Evidence for multipotent endodermal stem/progenitor cell populations in human gallbladder

Activation of biliary tree stem cells within peribiliary glands in primary sclerosing cholangitis

“…All counts were performed in at least five different ducts (magnification, x20) for each specimen [7,9,13]. For immunohistochemistry, slides were scanned by a digital scanner (AperioScanscope CS System, Aperio Technologies, Inc, Oxford, UK) and processed by an ImageScope.…”

“…An algorithm was used to quantify the proportion of positive pixel area for a single fluorophore, or the area with co-localisation of two fluorophores [7,9,13].…”

“…3). 13 The Hh pathway is involved in fibrogenesis, epithelial-to-mesenchymal transition (EMT), and carcinogenesis [15]. We evaluated the expression of Hh pathway signalling proteins in PBGs in EHBDs/large IHBDs.…”

Cell sources for regenerative medicine of the liver and endoderm organs: strategies and perspectives

“…Another possible source for stem/progenitor cells for liver regenerative medicine is represented by the extrahepatic biliary tree, including the gallbladder and the hepato-pancreatic ampulla (22,23). A key advantage is their ready availability from adult or fetal donors and from cholecystectomized patients in comparison with the scarcity of marginal livers usable for the isolation of adult HpSCs.…”

Tissue Complex of Adult Pancreatic Duct and Vascular Endothelial Cells Promotes In Vitro Differentiation into Insulin-Producing Cells

“…Stem-like cells isolated from adult pancreatic cells are known to exhibit a certain degree of plasticity that under in vitro conditions they potentially differentiate into pancreatic endocrine cells including β cells [1][2][3]. Despite the therapeutic potential of these tissue-residing stem-like cells, it has not yet been implemented as a proof of concept for transplantation-based cell therapy for diabetes based on two major reasons.…”