Evolution and expression analysis of the grape (Vitis vinifera L.) WRKY gene family

Guo, Chunfang; Guo, Rongrong; Xu, Xiaozhao; Gao, Min; Li, Xiaoqin; Song, Junyang; Zheng, Yi; Wang, Xiping

doi:10.1093/jxb/eru007

Cited by 259 publications

(271 citation statements)

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“…The WRKY family genes confer multiple functions in plant developmental and physiological processes (Eulgem et al 2000;Miao and Zentgraf 2007;Zhou et al 2011;Guo et al 2014) and biotic and abiotic stress responses (Rushton et al 1996;Eulgem and Somssich 2007;Chen et al 2012). In cucumber, previous reports indicated that under normal growth conditions, there were 48 WRKY genes displaying differential expression in their transcript levels or in their expression patterns, and 23 WRKY genes exhibited expressional difference in response to at least one of three abiotic stresses including cold, drought and salinity) (Ling et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Since the first WRKY gene, SPF1, was cloned from sweet potato (Ishiguro and Nakamura 1994), a large number of WRKY genes were identified and characterized from different plant species, such as tobacco (Yang et al 1999;Chen and Chen 2000), Arabidopsis (Eulgem et al 2000;Dong et al 2003), parsley (Cormack et al 2002), barley (Sun et al 2003), rice (Xie et al 2005;Ross et al 2007), coffee (Ramiro et al 2010), cucumber (Ling et al 2011), poplar (He et al 2012), soybean (Yin et al 2013), physic nut (Xiong et al 2013), brachypodium distachyon (Wen et al 2014), and grape (Guo et al 2014).…”

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The expression patterns of Cucumis sativus WRKY (CsWRKY) family under the condition of inoculation with Phytophthora melonis in disease resistant and susceptible cucumber cultivars

et al. 2015

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, T. 2015. The expression patterns of Cucumis sativus WRKY (CsWRKY) family under the condition of inoculation with Phytophthora melonis in disease resistant and susceptible cucumber cultivars. Can. J. Plant Sci. 95: 1121Á1131. Based on RNA-seq, we analyzed expression patterns of 55 CsWRKYs in the disease-resistant cultivar (JSH) and the disease-susceptible cucumber cultivar (B80) at 0 and 40 h post-inoculation with Phytophthora melonis. After inoculation with P. melonis, in JSH and B80, the number of up-regulated CsWRKY genes was both 25 with 22 commonly up-regulated genes, and there were 22 CsWRKY genes down-regulated in JSH and 23 in B80 with 18 commonly down-regulated genes, and there were eight CsWRKY genes with no or little change in transcript expression in JSH and seven in B80 with four common genes. Among the 55 CsWRKY genes, 17 genes showed significant differences (differences twofold) in the degree of change of gene transcription level between JSH and B80 from RNA-seq data analysis, and the results validated using real-time PCR showed that there were only six genes (CsWRKY2, 20, 26, 35, 44, 52) out of the 17 that exhibited significant differences (differences twofold) with four genes (CsWRKY2, 20, 26, 52) consistent with the data from RNA-seq and two genes (CsWRKY 35, 44) inconsistent with the data from RNA-seq. In addition, the six genes were strongly up-regulated after salicylic acid (SA) treatment with five genes (CsWRKY2, 20, 26, 35, 44) up to peak at 12 h and one gene (CsWRKY52) up to peak at 24 h in expression and that four CsWRKY genes (CsWRKY2, 20, 44, 52) out of six were strongly up-regulated with three genes (CsWRKY2, 20, 44) up to the highest point at 24 h and one gene (CsWRKY52) up after methyl jasmonate (MeJA) treatment. Based on the results above, we predicted CsWRKY2, 20, 26, 35, 44 and 52 may be involved in disease resistance of JSH against Phytophthora melonis by SA and (or) JA signaling pathway(s).Key words: Cucumis sativus, CsWRKY, expression patterns, Phytophthora melonis Xu, X., Wang, R., Chao, J., Lin, Y., Jin, Q., He, X., Luo, S. et Wu, T. 2015. Modes d'expression de la famille de ge`nes WRKY (CsWRKY) de Cucumis sativus apre`s inoculation de Phytophthora melonis chez les cultivars de concombre re´sistant et sensible a`la maladie. Can. J. Plant Sci. 95: 1121Á1131. Se fiant sur la se´quence de l'ARN, les auteurs ont analyse´le mode d'expression de 55 ge`nes CsWRKY chez les cultivars de concombre re´sistant (JSH) et sensible (B80) a`Phytophthora melonis (P. melonis), 0 h et 40 h apre`s inoculation du pathoge`ne. Apre`s inoculation, ils ont de´nombre´25 ge`nes CsWRKY re´gule´s en amont chez JSH et B80, dont 22 qui l'e´taient normalement, ainsi que 23 ge`nes CsWRKY re´gule´s en aval chez JSH et 23 chez B80, dont 18 l'e´tant normalement. S'y ajoutaient 8 ge`nes CsWRKY dont l'expression n'avait pas change´ou tre`s peu apre`s la transcription chez JSH, contre 7 chez B80, y compris quatre ge`nes courants. Sur les 55 ge`nes CsWRKY, 17 avaient subi un changement significatif (plus du doub...

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confidence: 99%

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The expression patterns of Cucumis sativus WRKY (CsWRKY) family under the condition of inoculation with Phytophthora melonis in disease resistant and susceptible cucumber cultivars

et al. 2015

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“…The iPBS marker method has several advantages compared with other retrotransposon markers: iPBSs can discriminate among genotypes without prior sequence knowledge and are highly reproducible due to their primer length and the high stringency achieved by the annealing temperature (Guo et al, 2014b). Indeed, this marker system has been used successfully for several genetic diversity studies in plants, such as apricot (Baranek et al, 2012), Cicer species (Andeden et al, 2013), and Vitis vinifera (Guo et al, 2014a(Guo et al, , 2014b. Chen and Liu (2014) also reported that iPBS and start codon-targeted polymorphism markers are effective marker systems for discriminating genotypes in Myrica rubra.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) accessions through retrotransposon-based interprimer binding sites (iPBSs) markers

Nemli¹,

Kianoosh²,

Tanyolaç³

2015

Turk J Agric For

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IntroductionThe common bean (Phaseolus vulgaris L.) is one of the most ancient legumes and one of the five cultivated species of the genus Phaseolus (Broughton et al., 2003). The common bean originated in Latin America and has become a major food for human consumption (Broughton et al., 2003), providing vital nutrients such as proteins, vitamins, and minerals in diets in many developing countries, especially in Africa (Broughton et al., 2003). Globally, the annual production of green and dry beans is 17 million tons (FAO, 2010) and is almost twice that of the second most important legume, chickpea (Cicer arietinum L.) (Gepts et al., 2008). The common bean, a self-pollinated crop, is a true diploid (n = 11) with a small genome of 588 megabase pairs (Arumuganthan and Earle, 1991).Transposable elements (TEs) are discrete regions of DNA that can move within genomes (Baranek et al., 2012). TEs are important for phylogenetic analysis because they can change their genomic location, creating genomic diversity (Baranek et al., 2012). Recent studies have shown that, depending on its function, a TE in a gene may be conserved in different plant species, subspecies, and cultivars (Xu and Ramakrishna, 2008). TEs can be classified into two major groups according to their mode of transposition: DNA transposons (group I), which replicate directly via a DNA intermediate, and retrotransposons (group II), which replicate through an RNA intermediary (Casacuberta and Santiago, 2003). Retrotransposons play important roles in plant genomes according to genome size (increasing genome size), structure, evolution, variable copy number, and random distribution (Kumar and Bennetzen, 1999). Furthermore, retrotransposons are the most abundant and widely distributed mobile genetic element in eukaryotic genomes and show polymorphism within and between species (Kumar and Bennetzen, 1999). Retrotransposons comprise 35% of the common bean genome (Schmutz et al., 2014). Retrotransposons can be divided into two primary groups according to the presence or absence of a long terminal repeat (LTR): LTR and non-LTR retrotransposons. LTR retrotransposons predominate in plant genomes and can be used as molecular markers due to their ubiquitous distribution, abundant copy number, high heterogeneity, and random nature of insertional polymorphisms resulting from different retrotransposon

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“…Though WRKYGQK is highly conserved in most WRKY members, variant proteins such as WRKYGKK, WRKYGEK, WRKYEDK, WKKYGQK, CRKYGQK, WHQYGLK, WRKYGMK, WSKYGQK, WQKYGQK as well as WIKYGEN were identified in Solanum lycopersicum, Glycine max and Vitis vinifera (Huang et al 2012;BenckeMalato et al 2014;Guo et al 2014). WRKY motif sequence is followed by a C2H2-or C2HC-type zinc finger motif, with sequences of C-X 4-5 -C-X 22-23 -H-X-H and C-X 5-8 -C-X 25-28 -H-X 1-2 -C, however, there are some exceptions, e.g.…”

Section: Discussionmentioning

confidence: 99%

Over-expression of a WRKY transcription factor gene BoWRKY6 enhances resistance to downy mildew in transgenic broccoli plants

Jiang

Qing-e

Liu

et al. 2016

Australasian Plant Pathol.

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WRKY transcription factors play an important role in plant growth, development and immunity. In our study, a WRKY family member gene designated BoWRKY6 was isolated from broccoli (Brassica oleracea var. italica), and its expression was induced by downy mildew (Hyaloperonospora parasitica). Five transgenic broccoli lines over-expressing BoWRKY6 driven by the CaMV 35S promotor were obtained by Agrobacterium tumefaciens mediated transformation, and they demonstrated significant increased resistance to downy mildew, with resistant levels from low to very high. Real timequalitative PCR analysis indicated that expressions of both BoWRKY6 and the pathogenesis-related gene 1 (BoPR1) in transgenic plants were obviously higher than those in WT plants after H. parasitica treatment. Lines of BWK14 and BWK31 exhibited very high resistance to downy mildew, and may serve as promising candidate materials for broccoli molecular breeding in the near future.

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Evolution and expression analysis of the grape (Vitis vinifera L.) WRKY gene family

Abstract: SummaryFifty-nine VvWRKY genes were identified. Phylogenetic tree and synteny analysis revealed the specific evolutionary relationship of these genes. Meanwhile, differential expression patterns indicated their possible roles in specific tissues and under different stresses.

Cited by 259 publications

References 65 publications

The expression patterns of Cucumis sativus WRKY (CsWRKY) family under the condition of inoculation with Phytophthora melonis in disease resistant and susceptible cucumber cultivars

The expression patterns of Cucumis sativus WRKY (CsWRKY) family under the condition of inoculation with Phytophthora melonis in disease resistant and susceptible cucumber cultivars

Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) accessions through retrotransposon-based interprimer binding sites (iPBSs) markers

Over-expression of a WRKY transcription factor gene BoWRKY6 enhances resistance to downy mildew in transgenic broccoli plants

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