Excavation, expression, and functional analysis of a novel zearalenone-degrading enzyme

Identification and Modification of Enzymatic Substrate Specificity through Residue Alteration in the Cap Domain: A Thermostable Zearalenone Lactonase

“…The M w of ZENM closely resembled that of other ZEN lactonases derived from G. roseum, 19 Trichoderma aggressivum, 20 E. spinifera, 17 F. monophora, 16 and Neurospora crassa. 21 It is worth noting that most ZEN lactonases have been identified as dimers, exemplified by CbZHD and ZHD101 from Cladophialophora bantiana 22 and C. rosea IFO 7063, 15 respectively.…”

“…aggressivum, and ZHD-LD from E. spinifera was found to range from 38 to 50 °C. Furthermore, ZENG lost approximately 50% of its initial activity following a 5 min incubation at 48 °C.…”

“…Unfortunately, most studied ZEN lactonases have demonstrated poor thermostability, posing a challenge in meeting the demands of industrial applications. For instance, the optimum temperature for ZENG from G. roseum, 19 ZHD-P from T. aggressivum, 20 and ZHD-LD from E. spinifera 17 was found to range from 38 to 50 °C. Furthermore, ZENG lost approximately 50% of its initial activity following a 5 min incubation at 48 °C.…”

“…Apart from microorganisms, enzymes have also proven to be efficient and eco-friendly biocatalysts for ZEN decontamination. , Lactonases play a crucial role in the feed industry for ZEN decontamination. , One such enzyme, lactonase ZHD101 from Clonostachys rosea IFO 7063, has shown remarkable capability in degrading ZEN . Additionally, lactonases from Fonsecaea monophora, Exophiala spinifera, and Rhinocladiella mackenziei have demonstrated the ability to degrade ZEN. However, the optimal temperature for these ZEN-degrading enzymes is below 50 °C, limiting their application in the feed industry.…”

“…13,14 One such enzyme, lactonase ZHD101 from Clonostachys rosea IFO 7063, has shown remarkable capability in degrading ZEN. 15 Additionally, lactonases from Fonsecaea monophora, 16 Exophiala spinifera, 17 and Rhinocladiella mackenziei have demonstrated the ability to degrade ZEN. However, the optimal temperature for these ZEN-degrading enzymes is below 50 °C, limiting their application in the feed industry.…”