Evolution of a centromeric satellite DNA and phylogeny of lacertid lizards

Capriglione, Teresa; Cardone, A; Odierna, Gaëtano; Olmo, Ettore

doi:10.1016/0305-0491(91)90233-4

Cited by 22 publications

(37 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interpopulation variations in C-positive heterochromatin regions have been described in some species of salamanders (genus Hynobius Tschudi, 1838; Ikebe et al, 1987), lizards (Capriglione et al, 1991(Capriglione et al, , 1998YonenagaYassuda et al, 1996), snakes (genus Vipera Linnaeus, 1758; Aprea, et al, 2006) and rodents of the genera Microtus Schrank, 1798 (Kovalskaya et al, 1991) and Peromyscus Gloger, 1841 (Kaidanov, 1996). It is known that the intraspecifi c differences in the amount and distribution of heterochromatin found in taxa of the genus Vipera were taxonomically irrelevant (Aprea et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Features of the karyotypes of Pelophylax ridibundus Pallas, 1771 and Rana macrocnemis Boulenger, 1885 (Amphibia: Ranidae) from Armenia

Martirosyan¹,

Stepanyan²

2009

CCG

View full text Add to dashboard Cite

Abstract. Сhromosomal complements of Pelophylax ridibundus Pallas, 1771 from 9 localities (Northern, Central and South Armenia) and Rana macrocnemis Boulenger, 1885 from one locality (North-West Armenia) have been analyzed. The chromosome sets of P. ridibundus collected from 8 localities showed 2n=26, (10m+12sm+4st; NF=52). A secondary constriction has been observed in all studied individuals on the 10-th chromosome pair showing NOR-positive reaction. C-positive heterochromatin blocks have been observed on long arms of the 2-nd and 10-th pairs of chromosomes (7 localities). In addition, C-heterochromatin blocks have been found on interstitial regions of short arms of the 12-th pairs, as well as in telomeric regions of long arms of the 9-th pairs and on short arms of the 5-th pair in the frogs from 2 localities. The karyotype of P. ridibundus from populations near Ejmiatsin differs from other populations (2n=26, 12m+10sm+4st). Diploid number of chromosomes of R. macrocnemis was also 26 (8m+12sm+6st, NF=52). Blocks of C-positive heterochromatin have been revealed in telomeric parts of the 1-st, 2-nd (p), 3-rd (q), 4-th (q), 6-th, 9-th (p), 10-th (p,q) and 13-th (q) pairs, as well as in interstitial regions of the 1-st and 2-nd pairs of chromosomes. Intrapopulation and interpopulation geographic variations of karyotypes and C-heterochromatin banding patterns of P. ridibundus have been revealed. Karyotypically, morphotypes "macrocnemis" and "camerani" are closely related.

show abstract

Section: Discussionmentioning

confidence: 99%

Features of the karyotypes of Pelophylax ridibundus Pallas, 1771 and Rana macrocnemis Boulenger, 1885 (Amphibia: Ranidae) from Armenia

Martirosyan¹,

Stepanyan²

2009

CCG

View full text Add to dashboard Cite

show abstract

“…The centromeric heterochromatin highlighted by C-banding is likely to represent satellite DNA [e.g., Plohl et al, 2008]. These repetitive elements are usually AT-rich [e.g., Plohl et al, 2008], as also found in some lacertids [e.g., Capriglione et al, 1991;Giovannotti et al, 2014], even though GC-rich satellite DNAs have been described in various animals [e.g., Meneveri et al, 1995;Petrović et al, 2009], including reptiles . The fact that the centromeric heterochromatin of A .…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic Characterization of a Population of Acanthodactylus lineomaculatus Duméril and Bibron, 1839 (Reptilia, Lacertidae), from Southwestern Morocco and Insights into Sex Chromosome Evolution

Giovannotti

Cerioni²,

Slimani³

et al. 2017

Cytogenet Genome Res

Self Cite

View full text Add to dashboard Cite

Acanthodactylus lineomaculatus is now regarded as an ecotype of A. erythrurus with which it has been recently synonymized. Despite the wide range of A. erythrurus, karyological data for this species are scarce and limited to classical cytogenetic studies carried out in individuals from only 2 locations (central Spain and Spanish enclave of Melilla on the northwestern Mediterranean Moroccan coast). Here, for the first time, we cytogenetically characterized individuals of A. lineomaculatus from the southwestern Moroccan Atlantic coast with the aim to increase the karyological knowledge of this wide-ranging species and to assess if any chromosomal changes can be found in this ecotype in comparison to other populations of this species. The diploid number of the individuals investigated is 2n = 38 which is typical of most lacertids. Active NORs were located telomerically in a medium-small pair of chromosomes, and no inactive NORs were detected. C-banding revealed an intensely heterochromatic W chromosome composed of AT-rich (centromere and long arm telomeric region) and GC-rich (most of the long arm) regions, with extended interstitial telomeric sequences. These telomere-like repeats occupy the GC-rich heterochromatin of the W. The DNA composition of the W represents a trait distinguishing A. lineomaculatus (southwestern Morocco) from A. erythrurus from Spain that possess a DAPI-positive (AT-rich) W chromosome. In conclusion, these results add further evidence to the remarkable karyotype conservation in lacertid lizards, although differences in NOR location and in W chromosome structure among populations could suggest an incipient speciation mediated by chromosome changes in this wide-ranging lizard species.

show abstract

“…This result is quite different from what is known for reptilian and non-reptilian satellite DNAs, which show a tendency to AT enrichment (50-65%) [e.g. Capriglione et al, 1991;King and Cummings, 1997;Ciobanu et al, 2001Ciobanu et al, , 2004. The GC-richness of Ava II is also confirmed by CMA 3 staining which produced very bright signals that overlapped the chromosomal distribution of Ava II sat-DNA.…”

Section: Discussionmentioning

confidence: 42%

“…The sequence analysis of Ava II sat-DNA highlighted the presence of short runs of 3 to 5 residues of G and C, whereas homopolymeric A 3-4 and T 3-4 stretches are a common feature in reptilian satDNAs [e.g. Capriglione et al, 1991;Ciobanu et al, 2001Ciobanu et al, , 2004. However, GC-rich satellites have been described for some animal and plant species [Meneveri et al, 1995;Scherthan, 1995;Malykh et al, 2001;Barragán et al, 2002;Cafasso et al, 2003;Petrović et al, 2009] although not for reptiles, while the presence of short G runs has been reported for molluscs [Biscotti et al, 2007] and mammals [Scherthan, 1995].…”

Section: Discussionmentioning

confidence: 99%

Characterisation of a GC-Rich Telomeric Satellite DNA in Eumeces schneideri Daudin (Reptilia, Scincidae)

Giovannotti

Cerioni

Caputo

et al. 2009

Cytogenet Genome Res

View full text Add to dashboard Cite

A hitherto undescribed satellite DNA family (AvaII satDNA) has been isolated and characterised in Eumeces schneideri, a squamate reptile belonging to the family Scincidae. AvaII satDNA is characterised by a monomer length of 208 bp, a GC content of 59% and exhibits a certain degree of CpG methylation. FISH experiments with AvaII satDNA probe produced bright signals (i) at the end of the short arms of all subtelocentric chromosomes except for pair 14, in which the signal was at the end of the long arms, (ii) at the ends of both arms of the small metacentric chromosomes 12, and (iii) in a terminal position on the acrocentric chromosomes 11 and 13. AvaII satDNA repeats were not found in the metacentric pair 3, whereas only a weak interstitial signal occurred in the metacentric pairs 1 and 2. C-banding showed that this satellite represents most of the constitutive heterochromatin in the genome of this skink, and chromomycin A₃ staining produced a clear signal overlapping with the satellite, except for NOR-associated heterochromatin. In addition, quantitative dot blot analysis showed that these repetitive sequences constitute about 3% of the genomic DNA of this lizard. AvaII satDNA sequence analysis revealed the occurrence of short guanine residue stretches for which a function in structural stability of these sequences and a role in recombination with telomeric sequences can be hypothesised. Fibre FISH experiments showed that on some chromatin fibres telomeric sequences and AvaII satellite DNA repeats are intermingled or overlapping.

show abstract

Evolution of a centromeric satellite DNA and phylogeny of lacertid lizards

Cited by 22 publications

References 12 publications

Features of the karyotypes of Pelophylax ridibundus Pallas, 1771 and Rana macrocnemis Boulenger, 1885 (Amphibia: Ranidae) from Armenia

Features of the karyotypes of Pelophylax ridibundus Pallas, 1771 and Rana macrocnemis Boulenger, 1885 (Amphibia: Ranidae) from Armenia

Cytogenetic Characterization of a Population of <b><i>Acanthodactylus lineomaculatus</i></b> Duméril and Bibron, 1839 (Reptilia, Lacertidae), from Southwestern Morocco and Insights into Sex Chromosome Evolution

Characterisation of a GC-Rich Telomeric Satellite DNA in <i>Eumeces schneideri</i> Daudin (Reptilia, Scincidae)

Contact Info

Product

Resources

About