Evidence from transcriptome analysis unravelled the roles of eyestalk in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Farhadi, Ardavan; Liu, Yan; Xu, Chang; Han, Tao; Wang, Xiaodan; Li, Erchao

doi:10.1016/j.ygcen.2022.114120

Cited by 18 publications

(7 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the eyestalk, the expression of LV-ACE decreased at 6 h, 12 h, and 72 h of short-term low salinity stress. A similar result was observed in our previous study, mRNA sequencing showed downregulation in the expression level of LV-ACE in the eyestalk of L. vannamei after long-term low salinity stress ( 13 ). The downregulation of LV-ACE in the eyestalk (but not in other organs) under short-term salinity stress could be related to the quick response of the eyestalk as an important regulatory organ for facing stressful situations.…”

Section: Discussionsupporting

confidence: 91%

“…The fragments of LV-ACE , LV-APN , LV-AT 1 R , and LV-RR genes were screened and identified from our transcriptome data (Accession number: SRP048814) by using blast comparison ( 13 ). Total RNA was extracted using TRIzol reagent (Invitrogen, Shanghai, China) following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…In a recent study, the transcriptome analysis revealed that the RAS pathway was activated in Giant Freshwater Prawn ( Macrobrachium rosenbergii ) under hypotonic stress ( 12 ). Our previous study showed that the RAS pathway was suppressed under low salinity in the eyestalk of the Pacific white shrimp Litopenaeus vannamei ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

“…Various adaptive mechanisms must be triggered in L. vannamei to tolerate a wide range of salinity changes. In our previous study, transcriptome analysis revealed that the RAS pathway and its related genes might play important roles in salinity adaptation in L. vannamei , but the detailed mechanisms remain unknown ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Farhadi

Liu

et al. 2022

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

The renin-angiotensin system (RAS) is a hormonal system that plays an important role in the regulation of blood pressure and cardiovascular homeostasis in mammals. In fishes, the RAS pathway participates in osmoregulation and salinity adaptation. However, the role of the RAS pathway in invertebrates, particularly in crustaceans, remains unknown. In this study, four key genes of the RAS pathway (LV-ACE, LV-APN, LV-AT1R, and LV-RR) were cloned, characterized, and their expression levels were detected in the eyestalk, hepatopancreas, and muscle of Litopenaeus vannamei during long-term and short-term low salinity stress. The results showed that LV-ACE, LV-APN, LV-AT1R, and LV-RR encode 666, 936, 175, and 323 amino acids, respectively. Low salinity stress downregulated the expression levels of LV-ACE, LV-APN, LV-AT1R, and LV-RR in L. vannamei, indicating that the RAS pathway was suppressed under low salinity. Moreover, these genes play important roles in the regulation of drinking rate, controlling urine output, blood glucose, and blood pressure, indicating that their downregulation probably affected the homeostasis of shrimps. These findings provide novel insights into the mechanism of salinity adaptation in L. vannamei.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Farhadi

Liu

et al. 2022

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The transcriptome analysis also deepens knowledge of the molecular mechanisms triggered in P. vannamei cultivated under different salinities. Differentially expressed genes related to ion transport and amino metabolism, and energy has been observed when freshwater or low salinity conditions are compared with seawater-cultivated shrimp, suggesting an increased energy demand for osmoregulation and homeostasis (Zhang et al 2016, Farhadi et al 2022.…”

Section: Relationship Between Metabolism and Osmoregulationmentioning

confidence: 99%

Metabolic and osmoionic effects of the recombinant crustacean hyperglycemic hormone (rCHH-A) of the Pacific white shrimp Penaeus vannamei on specimens acutely exposed to extreme salinities

Balderas-González

Ponce-Rivas

Dı́az

et al. 2023

Lat. Am. J. Aquat. Res.

View full text Add to dashboard Cite

The crustacean hyperglycemic hormone (CHH) is a multifunctional neuropeptide that plays a central role in crustacean metabolism and physiology. Experiments were conducted to examine the metabolic and osmoionic capabilities of the white shrimp Penaeus vannamei injected with its recombinant CHH-A (rCHH-A) peptide and acutely transferred from iso- (26 ppt) to hypo- (10 ppt) and hyper-osmotic (40 ppt) conditions. Hemolymph glucose, protein, osmoregulatory capacity (OC), and ionoregulatory capacity (IC) for sodium, chloride, potassium, and calcium were evaluated at four post-injection times (0.5, 1, 2, and 24 h). The rCHH-A peptide had hyperglycemic activity in all salinity conditions, obtaining maximum values at 1 h post-injection. However, in shrimp transferred to hyper-osmotic condition, rCHH-A caused the most significant reductions in OC (2 h), chloride IC (2 h), and total proteins (0.5-2 h) compared to the phosphate-buffered saline (PBS) control. Contrastingly, in shrimp transferred to hypo-osmotic conditions, rCHH-A decreased OC significantly from 2-24 h, strongly decreased chloride and potassium IC at 1 h post-injection, and increased total protein concentration in the hemolymph (1-2 h) when compared to PBS control. Concerning calcium, the rCHH-A injection decreased calcium IC at 10 ppt (1 h) and 26 ppt (2 h), providing insight into a potential role for CHH-A in calcium regulation. The results suggest that glucose and protein mobilization could enhance energy for osmo-ionic regulation under extreme osmotic conditions. This research study contributed to understanding crustacean endocrinology in P. vannamei and related euryhaline crustaceans. Further research should be performed to understand the osmo- and ionoregulatory mechanisms of the different CHH variants in crustaceans exposed to other stress conditions and the relationship with intermediary energy metabolism regulation.

show abstract

Molecular characterization and DNA methylation analysis of carbonic anhydrase (Sp-CA) in the mud crab Scylla paramamosain: Its potential osmoregulation role under carbonate alkalinity stress

Wang

Fang

Liu

et al. 2023

Aquaculture Reports

View full text Add to dashboard Cite

Evidence from transcriptome analysis unravelled the roles of eyestalk in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Cited by 18 publications

References 71 publications

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Metabolic and osmoionic effects of the recombinant crustacean hyperglycemic hormone (rCHH-A) of the Pacific white shrimp Penaeus vannamei on specimens acutely exposed to extreme salinities

Molecular characterization and DNA methylation analysis of carbonic anhydrase (Sp-CA) in the mud crab Scylla paramamosain: Its potential osmoregulation role under carbonate alkalinity stress

Contact Info

Product

Resources

About