Evidence of multiple point mutations in Theileria annulata cytochrome b gene incriminated in buparvaquone treatment failure

Chatanga, Elisha; Mosssad, Ehab; Abubaker, Hazem A.; Alnour, Sondos Amin; Katakura, Ken; Nakao, Ryo; Salim, Bashir

doi:10.1016/j.actatropica.2018.12.041

Cited by 39 publications

(50 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On contrary to our findings, a recent study by Chatanga et al (2019) found that only 3 non-synonymous mutations on 50 isolates at codon 146 (50/50), 129 (18/50) and 227 (3/50), while no mutations were present in the Qo2 site in all studied sequences. The possible explanation is that the samples used in its study were collected from animals treated with buparvaquone regardless of whether or not these animals responded to the therapy.…”

Section: Resultscontrasting

confidence: 99%

Mutations in Theileria Annulata Cytochrome B Gene Associated with Buparvaquone Resistance in Cattle, Egypt

Yousef¹

2020

PVJ

View full text Add to dashboard Cite

Buparvaquone is now the principle medicinal drug utilized against tropical theileriosis. The current study was conducted to determine the potential association between the resistance against buparvaquone and the occurrence of mutations in the Cytochrome b (Cyto b) gene of Theileria annulata (T. annulata) in cattle under Egyptian conditions. We sequenced the Cyto b gene of T. annulata of 6 blood samples collected from 1 responding and 5 non-responding cattle to buparvaquone treatment. The analysis revealed that two mutations occurred in codons 253 and 262 within the quinol oxidation (Qo2) drug-binding site of the five resistant isolates. Two mutations in the Qo1 site were found together in the buparvaquone sensitive isolate only. Our findings suggest that the failure of buparvaquone therapy could be attributed to the presence of single or double point mutations at the Qo2 site of T. annulata Cyto b gene.

show abstract

Section: Resultscontrasting

confidence: 99%

Mutations in Theileria Annulata Cytochrome B Gene Associated with Buparvaquone Resistance in Cattle, Egypt

Yousef¹

2020

PVJ

View full text Add to dashboard Cite

show abstract

“…From 1990s, several cases of treatment failure with buparvaquone were reported in Tunisia (M. Darghouth, unpublished data), leading then to suspect the emergence of T. annulata resistance stocks to buparvaquone. This phenomenon was confirmed later in vivo (Mhadhbi et al, 2010), and the resistance mechanism was associated with single point mutations affecting the cytochrome b gene (Sharifiyazdi, Namazi, Oryan, Shahriari, & Razavi, 2012;Mhadhbi, Chaouch, Ajroud, Darghouth, & BenAbderrazak, 2015;Chatanga et al, 2019). However, another study revealed that the mutation of the gene encoding the parasite enzyme PIN1 represents an additional resistance mechanism to buparvaquone, which acts in the absence of the mutation, by inhibiting the proliferation of the T. annulata host cell via blocking the PIN1 enzyme (Marsolier et al, 2015).…”

Section: Towards Improvement Of the Control Of Tropic Al Theilerios Ismentioning

confidence: 86%

Current status of tropical theileriosis in Northern Africa: A review of recent epidemiological investigations and implications for control

Gharbi

Darghouth

Elati

et al. 2020

Transbound Emerg Dis

View full text Add to dashboard Cite

Tropical theileriosis caused by the apicomplexan hemoparasite Theileria annulata is a tick‐borne disease that constraints livestock production in parts of Europe, Asia and Africa. Four Hyalomma tick species transmit T. annulata in at least eight Africa countries (Mauritania, Morocco, Algeria, Tunisia, Egypt, Sudan, South Sudan and Ethiopia). The two dominant T. annulata vector ticks present in Africa, H. scupense and H. anatolicum, underlie two different patterns of transmission, which in turn greatly influence the epidemiology of tropical theileriosis. H. dromedarii and H. lusitanicum are also capable of transmitting T. annulata in North Africa, but their roles are associated with specific production systems and agro‐ecological contexts. The emergence of resistance to the most widely used theilericidal compound, buparvaquone, continues to limit the effectiveness of chemotherapy. In addition, acaricide use is increasingly becoming unsustainable. Deployable T. annulata attenuated live vaccines established from local strains in Tunisia, Sudan and Egypt are available, and recent work has indicated that these vaccines can be protective under conditions of natural transmission. However, vaccination programmes may vary over space and time due to differences in the prevalence of disease amongst cattle populations, as well seasonal variation in vector activity. We review recent descriptive and analytical surveys on the epidemiology of T. annulata infection with reference to (a) demographic aspects such as breeds and ages of cattle herds previously exposed to distinct T. annulata infection pressures and (b) seasonal dynamics of tick activity and disease transmission. We then discuss how the wider endemic patterns that we delineate can underpin the development and execution of future vaccination programmes. We also outline options for integrated control measures targeting tick vectors and husbandry practices.

show abstract

“…Buparvaquone resistance has been reported with increasing frequency and now represents a serious challenge to efficient livestock production (Mhadhbi et al, 2010). Current understanding of the mechanisms and genetic basis of buparvaquone resistance in T. annulata is limited (Chatanga et al, 2019; Mhadhbi et al, 2015; Sharifiyazdi et al, 2012), with no functional link between mutations in the candidate cytochrome b locus and resistance phenotype having been demonstrated. Although previous studies have described multiple genotypes and high levels of genetic diversity (Al-Hamidhi et al, 2015; Weir et al, 2011), there is a lack of information on changes in response to drug selection pressure, fitness costs of mutations, and gene flow.…”

Section: Introductionmentioning

confidence: 99%

“…Buparvaquone resistance has been reported in several countries and now represents a serious challenge to efficient livestock production (Mhadhbi et al, 2010). Although mutations in the candidate cytochrome b locus linked to resistance phenotype have been demonstrated, a causal relationship has not been established (Chatanga et al, 2019; Mhadhbi et al, 2015; Sharifiyazdi et al, 2012). Previous studies have described multiple genotypes and high levels of genetic diversity in T. annulata (Al-Hamidhi et al, 2015; Weir et al, 2011), although there is a lack of information on the genotypic diversity of drug-resistant parasites and whether there are fitness costs associated with mutations of the cytochrome b locus.…”

Section: Introductionmentioning

confidence: 99%

Contrasting population genetics of cattle- and buffalo-derivedTheileria annulatacausing tropical theileriosis

Chaudhry¹,

Ali

Zheng

et al. 2020

Preprint

View full text Add to dashboard Cite

The present study was designed to improve understanding of Theileria annulata in sympatric water buffalo and cattle in the Punjab province of Pakistan. The prevalence of tropical theileriosis is high, buparvaquone resistance is widespread, and vaccine protection is poor in the field. Better understanding is, therefore, needed of the factors that influence the genetics of T. annulata populations both within its hosts and in its overall populations. Here we utilise a panel of six satellites and a mitochondrial cytochrome b marker to explore the multiplicity of T. annulata infection and patterns of emergence and spread of different parasite genotypes. Parasite materials were collected from infected animals in defined regions, where water buffalo and cattle are kept together. Our results show that T. annulata is genetically more diverse in cattle- than in water buffalo-derived populations (the mean numbers of unique satellite alleles were 13.3 and 1.8 and numbers of unique cytochrome b locus alleles were 65 and 27 in cattle- and water buffalo-derived populations, respectively). The data show a high level of genetic diversity among the individual host-derived populations (the overall heterozygosity (He) indices were 0.912 and 0.931 in cattle, and 0.874 and 0.861 in buffalo, based on satellite and cytochrome b loci, respectively). When considered in the context of high parasite transmission rates and frequent animal movements between different regions, the predominance of multiple T. annulata genotypes, with multiple introductions of infection in the hosts from which the parasite populations were derived, may have practical implications for the spread of parasite genetic adaptations; such as those conferring vaccine cross-protection against different strains affecting cattle and buffalo, or resistance to antiprotozoal drugs.

show abstract

Evidence of multiple point mutations in Theileria annulata cytochrome b gene incriminated in buparvaquone treatment failure

Cited by 39 publications

References 29 publications

Mutations in Theileria Annulata Cytochrome B Gene Associated with Buparvaquone Resistance in Cattle, Egypt

Mutations in Theileria Annulata Cytochrome B Gene Associated with Buparvaquone Resistance in Cattle, Egypt

Current status of tropical theileriosis in Northern Africa: A review of recent epidemiological investigations and implications for control

Contrasting population genetics of cattle- and buffalo-derivedTheileria annulatacausing tropical theileriosis

Contact Info

Product

Resources

About