Evolution and Classification of Oncogenic Human Papillomavirus Types and Variants Associated with Cervical Cancer

Chen, Zigui; Freitas, Luciana Bueno de; Burk, Robert D.

doi:10.1007/978-1-4939-2013-6_1

Cited by 27 publications

(22 citation statements)

References 12 publications

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“…Novel HPV types are given a unique number only after the whole genome has been cloned and deposited with the International HPV Reference Center [1,4,6]. The Papillomavirus nomenclature at the species level and above is determined by the PV study group of the International Committee on Taxonomy of Viruses [1,4,70].…”

Section: Update On the International Hpv Reference Centermentioning

confidence: 99%

Molecular methods for identification and characterization of novel papillomaviruses

Kocjan

Bzhalava

Forslund

et al. 2015

Clinical Microbiology and Infection

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Papillomaviruses (PV) are a remarkably heterogeneous family of small DNA viruses that infect a wide variety of vertebrate species and are aetiologically linked with the development of various neoplastic changes of the skin and mucosal epithelia. Based on nucleotide similarity, PVs are hierarchically classified into genera, species and types. Novel human PV (HPV) types are given a unique number only after the whole genome has been cloned and deposited with the International HPV Reference Center. As of 9 March 2015, 200 different HPV types, belonging to 49 species, had been recognized by the International HPV Reference Center. In addition, 131 animal PV types identified from 66 different animal species exist. Recent advances in molecular techniques have resulted in an explosive increase in the identification of novel HPV types and novel subgenomic HPV sequences in the last few years. Among PV genera, the γ-PV genus has been growing most rapidly in recent years with 80 completely sequenced HPV types, followed by α-PV and β-PV genera that have 65 and 51 recognized HPV types, respectively. We reviewed in detail the contemporary molecular methods most often used for identification and characterization of novel PV types, including PCR, rolling circle amplification and next-generation sequencing. Furthermore, we present a short overview of 12 and 10 novel HPV types recently identified in Sweden and Slovenia, respectively. Finally, an update on the International Human Papillomavirus Reference Center is provided.

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Section: Update On the International Hpv Reference Centermentioning

confidence: 99%

Molecular methods for identification and characterization of novel papillomaviruses

Kocjan

Bzhalava

Forslund

et al. 2015

Clinical Microbiology and Infection

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“…One sample was negative for HPV-16 but positive for HPV-35. HPV type 35 is closely related phylogenetically to HPV-16 29 . Tumors testing positive for low-risk (non-oncogenic) types were considered HPV−.…”

Section: Methodsmentioning

confidence: 99%

Cancer as the Disintegration of Robustness: Population-Level Variance in Gene Expression Identifies Key Differences Between Tobacco- and HPV-Associated Oropharyngeal Carcinogenesis

Ben-Dayan¹,

MacCarthy²,

Belbin³

et al. 2015

Archives of Pathology &Amp; Laboratory Medicine

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Context Oropharyngeal squamous cell carcinoma (OPSCC) is associated both with tobacco use and human papillomavirus (HPV) infection. It is argued that carcinogen-driven tumorigenesis is a distinct disease from its virally-driven counterpart. We hypothesized that tumorigenesis is the result of a loss of genotypic robustness resulting in an increase in phenotypic variation in tumors compared to adjacent histologically normal tissues, and that carcinogen-driven tumorigenesis results in greater variation than their virally-driven counterparts. Objective To examine the loss of robustness in carcinogen- and virally-driven OPSCC samples, and to identify potential pathways involved. Design We used coefficients of variation (CVs) for mRNA and microRNA expression to measure the loss of robustness in OPSCC samples. Tumors were compared to matched normal tissues, and were further categorized by HPV and patient smoking status. Weighted gene co-expression networks were constructed for genes with highly variable expression among the HPV- tumors from smokers. Results We observed more genes with variable mRNA expression in tumors compared to normal tissues, regardless of HPV and smoking status, and more microRNAs with variable expression in HPV− and HPV+ tumors from smoking patients than from non-smokers. For both the mRNA and microRNA data, we observed more variance among HPV− tumors from smokers compared to HPV+ tumors from non-smokers. The gene co-expression network construction highlighted pathways that have lost robustness in carcinogen-induced tumors but appear stable in virally-induced tumors. Conclusions Using CVs and co-expression networks, we identified multiple altered pathways that may play a role in carcinogen-driven tumorigenesis.

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“…Human papillomaviruses (HPVs) consist of a heterogeneous group of non‐enveloped, capsid‐enclosed dsDNA viruses, 55 nm in diameter that exhibit a different tropism for mucosal or cutaneous epithelia . Today more than 170 HPV genotypes have been completely characterized with each one of them displaying different life cycle features and carcinogenic properties . A phylogenetic tree based on the L1 gene clustered the different HPV genotypes into five genera—alpha, beta, gamma, mu, and nu .…”

Section: Introductionmentioning

confidence: 99%

Association of p16 (CDKN2A) polymorphisms with the development of HPV16‐related precancerous lesions and cervical cancer in the Greek population

Tsakogiannis

Moschonas

Bella

et al. 2017

Journal of Medical Virology

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The tumor suppressor protein p16 plays a fundamental role in cell cycle regulation and exerts a protective effect against tumor growth. Two different polymorphisms at positions 540 and 580 at the 3'UTR of exon 3 of p16 gene are implicated in several types of cancer, while their role in cervical cancer development remains rather vague. In the present study, we investigated for the impact of p16 genotypes/haplotypes on patients' vulnerability to cervical disease and examined whether these factors can be used as progression markers in the Greek population. A total of 96 HPV16 positive samples and histologically confirmed as LSIL (42 samples), HSIL (44 samples), and cervical cancer cases (10 samples) along with 50 control cases were tested. The identification of p16 polymorphisms was performed by PCR-RFLP methodology. The present analysis revealed that women with p16 540 CG/GG genotype are at a 2.7-fold higher risk of developing HPV16-associated HSIL (OR = 2.7, 95%CI: 1.01-6.6, P = 0.028). The G allele can be regarded as a risk factor of developing HSIL in the Greek population (OR = 2.7, 95%CI: 1.2-5.9, P = 0.012). Moreover, p16 polymorphism C580T is not associated with the growth of cervical lesion in Greek patients, while 540G/580C haplotype can be regarded as a risk haplotype of developing HSIL (OR = 3.67, 95%CI: 1.56-8.6, P = 0.0019). Our results demonstrated that p16 C540G polymorphism influence patients' susceptibility to more severe dysplasia and consequently this polymorphism could potentially emerge as a valuable biomarker for HSIL development in the Greek population.

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Evolution and Classification of Oncogenic Human Papillomavirus Types and Variants Associated with Cervical Cancer

Abstract: The nomenclature of human papillomavirus (HPV) is established by the International Committee on Taxonomy of Virus (ICTV). However, the ICTV does not set standards for HPV below species levels. This chapter describes detailed genotyping methods for determining and classifying HPV variants.

Cited by 27 publications

References 12 publications

Molecular methods for identification and characterization of novel papillomaviruses

Molecular methods for identification and characterization of novel papillomaviruses

Cancer as the Disintegration of Robustness: Population-Level Variance in Gene Expression Identifies Key Differences Between Tobacco- and HPV-Associated Oropharyngeal Carcinogenesis

Association of p16 (CDKN2A) polymorphisms with the development of HPV16‐related precancerous lesions and cervical cancer in the Greek population

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