Evidence of multiple pathways capable of emitting peroxyoxalate chemiluminescence using a charge coupled devicespectrometer

Park, Paul S.; Rho, Tae-Ho D.; Kim, Young Teck; Ko, Seok Oh; Schlautman, Mark A.; Carraway, Elizabeth R.; Lee, Jihoon

doi:10.1039/c1cc10554j

Cited by 21 publications

(12 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fig. The possible CRET mechanism in guanine chemiluminescence reaction may be similar to chemically initiated electron exchange luminescence (CIEEL) mechanism of peroxyoxalate chemiluminescence (Lee et al, 2002a(Lee et al, , 2002bMaruyama et al, 2013;Park et al, 2011). The ratio determined with 100 s measurement was the smallest.…”

Section: Interaction Between Tba and Go In Guanine Chemiluminescencementioning

confidence: 83%

Rapid and simple G-quadruplex DNA aptasensor with guanine chemiluminescence detection

Cho¹,

Park

Chong³

et al. 2014

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

Cost-effective and sensitive aptasensor with guanine chemiluminescence detection capable of simply quantifying thrombin in human serum was developed using thrombin aptamer (TBA), one of the G-quadruplex DNA aptamers, without expensive nanoparticles and complicated procedures. Guanines of G-quadruplex TBA-conjugated carboxyfluorescein (6-FAM) bound with thrombin do not react with 3,4,5-trimethoxylphenylglyoxal (TMPG) in the presence of tetra-n-propylammonium hydroxide (TPA), whereas guanines of free TBA- and TBA-conjugated 6-FAM immobilized on the surface of graphene oxide rapidly react with TMPG to emit light. Thus, guanine chemiluminescence in 5% human serum with thrombin was lower than that without thrombin when TBA-conjugated 6-FAM was added in two samples and incubated for 20 min. In other words, the brightness of guanine chemiluminescence was quenched due to the formation of G-quadruplex TBA-conjugated 6-FAM bound with thrombin in a sample. High-energy intermediate, capable of emitting dim light by itself, formed from the reaction between guanines of TBA and TMPG in the presence of TPA, transfers energy to 6-FAM to emit bright light based on the principle of chemiluminescence energy transfer (CRET). G-quadruplex TBA aptasensor devised using the rapid interaction between TBA-conjugated 6-FAM and thrombin quantified trace levels of thrombin without complicated procedures. The limit of detection (LOD = background + 3 × standard deviation) of G-quadruplex TBA aptasensor with good linear calibration curve, accuracy, precision, and recovery was as low as 12.3 nM in 5% human serum. Using the technology reported in this research, we expect that various types of G-quadruplex DNA aptasensors capable of specifically sensing a target molecule such as ATP, HIV, ochratoxin, potassium ions, and thrombin can be developed.

show abstract

Section: Interaction Between Tba and Go In Guanine Chemiluminescencementioning

confidence: 83%

Rapid and simple G-quadruplex DNA aptasensor with guanine chemiluminescence detection

Cho¹,

Park

Chong³

et al. 2014

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among the several peroxides proposed so far as the high-energy intermediates (HEIs) capable of generating the excited fluorophores during their decomposition, the four-membered cyclic peroxides, such as 4-aryloxy-4-hydroxy-1,2-dioxetane-3-one (DOT) and 1,2-dioxetne-3,4-dione (DOD), have been the most frequently proposed [3,4,6,7,9,10,14,19,21,22]. However, these intermediates have never been captured nor isolated.…”

Section: Introductionmentioning

confidence: 99%

The Hammett correlation between distyrylbenzene substituents and chemiluminescence efficiency providing various ρ-values for peroxyoxalate chemiluminescence of several oxalates

Maruyama

Narita

Motoyoshiya

2013

Journal of Photochemistry and Photobiology A: Chemistry

View full text Add to dashboard Cite

Peroxyoxalate chemiluminescence (PO-CL) was investigated using eight oxalates with various phenol moieties and the distyrylbenzene (DSB) fluorophores with various substituents. The ρ-values in the Hammett correlation between the substituent constants (σ p + ) of the DSBs and the singlet chemiexcitation yields (Φ S ) for the PO-CL reactions varied from -0.50 to -1.01 depending on the oxalate structure, and the reactive oxalates tended to afford the higher absolute ρ-values but with a few exceptions. Based on the CIEEL mechanism, these experimental observations suggest that the aryloxy groups still remain in the 1,2-dioxetanones (DOTs), which are the postulated high-energy intermediates, and control the electronic properties of DOTs as electron-acceptors. The LUMO energies of the DOTs calculated by the ab initio method with a B3LYP/6-31g(d) basis set reveal that the lower the DOT-LUMO energies, the higher the absolute ρ-values were provided for the corresponding oxalates, as predicted by the frontier molecular orbital (FMO) theory. Thus, the chemical species interacting with the DSBs would be not unitary and will be DOTs.2

show abstract

“…Interaction between fluorescent dye and high-energy intermediate formed from the reaction of guanine and TMPG is similar to that between fluorescent dye and high-energy intermediate from peroxyoxalate chemiluminescence reaction (Augusto et al, 2013;Lee et al, 2003;Maruyama et al, 2013;Park et al, 2011) even though the former is generated by the intra-CRET (Cho et al, 2014), whereas the latter is observed by the inter-CRET.…”

Section: Sequence Effect Of Ssdnas In Guanine Chemiluminescencementioning

confidence: 76%

Rapid aptasensor capable of simply diagnosing prostate cancer

Cha¹,

Cho²,

Kim

et al. 2014

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

Evidence of multiple pathways capable of emitting peroxyoxalate chemiluminescence using a charge coupled devicespectrometer

Abstract: Peroxyoxalate chemiluminescence (PO-CL) spectra obtained simultaneously and continuously using a CCD spectrometer provide evidence of the complexity of PO-CL reactions.

Cited by 21 publications

References 15 publications

Rapid and simple G-quadruplex DNA aptasensor with guanine chemiluminescence detection

Rapid and simple G-quadruplex DNA aptasensor with guanine chemiluminescence detection

The Hammett correlation between distyrylbenzene substituents and chemiluminescence efficiency providing various ρ-values for peroxyoxalate chemiluminescence of several oxalates

Rapid aptasensor capable of simply diagnosing prostate cancer

Contact Info

Product

Resources

About