The problems regarding hunter-gatherer/early farmer interactions are quite an important topic in southeast European archaeology. According to the available data, the two economic subsistence systems have coexisted for some 2000 years during the 6th–4th millennia cal BC (Telegin 1985; Lillie et al., 2001). In some areas, hunter-gatherer and early farmer sites are located just a few kilometers apart. The Southern Buh River valley has yielded evidence of Linear Pottery culture, early Trypillia and Trypillia B1 Neolithic settlements as well as hunter-gatherer sites with pottery attributable to the so-called sub-Neolithic or para-Neolithic (Haskevych et al., 2019; Kiosak et al., 2021). Trial-trenches have been opened within some of these sites, which have been radiocarbon-dated from Bern University laboratory (LARA). Soil samples for micromorphological analysis have been collected from these sites to interpret their paleogenetic formation. The soil development is attested since, at least, the beginning of the 5th mill BC, followed by the developed of chernozem soils, which was interrupted by an erosional episode in the end of 5th millennium BC. The available data show that the soils of early farmers arable as are the present day ones. The early farmers were able to exploit relatively heavy soils to cultivate wheat and barley as early as 5250–5050 cal BC. In contrast, the sites of ceramic hunter-gatherers were often located on the soils which formed under wet conditions along seasonally flooded riverbanks, which were almost unsuitable for agricultural practices.