Evidence for programmed odontoblast process retraction after dentine exposure in the rat incisor

Mahdee, Anas F; Eastham, Jane; Whitworth, John; Gillespie, James

doi:10.1016/j.archoralbio.2017.10.001

Cited by 8 publications

(8 citation statements)

References 45 publications

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“…As described previously (Sasaki & Garant, 1996;Shahidi et al 2015;Linde & Goldberg, 1993;Li et al, 2018;Maude et al, 2018), in both the molar and incisor, smaller processes branched from the sides of the main process (Figure 2b, arrows). These small processes often approached and appeared to contact similar processes from adjacent main processes.…”

Section: Resultssupporting

confidence: 80%

Odontoblast processes of the mouse incisor are plates oriented in the direction of growth

Shuhaibar

Hand

Terasaki

2020

The Anatomical Record

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Odontoblast processes are thin cytoplasmic projections that extend from the cell body at the periphery of the pulp toward the dentin-enamel junction. The odontoblast processes function in the secretion, assembly and mineralization of dentin during development, participate in mechanosensation, and aid in dentin repair in mature teeth. Because they are small and densely arranged, their three-dimensional organization is not well documented. To gain further insight into how odontoblast processes contribute to odontogenesis, we used serial section electron microscopy and three-dimensional reconstructions to examine these processes in the predentin region of mouse molars and incisors. In molars, the odontoblast processes are tubular with a diameter of 1.8 μm. The odontoblast processes near the incisor tip are similarly shaped, but those midway between the tip and apex are shaped like plates. The plates are radially aligned and longitudinally oriented with respect to the growth axis of the incisor. The thickness of the plates is approximately the same as the diameter of molar odontoblast processes. The plates have an irregular edge; the average ratio of width (midway in the predentin) to thickness is 2.3 on the labial side and 3.6 on the lingual side. The plate geometry seems likely to be related to the continuous growth of the incisor and may provide a clue as to the mechanisms by which the odontoblast processes are involved in tooth development.

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Section: Resultssupporting

confidence: 80%

Odontoblast processes of the mouse incisor are plates oriented in the direction of growth

Shuhaibar

Hand

Terasaki

2020

The Anatomical Record

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“…Therefore, the present study used the term enamel organ for the whole cells including the epithelial and connective tissue surrounding and employed rat mandibular incisor teeth to investigate the structural and functional relationships between these cells during the process of enamel maturation. Since the rat mandibular incisor is a continuous growing tooth, it offers good model in studying the whole sequences of amelogenesis (Warshawsky and Smith 1974) within proper size and easily dissected tooth sample (Mahdee et al 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, rabbit IgG monoclonal (EPR25A) isotype control (1:500 Abcam cat# ab172730, UK) and normal mouse IgG1 (1:500 Santa Cruz Biotechnology cat# sc-3877 UK) were also employed. (ii) by using the secondary uorescent antibodies without the tissue speci c primary markers (Mahdee et al 2018).…”

Section: Methodsmentioning

confidence: 99%

Structural and functional relations between the connective tissue and epithelium of enamel organ and their role during enamel maturation

2021

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The morphological and possible functional interactions between the connective tissue and epithelial elements of enamel organ were examined during the maturation phase, using immunohistochemical techniques. Decalci ed mandibular sections (10µm) including incisors from Wistar rats ages 10-12 weeks were used. Sections were incubated with one or two primary antibodies targeting cell cytoskeleton (vimentin, α-actin, α-tubulin), dendritic marker (OX6), gap junctions (cx-43), enzymes (nitric-oxide synthase (nos1) and cyclooxygenase (cox1)), and ion transporters (Na + /H + exchanger (NHE1) and Na + /Ca 2+ exchanger (NCX)) for 24h, before incubation with the appropriate conjugated uorescent secondary antibodies. Sections were then examined by uorescence microscopy. Haematoxylin-eosin slides were also employed. Cellular heterogeneity and morphological modulations were identi ed within the epithelial and connective tissue elements of the enamel organ suggesting complex cellular interactions and indicating the use of enamel organ term to represent all these regions. Also, some ion transportation activity, and nos1 and cox1 signalling pathways have been identi ed, indicating intercellular communication between these regions. A hypothesis was suggested, to explain the morphological modulation of ameloblasts and papillary cells during enamel maturation aimed to increase the transporting membrane surface area to accomplish faster and bulker ion transportation to achieve controlled pH and to direct Ca 2+ towards enamel. Connective tissue covering epithelial cells of the enamel organ showed morphological and physiological interaction during enamel maturation, suggesting new functional concepts.

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“…The immunohistochemical techniques are well established in our previous work (Mahdee et al . , ), with antibodies thoroughly optimized. However, no quantitative immunohistochemical methods were employed, because of tissue complexity and regional variations within the pulp which impair analysis accuracy.…”

Section: Discussionmentioning

confidence: 99%

“…It has recently been suggested that cytoskeletal structural changes occur during development, maturation, ageing, trauma and repair within rat molar and incisor samples (Mahdee et al . ). However, the cellular signalling mechanisms that control these changes are not well understood.…”

Section: Introductionmentioning

confidence: 97%

Evidence for changing nerve growth factor signalling mechanisms during development, maturation and ageing in the rat molar pulp

et al. 2018

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Evidence for programmed odontoblast process retraction after dentine exposure in the rat incisor

Cited by 8 publications

References 45 publications

Odontoblast processes of the mouse incisor are plates oriented in the direction of growth

Odontoblast processes of the mouse incisor are plates oriented in the direction of growth

Structural and functional relations between the connective tissue and epithelium of enamel organ and their role during enamel maturation

Evidence for changing nerve growth factor signalling mechanisms during development, maturation and ageing in the rat molar pulp

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