Evidence for genetic relationship between RNA and DNA viruses from the sequence homology of a putative polymerase gene of Bluetongue virus with that of vaccinia virus: conservation of RNA polymerase genes from diverse species

Roy, Polly; Fukusho, Akio; Ritter, Gerd; Lyon, Deborah

doi:10.1093/nar/16.24.11759

Cited by 38 publications

(12 citation statements)

References 20 publications

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“…Sequence (Morozov, 1989); B, cryptic viruses; C, dsRNA viruses. SNBV, Sindbis virus; AIMV, alfalfa mosaic virus; BMV, brome mosaic virus; BMSV, barley stripe mosaic virus; TRV, tobacco rattle virus; TMV, tobacco mosaic virus; BNYVV, beet necrotic yellow vein virus; TYMV, turnip yellow mosaic virus; PVX, potato virus X (Morozov, 1989); ROT, bovine rotavirus (L1) (Cohen et al, 1989); REO reovirus serotype 3 (L1) (Wiener & Joklik, 1989); BTV, bluetongue virus serotype 10 (L1) (Roy et al, 1988); RDV, rice dwarf virus (S1) (Suzuki et al, 1992); ScV, (Diamond et al, 1989); HAV, hypovirulence-associated virus (Koonin et al, 1991 ;Shapira et al, 1991); IBDV, infectious bursal disease virus (Morgan et al, 1988); and qb6, bacteriophage ~6 Bruenn, 1991).…”

Section: Short Communicationmentioning

confidence: 99%

Nucleotide sequence of beet cryptic virus 3 dsRNA2 which encodes a putative RNA-dependent RNA polymerase

Xie

Antoniw

White

1993

Journal of General Virology

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Section: Short Communicationmentioning

confidence: 99%

Nucleotide sequence of beet cryptic virus 3 dsRNA2 which encodes a putative RNA-dependent RNA polymerase

Xie

Antoniw

White

1993

Journal of General Virology

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“…Because this strain is defective in MAKIO, it is unable to maintain L-A dsRNA, even though the normal L-A proteins are supplied from the cDNA vector. M1 is stably maintained, however, and is present in viral particles (21 (4)(5)(6)(7)(8)(9). The region shown here has the most extensive homology among these proteins, but conserved patterns extend well beyond these in both N-terminal and C-terminal directions (1, 3).…”

Section: Methodsmentioning

confidence: 89%

“…1). The same patterns are present in most of the dsRNA viruses whose polymerase segment has been sequenced, including the L-A virus of Saccharomyces cerevisiae (4), reovirus (5), bluetongue virus (6), and rotavirus (7). Two other dsRNA viruses [46 phage (8) and infectious bursal disease virus (9)] show a less clear fit to the pattern.…”

mentioning

confidence: 85%

RNA-dependent RNA polymerase consensus sequence of the L-A double-stranded RNA virus: definition of essential domains.

Ribas

Wickner²

1992

Proc. Natl. Acad. Sci. U.S.A.

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The L-A double-stranded RNA virus of Saccharomyces cerevisiac makes a gag-pol fusion protein by a -1 ribosomal frameshift. The pol amino acid sequence includes consensus patterns typical of the RNA-dependent RNA polymerases (EC 2.7.7.48) of (+) strand and double-stranded RNA viruses of animals and plants. We have carried out "alaninescanning mutagenesis" of the region of L-A including the two most conserved polymerase motifs, SG...T...NT..N (. = any amino acid) and GDD. By constructing and analyzing 46 different mutations in and around the RNA polymerase consensus regions, we have precisely dermed the extent of domains and specific residues essential for viral replication. Assuming that this highly conserved region has a common secondary structure among different viruses, we predict a largely fl-sheet structure.The (+) strand RNA viruses of animals and plants share amino acid sequence patterns in their RNA-dependent RNA polymerase (EC 2.7.7.48) regions (1-3) (Fig. 1). The same patterns are present in most of the dsRNA viruses whose polymerase segment has been sequenced, including the L-A virus of Saccharomyces cerevisiae (4), reovirus (5), bluetongue virus (6), and rotavirus (7). Two other dsRNA viruses [46 phage (8) and infectious bursal disease virus (9)] show a less clear fit to the pattern. The extent of these common sequence patterns suggests that there is a common structure and function among the more than 50 viral enzymes for which sequence data are available and that information obtained about one of them may be applicable to others. While different enzymes must recognize different sites on viral RNA and interact with different viral and host proteins, there are also common functions which they must carry out, including binding of Mg2' and rNTPs, holding onto the template RNA chain, and the chain elongation reaction itself. It is likely that these conserved sequence patterns are part of domains responsible for such common functions. The L-A dsRNA virus of yeast replicates by a conservative mechanism with (+) and (-) strands made inside the viral particle at different points in the replication cycle (reviewed in ref. 10). In vitro systems for replication, transcription, and packaging are available, and the signals for the replication step and for packaging have been defined (11)(12)(13)(14). L-A encodes its 70-kDa major coat protein (called gag) (4, 15) and a 170-kDa gag-pol fusion protein (4, 16) formed by a -1 ribosomal frameshift indistinguishable in mechanism from that used by retroviruses for a similar purpose (17). The pol region of the gag-pol fusion protein (4) has all the characteristic sequence patterns identified by Kamer and Argos (1) as typical of (+) strand RNA viral RNA-dependent RNA poly- We have defined the regions surrounding the two most highly conserved RNA polymerase consensus patterns that are necessary for viral propagation. We show that, although these domains are highly conserved among a broad range of viruses in both primary structure and predicted secondary structure, t...

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“…Towards this end we have sequenced cDNA clones of the genomic RNA species. Eight of the 10 dsRNA segments of BTV-10 have already been cloned and sequenced (Purdy et al, , 1986Ghiasi et al, 1985 ;Lee & Roy, 1986, 1987Yu et al, 1987Yu et al, , 1988Roy et al, 1988). In this paper we complete the sequence analyses by describing the molecular cloning and nucleotide sequence of the remaining two small RNA segments, $8 and $9.…”

Section: Introductionmentioning

confidence: 99%

Completion of the Sequence of Bluetongue Virus Serotype 10 by the Characterization of a Structural Protein, VP6, and a Non-structural Protein, NS2

Fukusho

Yamaguchi

et al. 1989

Journal of General Virology

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Evidence for genetic relationship between RNA and DNA viruses from the sequence homology of a putative polymerase gene of Bluetongue virus with that of vaccinia virus: conservation of RNA polymerase genes from diverse species

Cited by 38 publications

References 20 publications

Nucleotide sequence of beet cryptic virus 3 dsRNA2 which encodes a putative RNA-dependent RNA polymerase

Nucleotide sequence of beet cryptic virus 3 dsRNA2 which encodes a putative RNA-dependent RNA polymerase

RNA-dependent RNA polymerase consensus sequence of the L-A double-stranded RNA virus: definition of essential domains.

Completion of the Sequence of Bluetongue Virus Serotype 10 by the Characterization of a Structural Protein, VP6, and a Non-structural Protein, NS2

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