Evolutionary Comparison of the Developmental/Physiological Phenotype and the Molecular Behavior of SPIRRIG Between Arabidopsis thaliana and Arabis alpina

Stephan, Lisa; Jakoby, Marc; Hülskamp, Martin

doi:10.3389/fpls.2020.596065

Cited by 6 publications

(17 citation statements)

References 39 publications

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“…Two short root hair mutants had STOP codons in SPIRRIG (SPI) at positions 2,107 and 1976, similar to Atspi-4.2 (Saedler et al, 2009; Figure 4I; Supplementary Figure S1I). Four spi alleles in A. alpina with similar phenotypes have been previously reported (Chopra et al, 2019;Stephan et al, 2021). In four short root hair mutants, we found meaningful SNPs in the CAN OF WORMS 1 (COW1) gene (Grierson et al, 1997;Böhme et al, 2004;Figure 4J; Supplementary Figure S1J).…”

Section: Root Hair Morphology Mutantssupporting

confidence: 76%

Genetic and Molecular Analysis of Root Hair Development in Arabis alpina

et al. 2021

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Root hair formation in Arabidopsis thaliana is a well-established model system for epidermal patterning and morphogenesis in plants. Over the last decades, many underlying regulatory genes and well-established networks have been identified by thorough genetic and molecular analysis. In this study, we used a forward genetic approach to identify genes involved in root hair development in Arabis alpina, a related crucifer species that diverged from A. thaliana approximately 26–40 million years ago. We found all root hair mutant classes known in A. thaliana and identified orthologous regulatory genes by whole-genome or candidate gene sequencing. Our findings indicate that the gene-phenotype relationships regulating root hair development are largely conserved between A. thaliana and A. alpina. Concordantly, a detailed analysis of one mutant with multiple hairs originating from one cell suggested that a mutation in the SUPERCENTIPEDE1 (SCN1) gene is causal for the phenotype and that AaSCN1 is fully functional in A. thaliana. Interestingly, we also found differences in the regulation of root hair differentiation and morphogenesis between the species, and a subset of root hair mutants could not be explained by mutations in orthologs of known genes from A. thaliana. This analysis provides insight into the conservation and divergence of root hair regulation in the Brassicaceae.

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Section: Root Hair Morphology Mutantssupporting

confidence: 76%

Genetic and Molecular Analysis of Root Hair Development in Arabis alpina

et al. 2021

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“…This indicates that SPI has a dual role in membrane trafficking and RNA metabolism. Recently, the analysis of SPI in Arabis alpina has provided evidence that this dual role might be evolutionarily conserved (Stephan et al, 2021 ). These findings raise the question, whether other ESCRT proteins, or endosomal sorting in general, are associated with mRNA pathways.…”

Section: Introductionmentioning

confidence: 99%

Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective

et al. 2021

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The Arabidopsis AAA ATPase SKD1 is essential for ESCRT-dependent endosomal sorting by mediating the disassembly of the ESCRTIII complex in an ATP-dependent manner. In this study, we show that SKD1 localizes to messenger ribonucleoprotein complexes upon heat stress. Consistent with this, the interactome of SKD1 revealed differential interactions under normal and stress conditions and included membrane transport proteins as well as proteins associated with RNA metabolism. Localization studies with selected interactome proteins revealed that not only RNA associated proteins but also several ESCRTIII and membrane trafficking proteins were recruited to messenger ribonucleoprotein granules after heat stress.

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“…The BEACH domain proteins in eukaryotes cluster into four groups (A-D; Saedler et al, 2009) with similar domain structures (Supplementary Figure S1). AtSPI belongs to group A and is well conserved throughout the plant kingdom (Supplementary Figure S1; Stephan et al, 2021). Group A members share a Laminin G/Concanavalin A superfamily domain but lack additional domains found in the other groups.…”

Section: Resultsmentioning

confidence: 99%

“…Evolutionary comparison of SPI between A. thaliana and Arabis alpina demonstrated that the role of SPI in cell morphogenesis and endosomal pathways is conserved between these two Brassicaceae species (Stephan et al, 2021). This is evident from SPI co-localization and interaction with ESCRT components and a similar range of phenotypes.…”

Section: Introductionmentioning

confidence: 93%

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Functional analysis of the BEige and Chediak-Higashi domain gene MpSPIRRIG in Marchantia polymorpha

et al. 2022

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BEige and Chediak–Higashi domain containing proteins (BDCPs) have been described to function in membrane-dependent processes in eukaryotes. This role was also observed for the BDCP SPIRRIG (SPI) in the model plant Arabidopsis thaliana in the context of cell morphogenesis. Additionally, AtSPI was found to control salt stress resistance by mediating mRNA stability and salt stress-dependent processing body formation. In this work, we utilize an evolutionarily comparative approach to unravel conserved, basal BDCP functions in the liverwort Marchantia polymorpha. Our phenotypic and physiological analyses show that MpSPI is involved in cell morphogenesis and salt resistance regulation, indicating that both functions are evolutionarily conserved between the two species. Co-localization was found with endosomal and P-body markers, suggesting links to membrane-dependent processes and mRNA metabolism. Finally, we present transcriptomics data showing that AtSPI and MpSPI regulate orthologous genes in A. thaliana and M. polymorpha.

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Evolutionary Comparison of the Developmental/Physiological Phenotype and the Molecular Behavior of SPIRRIG Between Arabidopsis thaliana and Arabis alpina

Cited by 6 publications

References 39 publications

Genetic and Molecular Analysis of Root Hair Development in Arabis alpina

Genetic and Molecular Analysis of Root Hair Development in Arabis alpina

Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective

Functional analysis of the BEige and Chediak-Higashi domain gene MpSPIRRIG in Marchantia polymorpha

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