Evolutionary and Characteristic Analysis of RING-DUF1117 E3 Ubiquitin Ligase Genes in Gossypium Discerning the Role of GhRDUF4D in Verticillium dahliae Resistance

Zhao, Yanpeng; Shen, Jian-Ling; Li, Wenjie; Wu, Na; Chen, Chen; Hou, Yuxia

doi:10.3390/biom11081145

Cited by 18 publications

(10 citation statements)

References 80 publications

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“…Several DUF family genes related to salt stress response have been reported. Overexpression of soybean GmCBSDUF3 , encoding DUF21, can enhance the tolerance of Arabidopsis thaliana to both drought and salt stress [ 42 ]. Overexpression of AhDGR2 (encoding a protein containing a DUF642 domain) in ‘Nipponbare’ resulted in increased sensitivity to NaCl treatment [ 43 ], OsSIDP366 (a gene containing DUF1644) positively regulates the response of rice to drought and salt stress [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of OsDUF6 increases salt stress tolerance in rice

Ma,

Zhang,

2024

BMC Plant Biol

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Background Soil salinity is one of the primary environmental stresses faced in rice production. When plants are exposed to salt stress, a series of cellular balances will be disrupted. Dufulin is an immune-induced antiviral agent used in plants. The DUF gene family influences plant response to abiotic stress, and the functional role of OsDUF6(ABA98726.1) in rice response to salt stress is being investigated here. Results Based on the transcriptome analysis of Dufulin treatment in inducing salt tolerance in rice, we selected the OsDUF6 protein located on the cell membrane and studied its molecular function by overexpressing OsDUF6. Salt-induced decreases in root, stem, and leaf length and increased leaf yellowing rate and Na+ concentration in the wild-type plant were mitigated in the overexpressed lines. OsDUF6 overexpression increased the enzymatic antioxidant activities of superoxide dismutase, peroxidase, catalase, and phenylalanine ammonia-lyase. OsDUF6 also played a positive role in Na+ transport as reflected by the increased growth of a salt-sensitive yeast mutant complemented with OsDUF6 in the presence of salt stress. In addition, Reverse transcription quantitative PCR analysis confirmed that the overexpression of OsDUF6 significantly changed the expression level of other genes related to growth and stress tolerance. Conclusions Combined with previously published data, our results supported the observation that OsDUF6 is an important functional factor in Dufulin-induced promotion of salt stress tolerance in rice.

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Section: Discussionmentioning

confidence: 99%

Overexpression of OsDUF6 increases salt stress tolerance in rice

Ma,

Zhang,

2024

BMC Plant Biol

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“…qGL3.5 encodes an IBR-RING zinc-finger protein with one RING and two IBR domains. Proteins containing zinc finger domains and RING-finger domains were found to play important roles in eukaryotic cells by regulating different signal transduction pathways and biological processes, such as plant growth development and resistance ( Yilmaza and Mittlera, 2008 ; Sun et al, 2019 ; Zhao et al, 2021 ). Duan et al (2020) reported a Cys2/His 2 zinc finger protein, ZFP207, that acts as a transcriptional repressor of OsGA20ox2 and negatively regulates plant height and GL in rice.…”

Section: Discussionmentioning

confidence: 99%

Identification of qGL3.5, a Novel Locus Controlling Grain Length in Rice Through Bulked Segregant Analysis and Fine Mapping

et al. 2022

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Grain length (GL) directly affects the yield and quality of rice. Very few cloned GL-related genes are applied in production because their yield-increasing effects are not obvious, and the overall regulatory networks underlying the associated processes remain poorly understood. DNA samples from two bulk DNA pools (L-pool and S-pool) and their parents (KJ01 and Huaye 3) were subjected to high-throughput sequencing. Using bulked segregant analysis (BSA), qGL3.5 was mapped to a 0.34-Mb “hotspot” region on chromosome 3 that contains 37 genes related to various traits. Then, qGL3.5 was mapped to the genomic interval between the flanking markers M2 and M3 using 2786 BC4F2 individuals. Because the region from B5 to B6 was not the associated region under BSA-seq analysis, qGL3.5 was narrowed down to the interval between B6 and M3, which spanned 24.0-kb. Of all 37 genes with non-synonymous single-nucleotide polymorphisms (SNPs) between KJ01 and Huaye 3 based on BSA-seq analysis, only one complete annotated gene, ORF18 (Gene ID: LOC_Os03g42790.1) was found. ORF18 encodes an IBR-RING zinc-finger-related protein, with one really interesting new gene (RING) and two in between ring finger (IBR) domains. The knockout of ORF18 derived from Huaye 3 using clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR-associated 9 (Cas9) editing technology increased the GL of the mutant by approximately 2.2 mm. The novel locus qGL3.5 negatively regulated GL by promoting elongation of the longitudinal cell of the grain outer glume. These results provide a new genetic resource for rice grain shape breeding and a starting point for the functional characterization of the wild rice GL gene.

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“…As in tomato, cotton plants are able to induce expression of NBS-LRR defence genes by suppressing the miR482-mediated gene silencing pathway upon fungal pathogen attack [ 80 ]. Particularly, miR482c might participate in cotton growth and abiotic stresses including drought stress response as a potential regulator [ 81 ]. As sequence variation induced target and mechanism variation, the function of members in miR482 family differ possibly from each other [ 78 , 82 ].…”

Section: Discussionmentioning

confidence: 99%

Novel insights into water-deficit-responsive mRNAs and lncRNAs during fiber development in Gossypium hirsutum

Yang

Wang

et al. 2022

BMC Plant Biol

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Background The fiber yield and quality of cotton are greatly and periodically affected by water deficit. However, the molecular mechanism of the water deficit response in cotton fiber cells has not been fully elucidated. Results In this study, water deficit caused a significant reduction in fiber length, strength, and elongation rate but a dramatic increase in micronaire value. To explore genome-wide transcriptional changes, fibers from cotton plants subjected to water deficit (WD) and normal irrigation (NI) during fiber development were analyzed by transcriptome sequencing. Analysis showed that 3427 mRNAs and 1021 long noncoding RNAs (lncRNAs) from fibers were differentially expressed between WD and NI plants. The maximum number of differentially expressed genes (DEGs) and lncRNAs (DERs) was identified in fibers at the secondary cell wall biosynthesis stage, suggesting that this is a critical period in response to water deficit. Twelve genes in cotton fiber were differentially and persistently expressed at ≥ five time points, suggesting that these genes are involved in both fiber development and the water-deficit response and could potentially be used in breeding to improve cotton resistance to drought stress. A total of 540 DEGs were predicted to be potentially regulated by DERs by analysis of coexpression and genomic colocation, accounting for approximately 15.76% of all DEGs. Four DERs, potentially acting as target mimics for microRNAs (miRNAs), indirectly regulated their corresponding DEGs in response to water deficit. Conclusions This work provides a comprehensive transcriptome analysis of fiber cells and a set of protein-coding genes and lncRNAs implicated in the cotton response to water deficit, significantly affecting fiber quality during the fiber development stage.

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Evolutionary and Characteristic Analysis of RING-DUF1117 E3 Ubiquitin Ligase Genes in Gossypium Discerning the Role of GhRDUF4D in Verticillium dahliae Resistance

Cited by 18 publications

References 80 publications

Overexpression of OsDUF6 increases salt stress tolerance in rice

Overexpression of OsDUF6 increases salt stress tolerance in rice

Identification of qGL3.5, a Novel Locus Controlling Grain Length in Rice Through Bulked Segregant Analysis and Fine Mapping

Novel insights into water-deficit-responsive mRNAs and lncRNAs during fiber development in Gossypium hirsutum

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