Evolution and expression of chimeric POTE–actin genes in the human genome

Lee, Yoomi; Ise, Tomoko; Ha, D. Bui Dang; Fleur, Ashley Saint; Hahn, Yoonsoo; Liu, Xiufen; Nagata, Satoshi; Lee, Byung Kook; Bera, Tapan K.; Pastan, Ira

doi:10.1073/pnas.0608344103

Cited by 43 publications

(49 citation statements)

References 21 publications

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“…The frozen tissue was ground in a mortar set and lysed with RIPA buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 1% Nonidet P-40, 1% deoxycholate, 0.1% SDS, 1 mM PMSF, 1 mg/ml leupeptin, 1 mg/ml aprotinin). After sonication and centrifugation, 4 mg protein was immunoprecipitated by HP8 antibody and detected in Western blotting using PG5 MAb as previously described [4].…”

Section: Immunoprecipitation Western Blottingmentioning

confidence: 99%

“…The length of α helical region varies among paralogs and some paralogs do not contain this region. We have recently reported that several members of the POTE gene family contain an actin retroposon inserted at the carboxyl terminus of an ancestral POTE paralog in the process of gene evolution [4]. The POTE-2α and POTE-2γ actin fusion genes are expressed in embryonic stem cells and breast cancer cell lines [4,5].…”

Section: Introductionmentioning

confidence: 99%

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Expression of POTE protein in human testis detected by novel monoclonal antibodies

Ise

Das

Nagata

et al. 2008

Biochemical and Biophysical Research Communications

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The POTE gene family is composed of 13 highly homologous paralogs preferentially expressed in prostate, ovary, testis and placenta. We produced 10 monoclonal antibodies (MAbs) against 3 representative POTE paralogs: POTE-21, POTE-2γC and POTE-22. One reacted with all 3 paralogs, 6 MAbs reacted with POTE-2γC and POTE-22, and 3 MAbs were specific to POTE-21. Epitopes of all 10 MAbs were located in the cysteine-rich repeats (CRRs) motifs located at the N-terminus of each POTE paralog. Testing the reactivity of each MAb with 12 different CRRs revealed slight differences among the antigenic determinants, which accounts for differences in cross-reactivity. Using MAbs HP8 and PG5 we were able to detect a POTE-actin fusion protein in human testis by immunoprecipitation followed by Western blotting. By immunohistochemistry we demonstrated that the POTE protein is expressed in primary spermatocytes, implying a role in spermatogenesis.

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Section: Immunoprecipitation Western Blottingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of POTE protein in human testis detected by novel monoclonal antibodies

Ise

Das

Nagata

et al. 2008

Biochemical and Biophysical Research Communications

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“…There are no obvious orthologs of POTE gene in other species; however, a distant ancestral gene has been identified in rodents [5]. Recently, we reported that there is an actin retroposon insertion at the carboxyl terminus of one of the ancestral POTE paralogs that occurred before the divergence of Old World monkeys and apes [6]. The resulting gene has duplicated and diverged into several POTE paralogs.…”

Section: Introductionmentioning

confidence: 99%

“…To determine the size of the POTE transcript expressed in hES cells, total RNA from the WA01 cell line was analyzed by northern blot hybridization using a probe 3Ј end of POTE transcript [6]. A specific band of about 7.5 kb in size was detected in WA01 lane, suggesting that the POTE transcript in this cell line is 7.5 kb in size (Fig.…”

Section: Expression Of Pote In Hes Cellsmentioning

confidence: 99%

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Selective POTE Paralogs on Chromosome 2 are Expressed in Human Embryonic Stem Cells

Bera

Fleur

et al. 2008

Stem Cells and Development

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POTE is a primate-specific gene family that encodes cancer testis antigens that contain three domains, although the proteins vary greatly in size. The amino-terminal domain is novel and has three cysteine-rich domains of 37 amino acids. The second and third domains are rich in ankyrin repeats and spectrin-like helices respectively. In humans, 13 highly homologous paralogs are dispersed among eight chromosomes. Some members of the POTE gene family have an actin insertion at the carboxyl end of the protein. The expression of the POTE gene in normal adult tissues is restricted, but several POTE paralogs are frequently expressed in many cancers including breast, prostate, and lung cancers. We show here that POTE is expressed in several human embryonic stem (ES) cell lines. We found that UC06, WA01 and ES03 cell lines express mainly a POTE-2␥ transcript but ES02 and ES04 cell lines predominantly express POTE-2␣. The WA09 cell line expressed both POTE-2␥ and POTE-2␣. There is no detectable POTE gene expression in fetal tissues (ages 16-36 weeks). The POTE paralogs that are expressed in ES cells may have a specific function during lineage-specific differentiation of ES cells. 325

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Proteomic differences in amyloid plaques in rapidly progressive and sporadic Alzheimer’s disease

Nayak²,

et al. 2017

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Rapidly progressive Alzheimer’s disease (rpAD) is a particularly aggressive form of Alzheimer’s disease, with a median survival time of 7–10 months after diagnosis. Why these patients have such a rapid progression of Alzheimer’s disease is currently unknown. To further understand pathological differences between rpAD and typical sporadic Alzheimer’s disease (sAD) we used localized proteomics to analyze the protein differences in amyloid plaques in rpAD and sAD. Label-free quantitative LC-MS/MS was performed on amyloid plaques microdissected from rpAD and sAD patients (n=22 for each patient group) and protein expression differences were quantified. On average, 913±30 (mean±SEM) proteins were quantified in plaques from each patient and 279 of these proteins were consistently found in plaques from every patient. We found significant differences in protein composition between rpAD and sAD plaques. We found that rpAD plaques contained significantly higher levels of neuronal proteins (p=0.0017) and significantly lower levels of astrocyte proteins (p=1.08x10−6). Unexpectedly, cumulative protein differences in rpAD plaques did not suggest accelerated typical sAD. Plaques from patients with rpAD were particularly abundant in synaptic proteins, especially those involved in synaptic vesicle release, highlighting the potential importance of synaptic dysfunction in the accelerated development of plaque pathology in rpAD. Combined, our data provides new direct evidence that amyloid plaques do not all have the same protein composition and that the proteomic differences in plaques could provide important insight into the factors that contribute to plaque development. The cumulative protein differences in rpAD plaques suggest rpAD may be a novel subtype of Alzheimer’s disease.

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Evolution and expression of chimeric POTE–actin genes in the human genome

Cited by 43 publications

References 21 publications

Expression of POTE protein in human testis detected by novel monoclonal antibodies

Expression of POTE protein in human testis detected by novel monoclonal antibodies

Selective POTE Paralogs on Chromosome 2 are Expressed in Human Embryonic Stem Cells

Proteomic differences in amyloid plaques in rapidly progressive and sporadic Alzheimer’s disease

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