Evolution Toward Chip-Based Arrays in the Laboratory Diagnosis of Human Allergic Disease

Hamilton, Robert G.; Croote, Derek; Lupinek, Christian; Matsson, Per

doi:10.1016/j.jaip.2023.08.017

Cited by 7 publications

(1 citation statement)

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“…In addition to recognizing two different allergenic epitopes, another obvious advantage of mAb use is their consistent, reproducible quality (specificity and affinity) over time that does not require laborious reference reagent replacement. Indeed, we (DC & RG) have previously demonstrated the potential of human mAbs discovered from peanut-allergic individuals to serve as highly specific reagents in a diagnostic application ( 17 ).…”

Section: Discussionmentioning

confidence: 99%

A human monoclonal antibody based immunoenzymetric assay to measure Fel d 1 concentrations in cat hair and pelt allergenic extracts

Rabin,

Croote,

Chen

et al. 2024

Front. Allergy

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In the United States, 19 allergen extracts of different specificities are standardized, which means that their potencies are determined in comparison to a US reference standard. For cat allergen extracts, potency is determined by measuring Fel d 1 content expressed in in Fel d 1 units, and with a unitage that correlates with skin test reactions (bioequivalent allergy units or BAU). Currently, Fel d 1 content is measured with a radial immunodiffusion (RID) assay that uses polyclonal sheep antisera to detect the allergenic protein by producing a white precipitin line in agar gel. However, the RID is considered cumbersome, and the polyclonal sera may qualitatively vary among animals and may recognize epitopes irrelevant to human allergic disease. In this report, we describe a quantitative two-site immunoenzymetric assay (IEMA) for Fel d 1 that uses immobilized capture and soluble biotin-labeled detection Fel d 1-specific human IgE monoclonal antibodies (mAb) that have been class-switched to IgG4. Together, they sandwich Fel d 1 molecules from extracts. Using purified natural Fel d 1 as a calibrator, the historically reported ∼4 micrograms Fel d 1/Fel d 1 unit assignment was directly measured in this mAb-based IEMA at 3.12 ± 0.24 micrograms of Fel d 1 per Fel d 1 unit. This IEMA appears to be equivalent to RID in the measurement of biological potencies of commercial cat hair and cat pelt extracts marketed in the United States.

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Section: Discussionmentioning

confidence: 99%