Evolution and Biological Evaluation of Matrinic Derivatives with Amantadine Fragments As New Anti-Influenza Virus Agents

Niu, Tianyu; Zhao, Xiao-Qiang; Jiang, Jing; Yan, Haiyan; Li, Yinghong; Tang, Sheng; Li, Yuhuan; Song, Dan–Qing

doi:10.3390/molecules24050921

Cited by 11 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The score ranged from zero, signifying no CPE, to four representing the most severe CPE for the inactivated virus’s CPE [ 18 ]. The most substantial alteration in CPE scoring is demonstrated in Figure-2 , primarily occurring on the 1 st day of the second passage.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Inactivation of an Indonesian isolate of foot-and-mouth disease virus using formaldehyde

Tobing,

Rantam,

Widiyatno

et al. 2024

Vet World

View full text Add to dashboard Cite

Background and Aim: Foot-and-mouth disease (FMD) is a highly contagious viral disease that endangers livestock and the environment with significant economic consequences. This study aimed to validate the inactivation of the Indonesian isolate of foot-and-mouth disease virus (FMDV) with various formaldehyde concentration. Materials and Methods: The experiment started with FMDV being adapted on BHK-21 cells until cytopathic effects (CPE) appeared. The biological titer of the virus was determined using the 50% tissue culture infectious dose (TCID50) assay. The virus was inactivated by exposing the isolate to different formaldehyde (FA) concentrations (0.025%, 0.05%, 0.1%, and 0.2%) at 37°C for 24 h, and residual infectivity was assessed using CPE scoring of reinoculated BHK-21 cells. Results: 72 h post-inoculation, the virulence of the FMDV isolate was indicated by complete CPE on BHK-21 monolayer cells, with a TCID50 value of 109/mL; CPE scoring did not signify significant differences (p < 0.05) among 0.025%, 0.05%, 0.1%, 0.2% FA, and the negative control. All treatment groups showed significant differences (p < 0.05) from the positive control (C+). FA concentrations inactivated the FMDV isolate under the given conditions. 0.025% and 0.05% FA continued to display CPE through the third passage, while 0.2% FA did not significantly differ from 0.1% FA (p > 0.05). 0.1% FA is the optimal concentration for safely and effectively inactivating FMDV. Conclusion: All of the formaldehyde concentrations can completely inactivate the FMDV isolate, with the most optimal and safe concentration being 0.1%. Keywords: foot-and-mouth disease virus, formaldehyde inactivation, vaccine development.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Every day, the cells were checked for cytopathic and other effects. CPE severity levels were assessed on a scale from zero to four, where zero represented no observable CPE and four denoted 75%–100% observable CPE [ 18 ]. The third passage effectively inactivated the virus, as no CPE was detected at its end [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

Inactivation of an Indonesian isolate of foot-and-mouth disease virus using formaldehyde

Tobing,

Rantam,

Widiyatno

et al. 2024

Vet World

View full text Add to dashboard Cite

show abstract

“…Second to the large superfamily of G-protein coupled receptors (GPCRs), ion channels are among the most soughtafter membrane-protein targets by the United States Federal Drug Administration. Ion-channel modulators in particular have shown therapeutic potential and successes, for example as blockers of influenza M2 channels (Moorthy et al, 2014;Sakai et al, 2018;Niu et al, 2019). It is expected that the current pandemic will prompt further research into this important area, with obvious therapeutic opportunities.…”

Section: Therapeutic Potential Of Channel Blockers Acting On Cov Ion mentioning

confidence: 99%

Structural biology of coronavirus ion channels

Barrantes

2021

Acta Crystallogr D Struct Biol

View full text Add to dashboard Cite

Viral infection compromises specific organelles of the cell and readdresses its functional resources to satisfy the needs of the invading body. Around 70% of the coronavirus positive-sense single-stranded RNA encodes proteins involved in replication, and these viruses essentially take over the biosynthetic and transport mechanisms to ensure the efficient replication of their genome and trafficking of their virions. Some coronaviruses encode genes for ion-channel proteins – the envelope protein E (orf4a), orf3a and orf8 – which they successfully employ to take control of the endoplasmic reticulum–Golgi complex intermediate compartment or ERGIC. The E protein, which is one of the four structural proteins of SARS-CoV-2 and other coronaviruses, assembles its transmembrane protomers into homopentameric channels with mild cationic selectivity. Orf3a forms homodimers and homotetramers. Both carry a PDZ-binding domain, lending them the versatility to interact with more than 400 target proteins in infected host cells. Orf8 is a very short 29-amino-acid single-passage transmembrane peptide that forms cation-selective channels when assembled in lipid bilayers. This review addresses the contribution of biophysical and structural biology approaches that unravel different facets of coronavirus ion channels, their effects on the cellular machinery of infected cells and some structure–functional correlations with ion channels of higher organisms.

show abstract

“…The insertion of norbornene and its derivatives into the organic molecules is creating a great impact in the field of medicinal and pharmaceutical chemistry . These types of insertion reactions are established with particular functional groups in the literature .…”

mentioning

confidence: 99%

Domino Palladium-Catalyzed Double Norbornene Insertion/Annulation Reaction: Expeditious Synthesis of Overcrowded Tetrasubstituted Olefins

2019

View full text Add to dashboard Cite

A Pd-catalyzed domino process involving a double norbornene insertion/annulation reaction was developed for the expeditious synthesis of overcrowded olefins. In this one-pot reaction, four new C–C bond formations were achieved by three consecutive Heck carbopalladations and C–H activation across the CC triple bond of 2-alkynyl bromobenzenes with two norbornene rings via a reactive vinylic-Pd(II) species. This protocol provides a step-economical synthetic system to access the structurally identical molecular machine motifs of overcrowded olefins with high yields.

show abstract

Evolution and Biological Evaluation of Matrinic Derivatives with Amantadine Fragments As New Anti-Influenza Virus Agents

Cited by 11 publications

References 23 publications

Inactivation of an Indonesian isolate of foot-and-mouth disease virus using formaldehyde

Inactivation of an Indonesian isolate of foot-and-mouth disease virus using formaldehyde

Structural biology of coronavirus ion channels

Domino Palladium-Catalyzed Double Norbornene Insertion/Annulation Reaction: Expeditious Synthesis of Overcrowded Tetrasubstituted Olefins

Contact Info

Product

Resources

About