Excellent physical durability and enhanced Fano absorption of SPR sensor platform based on Au-covered silica sphere monolayer

Choi, Bok Hee; Park, Dae Keun; Leo, Sin‐Yen; Jiang, Peng

doi:10.1007/s11814-023-1393-3

Cited by 1 publication

(1 citation statement)

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“…The sensitivity was improved with the addition of the binding, according to the transition from the former to the latter mode. The addition was acquired mainly through nanostructures including magnetic particles, gold nanomaterials, quantum dots, and graphene [ 7 , 10 , 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Surface-Plasmon-Resonance Amplification of FMD Detection through Dendrimer Conjugation

Jung,

Park

2024

Sensors

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The amplification of the surface plasmon resonance (SPR) sensitivity for the foot-and-mouth disease (FMD) detection was studied using Poly(amidoamine) (PAMAM) succinamic-acid dendrimers. The dendrimers were conjugated with the complementary annealed with the aptamers capable of binding specifically to FMD peptides. The tethered layer of the dendrimer-conjugated double-stranded(ds)-aptamers was formed on the SPR sensor Au surface via a thiol bond between the aptamers and Au. After the tethered layer was formed, the surface was taken out of the SPR equipment. Then, the ds-aptamers on the surface were denatured to collect the dendrimer-conjugated single-stranded(ss)-complementary. The surface with only the remaining ss-aptamers was transferred again to the equipment. Two types of the injections, the FMD peptide only and the dendrimer-conjugated ss-complementary followed by the FMD peptides, were performed on the surface. The sensitivity was increased 20 times with the conjugation of the dendrimers, but the binding rate of the peptides became more than two times slower.

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