Evidence that Heteroencapsidation Between Two Potyviruses Is Involved in Aphid Transmission of a Non-Aphid-Transmissible Isolate from Mixed Infections

Bourdin, Danièle; Lecoq, Hervé

doi:10.1094/phyto-81-1459

Cited by 57 publications

(30 citation statements)

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“…Aphid transmission of ZYMV-NAT, a CP-deficient aphid nontransmissible strain, has also been described in presence of PRSV. In this case, heterologous encapsidation of the ZYMV RNA by PRSV CP was responsible for the aphid transmission of ZYMV (Bourdin & Lecoq, 1991). 'Heteroencapsidation' also occurred when ZYMV-NAT infected transgenic N. benthamiana plants expressing the CP of an aphid transmissible strain of plum pox virus (PPV) .…”

Section: Aphid Transmissionmentioning

confidence: 99%

Zucchini yellow mosaic virus

Desbiez¹,

Lecoq²

1997

Plant Pathology

224

172

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Zucchini yellow mosaic potyvirus (ZYMV), first isolated in Italy in 1973, described in 1981, and then identified in all continents within a decade, is one of the most economically important viruses of cucurbit crops. It is efficiently aphid-transmitted in a nonpersistent manner and it is also seed-borne in zucchini squash, which could have contributed to its rapid spread worldwide. Biological variability has been observed among ZYMV isolates, concerning host range, symptomatology and aphid transmissibility. More recent studies also revealed a serological and molecular variability. The survival of ZYMV in areas where cucurbits are not grown throughout the year remains to be elucidated, because very few natural over-wintering hosts have been identified so far. Partial control of ZYMV can be achieved by limiting transmission of the virus to the crops by aphids, using adapted cultural practices. Cross-protection with a mild strain has been shown to be effective against most ZYMV isolates. Resistance genes found in cucurbit germplasms are currently being introduced into cultivars with good agronomical characteristics. Pathogen-derived resistance strategies using the expression of ZYMV genes in transgenic plants have also been developed and appear promising. Nevertheless, the high biological variability of ZYMV justifies a careful evaluation of the deployment of genetic control strategies in order to increase their durability.

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Section: Aphid Transmissionmentioning

confidence: 99%

Zucchini yellow mosaic virus

Desbiez¹,

Lecoq²

1997

Plant Pathology

224

172

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“…When the helper protein is not deficient, transmission is determined by properties of the capsid protein itself (Hobbs & Me Laughlin 1990). Immunosorbent EM (decoration) suggests that in doubly-infected plants, virions are composed of two capsid proteins (Bourdin & Lecoq 1991). Analogous transmission mechanisms are thought to explain discemination by aphids of viruses that are not transmissible separately but are when present in mixture (Murant et al 1976: Simons 1976.…”

Section: Discussionmentioning

confidence: 99%

“…The luteoviruses and the poty viruses provide examples of both systems, phenotypic mixing does not necessarily result in unstable or biologically impaired virions and transcapsidation has been observed in the field (Creamer & Falk 1992;Farinelli et al 1992;Wen & Lister 1992). Transencapsidation is potentially important because of the role played by capsid proteins in vector attachment at least for some viruses (e.g., potyviruses -Bourdin & Lecoq 1991;Ravelonandro et al 1993) but also because capsid proteins protect ssRNAs and are, in some instances, essential facilitators of systemic plant invasion by virion nucleic acids (e.g., Wellink & van Kammen 1989).…”

Section: Introductionmentioning

confidence: 99%

Transgenic resistance genes from nepoviruses: Efficacy and other properties

Cooper

Edwards

Rosenwasser

et al. 1994

New Zealand Journal of Crop and Horticultural Science

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Evidence of RNA-RNA recombination or transcapsidation was sought in transgenic tobacco (Nicotiana tabacum L. 'Xanthi-nc') containing the 3'-non coding sequence (1.3 kb) from a birch isolate of cherry leaf roll nepovirus (CLRV). Following mechanical inoculation with a rhubarb isolate of the virus, no evidence of recombination was obtained using polymerase chain reactions whether the transgenic gene produced transcripts in the sense or the antisense orientation. Transgenic tobacco containing the CLRV-derived sequence (in either orientation) did not lessen the pathological effects of the rhubarb isolate of the virus althdugh there was amelioration of the disease caused by the birch isolate. When expressed as a transgenic gene in tobacco, the capsid coding sequence of arabis mosaic nepovirus (ArMV) lessened infectibility by nematodes carrying that virus. This construct did not influence the pathogenicity of potato virus Y o (PVY 0 ), tobacco rattle tobravirus (PRN), alfalfa mosaic ilarvirus (A1MV), or CLRV, did not alter the levels of their accumulation and did not render their virions more or less prone to recognition by ArMVspecific antibodies. Uninoculated tobacco expressing the ArMV capsid protein contained ArMV-like particles which co-purified with RNA that hybridised with cDNA to tobacco mRNA and to DNA complementary to the capsid coding sequence.

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“…Two strains of ZYMV differing in HC-Pro were used in the present study. (i) A strain that is non-aphid-transmissible owing to coat protein deficiency, designated ZYMV-NAT, has a biologically active HC-Pro (Antignus et al, 1989;Bourdin & Lecoq, 1991;Lecoq et al, 1991a). ZYMV-NAT has been used to prepare a full-length clone, and infectious in vitro transcripts (Gal-On et al, 1991); it was rendered aphid-transmissible by introducing a mutation in the CP gene and has therefore been named ZYMV-NAT t (Gal-On et al, 1992).…”

Section: Methodsmentioning

confidence: 99%

Mutations in the helper component protease gene of zucchini yellow mosaic virus affect its ability to mediate aphid transmissibility

Huet

Gal‐On

Meir

et al. 1994

Journal of General Virology

120

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The nucleotide sequence of the helper component protease (HC-Pro) genes of three zucchini yellow mosaic virus (ZYMV) strains has been compared with that of a helper-deficient strain of ZYMV-HC. The comparisons revealed three unique deduced amino acid differences. Two of these mutations were located in regions which are conserved in other potyviruses. The role of these mutations in aphid transmissibility was examined by exchanging DNA fragments of part of the deficient HCPro gene with the respective section within the gene of the infectious full-length clone of the aphid-transmissible ZYMV. The first exchange included two of the three mutations, the first coding for a change from Asp to Gly (in a non-conserved region) and the second coding for a change from Arg to Ile [within the Phe-Arg-Asp-Lys (FRNK) conserved box]. This exchange resulted in a reduced transmission (20-6% for the mutated virus compared with 57-4% in the normal ZYMV when acquired from plants and 37.2 % compared with 83"1%, respectively, when acquired from membranes). The second exchange incorporated a single mutation [conferring a change from Thr to Ala within the Pro-Thr-Lys (PTK) conserved box]. This single mutation resulted in almost total loss of HC activity in aphid transmission both from plants and from membranes. The Lys residue in the conserved Lys-Ile-Thr-Cys (KITC) box, which is related to loss of HC activity in potato virus Y, tobacco vein mottling virus and in the Michigan strain of ZYMV, is unchanged in the helper-deficient ZYMV. It is therefore proposed that more than one site in HC-Pro may be functionally related to aphid transmissibility. The possible reasons for the role of these mutations in helper activity in aphid transmission of ZYMV are discussed.

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Evidence that Heteroencapsidation Between Two Potyviruses Is Involved in Aphid Transmission of a Non-Aphid-Transmissible Isolate from Mixed Infections

Cited by 57 publications

References 0 publications

Zucchini yellow mosaic virus

Zucchini yellow mosaic virus

Transgenic resistance genes from nepoviruses: Efficacy and other properties

Mutations in the helper component protease gene of zucchini yellow mosaic virus affect its ability to mediate aphid transmissibility

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