Evolución de técnicas de diagnóstico de virus fitopatógenos

GonzÃ¡lez-Garza, Ramiro

doi:10.18781/r.mex.fit.1706-1

Cited by 5 publications

(4 citation statements)

References 9 publications

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“…Advancement in molecular methods and the widening range of techniques have contributed to the GRP Germplasm Health Unit's advancement of the protocol established for CsCMV diagnosis by qPCR. This allows updating, refining, and using more efficient techniques such as qPCR when detecting any virus of interest [ 46 ]. The safe movement and distribution of germplasm are strictly linked to the requirement of diagnostic tests to determine the germplasm's phytosanitary status, because one of the risks involved in the transfer of plant material is the possible movement of pests and diseases to unreported sites [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Quantitative detection of cassava common mosaic virus for health certification of cassava (Manihot esculenta Crantz) germplasm using qPCR analysis

Niño-Jimenez,

López-López,

Cuervo-Ibáñez

2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Quantitative detection of cassava common mosaic virus for health certification of cassava (Manihot esculenta Crantz) germplasm using qPCR analysis

Niño-Jimenez,

López-López,

Cuervo-Ibáñez

2024

Heliyon

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“…Once collected, the nematodes were euthanized in a water bath (55 • C for 4 min) (Solidsteel, Araras, Brazil) and preserved in a fixed solution of triethanolamine and formalin. Subsequently, they were identified through examination of their perineal configuration, as outlined in reference [21], and their identity was confirmed using isoenzyme electrophoresis (esterase), following the procedure detailed in reference [22].…”

Section: Collection and Preparation Of Nematode Inocula And Acquiring...mentioning

confidence: 99%

Efficiency of Vinasse Application on Root-Knot Nematodes in Soybean

Leite,

Almeida,

Fonseca

et al. 2023

Agronomy

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Vinasse is not only effectively used in pest control but also creates a conducive environment for the growth of antagonistic microorganisms. Thus, this study aimed to evaluate the potential of vinasse applied via soil for the management of root-knot nematodes in soybean culture. The experimental design was entirely random, in a factorial scheme (2 × 6), consisting of two species of nematodes, Meloidogyne incognita and M. javanica, under vinasse application at five concentrations (20, 40, 60, 80, and 100%) and one control (water), with five repetitions. Soybean plants Intacta cv. M-Soy 8644 IPRO were inoculated with 4000 eggs/juveniles of each species separately. At 60 days after the first application of vinasse, evaluations of parasitism and agronomic characteristics in soybean were performed. Stillage resulted in the highest average values for root volume and root fresh mass in plants inoculated with M. incognita, showing respective increases of 24.33% and 14.92% compared to plants inoculated with M. javanica. However, concentrations exceeding 60% had a detrimental effect on all agronomic variables of soybean. For parasitism, an interaction among the factors was observed, with a significant effect (p < 0.01) for most of the evaluated variables, except for the number of eggs in the soil. The concentration equivalent to 60% vinasse promoted a sharp reduction in parasitism for the two nematode species, making reproduction on plant roots unfeasible.

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“…The cucumber mosaic virus (CMV) has only been detected using serological techniques like ELISA. However, these techniques have been displaced by molecular strategies that are more sensitive (González-Garza, 2017).…”

Section: Introductionmentioning

confidence: 99%

Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops

2020

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The chili pepper (Capsicum spp. L.) is a vegetable of economic importance that has been affected worldwide by the cucumber mosaic virus (CMV), a pathogen that causes a devastating disease in this crop. The aim of this research was the detection and characterization of CMV in chili pepper crops in Valle del Cauca, Colombia. Leaves of three chili pepper varieties (tabasco, cayenne and habanero) with viral symptoms were collected in four municipalities of Valle del Cauca. Total RNA was purified and a fragment of capsid protein (CP) from CMV was amplified by RT‑PCR. Then, it was sequenced and bioinformatically analyzed, and from these sequences, specific primers were designed. From 71 chili pepper samples collected in Palmira, Yumbo, Vijes and Yotoco, 37 were positive for CMV (52.1%). The CMV chili pepper sequence analysis showed that they had their highest identity (98.5%) with a CMV isolated from bananas in Ecuador. Specific primers designed for CMV chili pepper showed greater sensitivity for detecting this virus (64.7% vs. 52.1%). The CMV chili pepper CP analysis indicated that it could be transmitted by the species Aphis gossypii. This r the first time, the molecular characterization of CMV in three chili pepper varieties.

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Evolución de técnicas de diagnóstico de virus fitopatógenos

Cited by 5 publications

References 9 publications

Quantitative detection of cassava common mosaic virus for health certification of cassava (Manihot esculenta Crantz) germplasm using qPCR analysis

Quantitative detection of cassava common mosaic virus for health certification of cassava (Manihot esculenta Crantz) germplasm using qPCR analysis

Efficiency of Vinasse Application on Root-Knot Nematodes in Soybean

Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops

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