Interpretations of burial sites from past populations have previously relied on archaeological artifacts, body position, and gross morphological skeletal analysis to reconstruct funerary practices. Recently, microscopic analysis of biotic and abiotic inclusions in bone have resulted in broad assumptions about the antemortem treatment of human remains, including whether the bioerosion is of endogenous and/or exogenous origin. To contextualize the diagenesis present in bone, researchers have developed indices to quantify histotaphonomic features including overall destruction (OHI, GHI), birefringence (BI), cracking (CI), and color changes due to burning (HI). Quantification of the histotaphonomy of bone also allows researchers to determine if the bone is preserved well enough for the application of histological methods, such as age‐at‐death estimation, which can contribute to the osteobiography of the skeletal remains. However, burned bone found at cremation sites may complicate these analyses if thermal alterations obscure histological structures. Though many studies have experimentally tested the impact of burning on bone, most have used excised bones, which presents a very specific example of the effect of burning on excarnated remains. The aim of this research is to test the histotaphonomic effects of thermal alteration on six fleshed human bodies using the indices listed above. One preburn sample of bone from the femur, sixth rib, and metatarsal was collected prior to burning, and the antimere was removed after the experiment, if recoverable (N = 33). These results show that the presence of body tissue and the amount of time the body is burned likely have the greatest impact on bone histological preservation. None of the remains showed evidence of biotic bioerosion, which was expected from previous research that suggests putrefaction in the early postmortem period contributes to microfocal destruction that can be observed soon after death.
