Evolution of Flow Cytometry Technology

Silveira, Guilherme Ferreira

doi:10.4172/1948-5948.1000208

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…In 1953, Walter Coulter developed the first successful Coulter counter, which can detect and count blood cells in a fluid medium. [156] The system utilized the difference in conductivity between cell membrane and saline to detect the electrical signal arising from the cell passing through a pair of electrodes. This system is regarded as the origin of the flow cytometry technique.…”

Section: Flow Cytometry-based Biosensorsmentioning

confidence: 99%

Extracellular Vesicles for the Diagnosis of Cancers

Xue

Fan

et al. 2021

Small Structures

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Extracellular vesicles (EVs) are ultrasmall structures secreted by various types of cells and carry biomarkers such as DNA, RNA, and the protein of the cells that secreted them. Cancer cell-derived EVs can be detected at the very early stage of cancers, and hence the use of EVs for cancer diagnosis has aroused significant interest. This review summarizes the representative strategies of using EVs as biomarkers to detect different types of cancers, evaluates their advantages and challenges, and discusses their development prospects for cancer early diagnosis.

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Section: Flow Cytometry-based Biosensorsmentioning

confidence: 99%

Extracellular Vesicles for the Diagnosis of Cancers

Xue

Fan

et al. 2021

Small Structures

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“…The largest application field was probably that of hematopoietic subsets. For cell suspensions, microscopic examination soon gave way to flow cytometry, a concept that had appeared at the end of the 1960s and has constantly improved since 1 . Of note, this early development benefited from the fluidic technology previously devised for blood cell count instruments, based on the Coulter principle 2 and other parameters such as light scatter.…”

Section: Introductionmentioning

confidence: 99%

Unsupervised flow cytometry analysis in hematological malignancies: A new paradigm

Béné

Lacombe

Porwit

2021

Int J Lab Hematology

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Ever since hematopoietic cells became “events” enumerated and characterized in suspension by cell counters or flow cytometers, researchers and engineers have strived to refine the acquisition and display of the electronic signals generated. A large array of solutions was then developed to identify at best the numerous cell subsets that can be delineated, notably among hematopoietic cells. As instruments became more and more stable and robust, the focus moved to analytic software. Almost concomitantly, the capacity increased to use large panels (both with mass and classical cytometry) and to apply artificial intelligence/machine learning for their analysis. The combination of these concepts raised new analytical possibilities, opening an unprecedented field of subtle exploration for many conditions, including hematopoiesis and hematological disorders. In this review, the general concepts and progress achieved in the development of new analytical approaches for exploring high‐dimensional data sets at the single‐cell level will be described as they appeared over the past few years. A larger and more practical part will detail the various steps that need to be mastered, both in data acquisition and in the preanalytical check of data files. Finally, a step‐by‐step explanation of the solution in development to combine the Bioconductor clustering algorithm FlowSOM and the popular and widely used software Kaluza® (Beckman Coulter) will be presented. The aim of this review was to point out that the day when these progresses will reach routine hematology laboratories does not seem so far away.

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“…Recent decades have witnessed considerable progress in analytical techniques, and flow cytometry emerged as a reliable, repeatable and highly sensitive diagnostic tool that is widely used in both human and veterinary medicine. Traditional spectrophotometric and microscopic methods are being gradually replaced by flow cytometry (Silveira 2015).…”

Section: Introductionmentioning

confidence: 99%

Polish Journal of Veterinary Sciences

Wójcik

Małaczewska

Kaczorek-Łukowska

2019

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The objective of this study was to determine the applicability of the Migratest® kit for evaluating the chemotactic activity of peripheral blood neutrophils in goats. The experiment was performed on 14 goat kids aged 30±2 days, divided into two groups of 7 animals each: C -control group, and E -experimental group, supplemented with β-hydroxy-β-methylbutyrate (HMB), a typical immunostimulant which influences the phagocytic activity of peripheral neutrophils. The feed administered to experimental goat kids was supplemented with HMB at 40 mg/kg BW, whereas control goat kids were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the chemotactic activity of peripheral blood neutrophils in goats. The results of the study indicate that the Migratest® kit can be used to evaluate the influence of immunomodulators on the chemotactic activity of peripheral blood neutrophils in goats. The results of the assay are most effectively presented by calculating the chemotactic index which accounts for the chemotaxis or migration of neutrophils in the presence or absence of a chemotactic factor, respectively, and the percentage of granulocytes that migrate towards fMLP. The results of both presentation methods appear to be identical.

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Evolution of Flow Cytometry Technology

Cited by 6 publications

References 14 publications

Extracellular Vesicles for the Diagnosis of Cancers

Extracellular Vesicles for the Diagnosis of Cancers

Unsupervised flow cytometry analysis in hematological malignancies: A new paradigm

Polish Journal of Veterinary Sciences

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