Excellence of the two-layer method (University of Wisconsin solution/perfluorochemical) in pancreas preservation before islet isolation

Tanioka, Yasuki; Sutherland, David E.R.; Kuroda, Yoshikazu; Gilmore, Tom; Asaheim, Tor C; Kronson, Jeffrey W.; Leone, John P.

doi:10.1016/s0039-6060(97)90037-4

Cited by 64 publications

(41 citation statements)

References 22 publications

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“…Researchers at the University of Minnesota have demonstrated in experimental animal models and research human pancreata that the TLM can resuscitate and repair warm ischemically-damaged pancreata during preservation, improve islet yields, and improve islet engraftment [215,216,217,218,219,220,2211. TLM also maintains and repairs exocrine cell integrity and prevents trypsin activation, thereby enabling effective collagenase delivery and protecting the islets from enzymatic digestion [222].…”

Section: Oxygenated Pfc-based Preservationmentioning

confidence: 99%

Technical aspects of islet preparation and transplantation

Lakey

Burridge

Shapiro

2003

Transplant International

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The introduction of insulin therapy for the management of diabetes mellitus is arguably the greatest milestone in the history of modern medicine. P-cell replacement therapy is the only treatment that reestablishes and maintains longterm physiological normoglycemia. Until recently, successful clinical outcomes of pancreas transplantation for patients with long-standing diabetes were much superior to that of islet transplantation. Significant advances in islet isolation and purification technology, the development of more specific and less diabetogenic immunosuppressants and the prophylactic administration of antiviral agents have rekindled a worldwide interest in islet transplantation. This chapter will review the rationale of islet transplantation and the development of islet isolation and purification. The challenges facing clinical islet transplantation in the twenty-first century will also be introduced.

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Section: Oxygenated Pfc-based Preservationmentioning

confidence: 99%

Technical aspects of islet preparation and transplantation

Lakey

Burridge

Shapiro

2003

Transplant International

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“…Consequently, the TLM was reported to achieve better organ function in experimental pancreas [5,6] , heart [7] and intestinal transplantation [8] and to rescue the graft in a model of prolonged warm ischemia time [9] . Higher pancreatic islet yield and better function has been found by several research groups in comparison with conventional UW pancreas storage in experimental [10][11][12] , as well as in clinical studies [13,14] , though this effect has not been confirmed in a larger series [15][16][17] . The TLM for pancreas transplantation has therefore become a standard procedure in several centers supposedly enabling to prolong the cold ischemia time before islet isolation [18][19][20][21] .…”

Section: Introductionmentioning

confidence: 94%

Perfluorocarbon Improves Post-Transplant Survival and Early Kidney Function following Prolonged Cold Ischemia

Marada

Zacharovová²,

Saudek³

2010

Eur Surg Res

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Background: The two-layer organ preservation method (TLM) based on oxygenated perfluorocarbon overlaid with University of Wisconsin (UW) solution has been successfully used in clinical islet and experimental heart and intestine transplantation. We tested whether this technique would prevent tissue damage and improve kidney function in a model of syngeneic kidney transplantation with prolonged ischemia time. Methods: Kidneys were stored for 24 h either in UW solution (n = 16), with TLM (n = 16) or transplanted immediately (control group, n = 12). In half of the animals, survival was observed and in the other animals grafts were procured for semiquantitative histological scoring and TUNEL apoptosis assessment 24 h after transplantation. Results: One-month survival rates in the UW, TLM and control groups were 12.5, 62.5 and 100%, respectively (UW vs. TLM, p < 0.01). Median creatinine levels 24 h after transplantation were 381, 299 and 121 µM, respectively (UW vs. TLM, p < 0.02). Histological scoring showed more severe tissue damage in the UW group than in the TLM group (p < 0.05). Apoptosis was more frequent in the UW group than in the TLM group (p < 0.05). Conclusion: We demonstrated for the first time that conservation with TLM significantly improves the outcome of kidney transplantation in a rat model and should therefore be further studied in larger animals.

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“…It was shown that significantly more islets can be isolated utilizing TLM during prolonged cold storage compared to UWS. However, these experiments are difficult to compare with subsequent studies in human pancreases, since retrieved canine pancreases were not perfused with organ preservation solution prior to cold storage (Tanioka et al, 1997b). The setting for first studies in human pancreases was different to subsequent ones because of logistical reasons.…”

Section: Pancreas Oxygenation For Subsequent Islet Transplantationmentioning

confidence: 99%

Chemical Oxygenation of Pancreatic Tissue Prior to Islet Isolation and Transplantation

Brandhorst¹,

Johnson²,

Brandhorst³

2012

Organ Donation and Transplantation - Public Policy and Clinical Perspectives

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Excellence of the two-layer method (University of Wisconsin solution/perfluorochemical) in pancreas preservation before islet isolation

Cited by 64 publications

References 22 publications

Technical aspects of islet preparation and transplantation

Technical aspects of islet preparation and transplantation

Perfluorocarbon Improves Post-Transplant Survival and Early Kidney Function following Prolonged Cold Ischemia

Chemical Oxygenation of Pancreatic Tissue Prior to Islet Isolation and Transplantation

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