homology group. These plasmids had no homology with their host's and non-host's nuclear and mitochondrial genome DNAs. Restriction analysis and electron microscopy indicated that the plasmids are linear in form. Since all six plasmids were transmitted uniparentally in sexual crosses and were consistently associated with the DNA preparations from mitochondria fractionated from mycelia of representative isolates, they were suggested to be located in mitochondria, similar to many other known fungal DNA plasmids. Geographically, pLE1 and pLE2 were widely distributed in natural populations of L. edodes, while the remaining four plasmids were uniquely present in delimited natural populations.

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Section: Detection Of Plasmids Total Dnas Extracted From 90mentioning

confidence: 99%

Detection and distribution of six linear mitochondrial plasmids in the shiitake mushroom, Lentinula edodes

1998

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“…Moreover, all of the well characterised isometric viruses having a diameter within the range 25-50 nm from L. edodes were thought to contain dsRNA (Ushiyama, Nakai & Ikegami, 1977;Ushiyama, 1983). We are the first to describe VLPs with ssRNA from diseased Shiitake mushroom.…”

Section: Discussionmentioning

confidence: 99%

The detection of single‐stranded RNA in an isometric virus‐like particle from Shiitake mushroom [Lentinus edodes (Berk.) Sing.]

Xueren

Chen

Juying

et al. 1993

Annals of Applied Biology

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Isometric virus-like particles (VLPs) with diameter of approximately 34 nm containing ss-RNA were purified from abnormal mycelium of Shiitake mushroom, Lentinus edodes (Berk.) Sing. SDS-polyacrylamide gel electrophoresis demonstrated that the virions contain a single capsid polypeptide with molecular weight of about 22 000 daltons. The nucleic acid extract from purified VLPs preparations showed only one band with a size of approximately 7.3 kilobases. The susceptibility to RNase 1 and S1 digestions, resistance to DNase and thermal denaturation behaviour of the viral genome indicated that it is a single-stranded RNA. To our knowledge, isometric single-stranded RNA VLPs isolated from Shiitake mushroom mycelia have not been reported before.

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“…However these viruses were not further characterized. Later on, Ushiyama et al [88] detected three morphologically different polyhedral virus particles with diameters of roughly 25, 30 and 39 nm in both sporophores and mycelia of L. edodes. As being the most prevalent one, the 39 nm virus particles were studied more in detail and revealed to be composed of an outer capsid shell and an inner core containing a dsRNA genome [88,87].…”

Section: Viruses Of Lentinula Edodesmentioning

confidence: 99%

“…Later on, Ushiyama et al [88] detected three morphologically different polyhedral virus particles with diameters of roughly 25, 30 and 39 nm in both sporophores and mycelia of L. edodes. As being the most prevalent one, the 39 nm virus particles were studied more in detail and revealed to be composed of an outer capsid shell and an inner core containing a dsRNA genome [88,87]. Besides, this virus were mostly found to localize in the cytoplasm and in the vacuoles of hyphal cells, either with randomly dispersed or with aggregated forms [86].…”

Section: Viruses Of Lentinula Edodesmentioning

confidence: 99%

Viruses infecting macrofungi

Şahin

Akata

2018

VirusDis.

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Ever since their discovery just about 56 years ago in the cultivated mushroom , many more viruses infecting fungi have been identified in a wide range of fungal taxa. With mostly being asymptomatic, especially the ones that are detrimental to their phytopathogenic hosts are intensively studied due to their considerable importance in developing novel plant protection measures. Contrary to the rapid accumulation of notable data on viruses of plant pathogenic microfungi, much less information have hitherto been obtained in regards to the viruses whose hosts are macrofungi. According to the current literature, only more than 80 distinct viruses bearing either linear dsRNA or linear positive sense ssRNA genome and infecting a total number of 34 macrofungal species represented with four and 30 have been identified so far. Among these 34 macrofungal species, 14 are cultivated edible and wild edible mushroom species. According to the 10th ICTV (International Committee on Taxonomy of Viruses) Report, macrofungal viruses with linear dsRNA genome are classified into five families (, ,, and) and macrofungal viruses with linear positive sense ssRNA genome are classified into seven families (, ,, ,, and). In this review, following a brief overview of some general characteristics of fungal viruses, an up to date knowledge on viruses infecting macrofungal hosts were presented by summarizing the previous, recent and prospective studies of the field.

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Evidence for double-stranded RNA from polyhedral virus-like particles in Lentinus edodes (Berk.) Sing

Cited by 23 publications

References 10 publications

Detection and distribution of six linear mitochondrial plasmids in the shiitake mushroom, Lentinula edodes

Detection and distribution of six linear mitochondrial plasmids in the shiitake mushroom, Lentinula edodes

The detection of single‐stranded RNA in an isometric virus‐like particle from Shiitake mushroom [Lentinus edodes (Berk.) Sing.]

Viruses infecting macrofungi

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