2005
DOI: 10.1101/gad.1335705
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Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A

Abstract: The transcription factor Spo0A is a master regulator for entry into sporulation in the bacterium Bacillus subtilis, but it has been uncertain whether activation of Spo0A is sufficient to trigger development. Spo0A, a member of the response regulator family of gene-control proteins, is activated by phosphorylation via a multicomponent phosphorelay in response to conditions of nutrient limitation. We now report that sporulation can be triggered with high efficiency in cells in the exponential phase of growth in … Show more

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Cited by 269 publications
(344 citation statements)
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“…Strains used and generated in this study are listed in Table S1. Detailed information about the ASI system (Phy-spank-kinA) can be found elsewhere (21,28). CFP, YFP, and mCherry reporter genes were amplified by PCR using the primers listed in Table S2.…”
Section: Experimental Materials and Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Strains used and generated in this study are listed in Table S1. Detailed information about the ASI system (Phy-spank-kinA) can be found elsewhere (21,28). CFP, YFP, and mCherry reporter genes were amplified by PCR using the primers listed in Table S2.…”
Section: Experimental Materials and Methodsmentioning
confidence: 99%
“…Such perturbations can be controllably achieved by using synthetic biology methods to rewire the naturally occurring networks (26). To apply these methods for phosphorelay, we use a synthetic artificial sporulation initiation (ASI) system, which allows for tunable control of Spo0A∼P activity by artificially tuning the KinA level from an isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible promoter (27,28) (Fig. 1B).…”
mentioning
confidence: 99%
“…To examine the importance of the phosphorelay in this process, the constitutively active mutant variant of Spo0A (Sad67) was tested. This mutant protein does not require phosphorylation by the phosphorelay to activate sporulation gene expression (23,24). Thus, a strain harboring the sad67 gene under the control of the spac promoter lacks any putative positive-feedback loop acting on spo0A transcription or Spo0A phosphorylation (Fig.…”
Section: B Subtilis Undergoes Aging But This Process Does Not Affecmentioning
confidence: 99%
“…One possible experiment would be to artificially increase the number of cells which display a specific trait (e.g. [48]). If the reduced phenotypic heterogeneity results in an increased mean fitness as well as a decrease in the variation of fitness, then the original population did not display bet hedging concerning that trait.…”
Section: Excluding Bet Hedgingmentioning
confidence: 99%