2016
DOI: 10.18632/oncotarget.12036
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Evolution of malignant plasmacytoma cell lines from K14E7 Fancd2−/− mouse long-term bone marrow cultures

Abstract: We tested the effect of expression of the Human Papilloma Virus (HPV E7) oncogene on hematopoiesis in long-term bone marrow cultures (LTBMCs) derived from K14E7 (FVB) Fancd2−/− (129/Sv), K14E7 Fancd2+/+, Fancd2−/−, and control (FVB X 129/Sv) Fl mice. K14E7 Fancd2−/− and Fancd2−/− LTBMCs showed decreased duration of production of total nonadherent hematopoietic cells and progenitors forming day 7 and day 14 multilineage CFU-GEMM colonies in secondary cultures (7 wks and 8 wks respectively) compared to cultures … Show more

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Cited by 3 publications
(16 citation statements)
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“…No carcinogenesis was demonstrated in control mouse strains, nor were squamous cell carcinomas detected in K14E6/7 Fancd2 −/− mice that were not exposed to 4-NQO or estrogen pellets (3). The question of whether HPV oncogenes were sufficient for malignant transformation was evaluated in a recent publication (1) in which continuous bone marrow culture from these K14E6/7 Fancd2 −/− mice, as well as Fancd2 −/− IL3-dependent hematopoietic progenitor cells maintained in the absence of a hematopoietic microenvironment, generated malignant plasmacytomainducing cell lines. While this model system tested the effect of HPV16 oncogenes in a different target organ (bone marrow), the system also provided the unique ability to study multilineage stem cell populations of the marrow in organ culture with an intact microenvironment.…”
Section: Discussionmentioning
confidence: 99%
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“…No carcinogenesis was demonstrated in control mouse strains, nor were squamous cell carcinomas detected in K14E6/7 Fancd2 −/− mice that were not exposed to 4-NQO or estrogen pellets (3). The question of whether HPV oncogenes were sufficient for malignant transformation was evaluated in a recent publication (1) in which continuous bone marrow culture from these K14E6/7 Fancd2 −/− mice, as well as Fancd2 −/− IL3-dependent hematopoietic progenitor cells maintained in the absence of a hematopoietic microenvironment, generated malignant plasmacytomainducing cell lines. While this model system tested the effect of HPV16 oncogenes in a different target organ (bone marrow), the system also provided the unique ability to study multilineage stem cell populations of the marrow in organ culture with an intact microenvironment.…”
Section: Discussionmentioning
confidence: 99%
“…Non-adherent cells were harvested from long-term cultures of bone marrow from each mouse genotype at week 4 and cultured in six-well tissue culture plates in Iscove's modified Eagle's medium (IMDM; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 20% fetal bovine serum (FBS) and 10 ng/ml recombinant mouse IL3 (Peprotech, Rocky Hill, NJ, USA). The cell lines were passaged weekly for 12-14 weeks to establish primary IL3-dependent cell lines using published methods (1).…”
mentioning
confidence: 99%
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