Evolution and phyletic distribution of two-component signal transduction systems

Wuichet, Kristin; Cantwell, Brian J.; Zhulin, Igor B.

doi:10.1016/j.mib.2009.12.011

Cited by 234 publications

(208 citation statements)

References 56 publications

Supporting

Mentioning

196

Contrasting

Unclassified

Order By: Relevance

“…Even though its precise function remains unknown, it is plausible to invoke a role as a global regulator, controlling an essential aspect of cell metabolism. In this regard, it is germane to mention that although many two-component systems are dispensable (60,62), at least the WalK/ WalR two-component system in low-GϩC Gram-positive bacteria (16) and the two-component sensor histidine kinase/ response regulator hybrid protein CckA in Caulobacter crescentus (28) are essential proteins. Although the exact roles of RHE_PE00001 and RHE_PE00024 will be explored in future studies, this work reveals the complexities inherent to infer gene essentiality based solely on sequence data.…”

Section: Discussionmentioning

confidence: 99%

Plasmids with a Chromosome-Like Role in Rhizobia

et al. 2011

View full text Add to dashboard Cite

Replicon architecture in bacteria is commonly comprised of one indispensable chromosome and several dispensable plasmids. This view has been enriched by the discovery of additional chromosomes, identified mainly by localization of rRNA and/or tRNA genes, and also by experimental demonstration of their requirement for cell growth. The genome of Rhizobium etli CFN42 is constituted by one chromosome and six large plasmids, ranging in size from 184 to 642 kb. Five of the six plasmids are dispensable for cell viability, but plasmid p42e is unusually stable. One possibility to explain this stability would be that genes on p42e carry out essential functions, thus making it a candidate for a secondary chromosome. To ascertain this, we made an in-depth functional analysis of p42e, employing bioinformatic tools, insertional mutagenesis, and programmed deletions. Nearly 11% of the genes in p42e participate in primary metabolism, involving biosynthetic functions (cobalamin, cardiolipin, cytochrome o, NAD, and thiamine), degradation (asparagine and melibiose), and septum formation (minCDE). Synteny analysis and incompatibility studies revealed highly stable replicons equivalent to p42e in content and gene order in other Rhizobium species. A systematic deletion analysis of p42e allowed the identification of two genes (RHE_PE00001 and RHE_PE00024), encoding, respectively, a hypothetical protein with a probable winged helix-turn-helix motif and a probable two-component sensor histidine kinase/response regulator hybrid protein, which are essential for growth in rich medium. These data support the proposal that p42e and its homologous replicons (pA, pRL11, pRLG202, and pR132502) merit the status of secondary chromosomes.

show abstract

Section: Discussionmentioning

confidence: 99%

Plasmids with a Chromosome-Like Role in Rhizobia

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The P2CS database classified HKs into three major families, classical HKs, HHKs, and unorthodox HKs, as well as the CheA-like HKs [22,26]. There are also hybrid RRs (HRRs) containing HK domains [27,28]. There are 35 different families of RRs based on different types of output domains [22,26,29].…”

Section: How Tcss Workmentioning

confidence: 99%

Two-component signal transduction systems in oral bacteria

Mattos-Graner

Duncan²

2017

Journal of Oral Microbiology

View full text Add to dashboard Cite

We present an overview of how members of the oral microbiota respond to their environment by regulating gene expression through two-component signal transduction systems (TCSs) to support conditions compatible with homeostasis in oral biofilms or drive the equilibrium toward dysbiosis in response to environmental changes. Using studies on the sub-gingival Gram-negative anaerobe Porphyromonas gingivalis and Gram-positive streptococci as examples, we focus on the molecular mechanisms involved in activation of TCS and species specificities of TCS regulons.

show abstract

“…TCSs are present in more than 95% of sequenced bacterial genomes (2,3), and one species can contain tens to more than a hundred TCSs. TCSs regulate a wide range of processes from cell development to virulence.…”

mentioning

confidence: 99%

Experimental Analysis of Functional Variation within Protein Families: Receiver Domain Autodephosphorylation Kinetics

Page

Immormino

et al. 2016

J Bacteriol

View full text Add to dashboard Cite

Plants and microorganisms use two-component signal transduction systems (TCSs) to mediate responses to environmental stimuli. TCSs mediate responses through phosphotransfer from a conserved histidine on a sensor kinase to a conserved aspartate on the receiver domain of a response regulator. Typically, signal termination occurs through dephosphorylation of the receiver domain, which can catalyze its own dephosphorylation. Despite strong structural conservation between receiver domains, reported autodephosphorylation rate constants (k dephos ) span a millionfold range. Variable receiver domain active-site residues D ؉ 2 and T ؉ 2 (two amino acids C terminal to conserved phosphorylation site and Thr/Ser, respectively) influence k dephos values, but the extent and mechanism of influence are unclear. We used sequence analysis of a large database of naturally occurring receiver domains to design mutant receiver domains for experimental analysis of autodephosphorylation kinetics. When combined with previous analyses, k dephos values were obtained for CheY variants that contained D ؉ 2/T ؉ 2 pairs found in 54% of receiver domain sequences. Tested pairs of amino acids at D ؉ 2/T ؉ 2 generally had similar effects on k dephos in CheY, PhoB N , or Spo0F. Acid or amide residues at D ؉ 2/T ؉ 2 enhanced k dephos . CheY variants altered at D ؉ 2/T ؉ 2 exhibited rate constants for autophosphorylation with phosphoramidates and autodephosphorylation that were inversely correlated, suggesting that D ؉ 2/T ؉ 2 residues interact with aspects of the ground or transition states that differ between the two reactions. k dephos of CheY variants altered at D ؉ 2/T ؉ 2 correlated significantly with k dephos of wild-type receiver domains containing the same D ؉ 2/T ؉ 2 pair. Additionally, particular D ؉ 2/T ؉ 2 pairs were enriched in different response regulator subfamilies, suggesting functional significance. T wo-component systems (TCSs) are a prevalent means of signal transduction used by plants and microorganisms to mediate responses to stimuli (1). TCSs are present in more than 95% of sequenced bacterial genomes (2, 3), and one species can contain tens to more than a hundred TCSs. TCSs regulate a wide range of processes from cell development to virulence. Signal transduction by TCSs occurs through the transfer of phosphoryl groups between histidyl and aspartyl residues of different protein components (4). Canonically, the sensory component (the sensor kinase) is a phosphodonor to the response regulator (the response-mediating component) (4). The conserved domain in the response regulator, the receiver domain, functions as a molecular switch. The phosphorylation status of a conserved Asp on the receiver domain corresponds to turning the output response on and off. Typically, receiver domain phosphorylation initiates the output response, and dephosphorylation terminates the response. Dephosphorylation can occur with the assistance of another protein, such as a phosphatase, or by self-catalysis by the receiver domain, which is term...

show abstract

Evolution and phyletic distribution of two-component signal transduction systems

Cited by 234 publications

References 56 publications

Plasmids with a Chromosome-Like Role in Rhizobia

Plasmids with a Chromosome-Like Role in Rhizobia

Two-component signal transduction systems in oral bacteria

Experimental Analysis of Functional Variation within Protein Families: Receiver Domain Autodephosphorylation Kinetics

Contact Info

Product

Resources

About