Evolution and Spatiotemporal Dynamics of Enterovirus A71 Subgenogroups in Vietnam

Thảo, Nguyễn Thị; Donato, Celeste; Trang, Vu Thi Huyen; Kiên, Nguyễn Trung; Trang, Phạm Mai Thuy; Khanh, Tran Quoc; Nguyet, Dang Thi; Sessions, October M.; Cuong, Hoang Quoc; Lan, Phan Trong; Huong, Vû Thi Quê; Doorn, H. Rogier van; Vijaykrishna, Dhanasekaran

doi:10.1093/infdis/jix500

Cited by 19 publications

(33 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The large outbreaks of HFMD caused by the subgenotype C4 were reported in Asia, including China in 2012, southern Vietnam in 2011 and Thailand in 2008‐2009 . In southern Vietnam, HFMD outbreaks were reported with cocirculation of subgenotype C4 and C5 in 2011 . Our study also demonstrated that the subgenotype B5 virus was closely related to variants that caused HFMD outbreaks in southern Vietnam and in Thailand .…”

Section: Discussionsupporting

confidence: 59%

“…The previous studies demonstrated a significant association between the genotypes/subgenotypes and pathogenic proper- 25 In southern Vietnam, HFMD outbreaks were reported with cocirculation of subgenotype C4 and C5 in 2011. 10,11 Our study also demonstrated that the subgenotype B5 virus was closely related to variants that caused HFMD outbreaks in southern Vietnam and in Thailand. 9 EV71 strains 8 18 22 30 31 43 58 77 87 98 121 134 145 167 172…”

Section: Discussionsupporting

confidence: 57%

“…Our study also demonstrated that the subgenotype B5 virus was closely related to variants that caused HFMD outbreaks in southern Vietnam and in Thailand . The subgenotype B5 virus was first reported in southern Vietnam in 2011, then gradually replaced C4 to become the dominant subgenotype causing HFMD epidemics in 2012‐2013 . In this study, 12 B5 strains were identified from Dak Lak of the Central Highlands region.…”

Section: Discussionsupporting

confidence: 57%

“…The phylogenetic analysis indicated that B5 strains in Dak Lak were clustered with strains from southern and northern Vietnam in 2012‐2013 and with strains circulating in Thailand in 2012. B5 strains were supposed to immigrate in Vietnam from Malaysia and Taiwan through Thailand and then they rapidly spread and cocirculated extensively along with other subgenotypes around Vietnam . This also suggested that EV71 strains circulating in Dak Lak were possibly introduced from neighboring provinces in Vietnam.…”

Section: Discussionmentioning

confidence: 99%

“…in 2012-2013. 9,11 In this study, 12 B5 strains were identified from Dak Lak of the Central Highlands region. The phylogenetic analysis indicated that B5 strains in Dak Lak were clustered with strains from southern and northern Vietnam in 2012-2013 and with strains circulating in Thailand in 2012.…”

mentioning

confidence: 96%

See 4 more Smart Citations

Molecular epidemiology analysis of enterovirus 71 strains isolated in Dak Lak, Vietnam, 2011‐2016

Nguyen

et al. 2018

Journal of Medical Virology

View full text Add to dashboard Cite

Human enterovirus 71 (EV71) is the major etiologic agent of hand, foot, and mouth disease (HFMD). EV71 outbreaks have been reported in Dak Lak in recent years, however, the genotypes/subgenotypes information and phylogeny of circulating EV71 strains are limited. The objectives of this study were to determine the genotypes/subgenotypes and investigate the phylogeny of EV71 isolates in Dak Lak over a 6-year period. Viruses were isolated from clinical samples from patients with HFMD. In total, 43 EV71 isolates circulated in Dak Lak during 2011-2016 were used for the phylogenetic analysis using complete VP1 gene. The phylogenetic analysis of the VP1 gene revealed that two major genotypes, B and C, were found. Among the 43 EV71 strains, 29 belonged to subgenotype C4, 2 belonged to subgenotype C5, and 12 belonged to subgenotype B5. Of these, the subgenotype C4 was predominant in 2011-2013 and this was later replaced by the subgenotype B5 in 2014. The subgenotype B5 was dominant between 2014 and 2015, and then C4 recirculated in 2016. Our study also indicated that the subgenotypes C4 and B5 emerged into Dak Lak were closely related to variants causing epidemics of HFMD in the southern and central region of Vietnam and Thailand. Sequence analysis showed that nine amino acid mutations were detected in the VP1 region. Our results identified two significant amino acid substitutions (D31N and E145G/Q) associated with enhancing EV71 virulence.

show abstract

Section: Discussionsupporting

confidence: 59%

Section: Discussionsupporting

confidence: 57%

Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 96%

See 3 more Smart Citations

Molecular epidemiology analysis of enterovirus 71 strains isolated in Dak Lak, Vietnam, 2011‐2016

Nguyen

et al. 2018

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

A 5‐year molecular epidemiology survey of human enterovirus 71 before vaccine application in Yunnan Province, China

Liu

Zhang

et al. 2020

Journal of Medical Virology

View full text Add to dashboard Cite

Enterovirus A71 (EV‐A71) infection is known to cause hand, foot, and mouth disease (HFMD). Last year, an inactivated EV‐A71 whole virus vaccine was used to prevent this disease in Yunnan, China. To obtain a viral genetic background for evaluating vaccine protection and monitor the adaptive evolution of the virus after the vaccination, a 5‐year molecular epidemiology survey was performed before the vaccination. Twenty‐six EV‐A71 strains were separated from 561 stool specimens of patients with serious HFMD. The whole‐genomic sequences of these strains were sequenced. Phylogenetic trees were constructed, and the mutation spectra were analyzed based on these viral sequences. There was no obvious mutation for the circular EV‐A71 strains of the same year. Pathogenic EV‐A71 strains may arise from a “subgroup” randomly each year. Whole‐genomic analyses showed that a hotspot nonsynonymous substitution potentially affecting the immunogenicity of vaccines was found in the 2A gene, but not in genes of the viral capsid proteins, and the genetic diversity of whole viral genomes associated with the incidence of HFMD. Therefore, it will be valuable to monitor the genome‐wide changes of EV‐A71 to detect the adaptive mutations affecting immunogenicity or perform investigations using genetic diversity as a parameter.

show abstract

Genetic characterization of VP1 of coxsackieviruses A2, A4, and A10 associated with hand, foot, and mouth disease in Vietnam in 2012–2017: endemic circulation and emergence of new HFMD-causing lineages

et al. 2020

View full text Add to dashboard Cite

Evolution and Spatiotemporal Dynamics of Enterovirus A71 Subgenogroups in Vietnam

Abstract: This study highlights a persistent transmission network of EV-A71, with specific Asian countries seeding other countries in the region and beyond, emphasizing the need for improved EV-A71 surveillance and detailed genetic and antigenic characterization.

Cited by 19 publications

References 42 publications

Molecular epidemiology analysis of enterovirus 71 strains isolated in Dak Lak, Vietnam, 2011‐2016

Molecular epidemiology analysis of enterovirus 71 strains isolated in Dak Lak, Vietnam, 2011‐2016

A 5‐year molecular epidemiology survey of human enterovirus 71 before vaccine application in Yunnan Province, China

Genetic characterization of VP1 of coxsackieviruses A2, A4, and A10 associated with hand, foot, and mouth disease in Vietnam in 2012–2017: endemic circulation and emergence of new HFMD-causing lineages

Contact Info

Product

Resources

About