Excess glucose or fat differentially affects metabolism and appetite-related gene expression during zebrafish embryogenesis

Konadu, Bridget; Cox, Carol K.; Garrett, Michael R.; Gibert, Yann

doi:10.1016/j.isci.2023.107063

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2024

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Cited by 2 publications

(1 citation statement)

References 90 publications

(108 reference statements)

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“… Timing: 1 h Note: In our paper, 1 we performed the injection of BODIPY and TAG at 24 hpf. BODIPY injection can basically take place at any time during zebrafish development.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Protocol for the microinjection of free fatty acids and triacylglycerol in zebrafish embryos

Konadu,

Cox,

Gibert

2024

STAR Protocols

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“… Timing: 1 h Note: In our paper, 1 we performed the injection of BODIPY and TAG at 24 hpf. BODIPY injection can basically take place at any time during zebrafish development.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Protocol for the microinjection of free fatty acids and triacylglycerol in zebrafish embryos

Konadu,

Cox,

Gibert

2024

STAR Protocols

Self Cite

View full text Add to dashboard Cite

Oxidative Stress, Inflammation and Altered Glucose Metabolism Contribute to the Retinal Phenotype in the Choroideremia Zebrafish

Méjécase,

Nair,

Sarkar

et al. 2024

Antioxidants

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Reactive oxygen species (ROS) within the retina play a key role in maintaining function and cell survival. However, excessive ROS can lead to oxidative stress, inducing dysregulation of metabolic and inflammatory pathways. The chmru848 zebrafish models choroideremia (CHM), an X-linked chorioretinal dystrophy, which predominantly affects the photoreceptors, retinal pigment epithelium (RPE), and choroid. In this study, we examined the transcriptomic signature of the chmru848 zebrafish retina to reveal the upregulation of cytokine pathways and glia migration, upregulation of oxidative, ER stress and apoptosis markers, and the dysregulation of glucose metabolism with the downregulation of glycolysis and the upregulation of the oxidative phase of the pentose phosphate pathway. Glucose uptake was impaired in the chmru848 retina using the 2-NBDG glucose uptake assay. Following the overexpression of human PFKM, partial rescue was seen with the preservation of photoreceptors and RPE and increased glucose uptake, but without modifying glycolysis and oxidative stress markers. Therapies targeting glucose metabolism in CHM may represent a potential remedial approach.

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Excess glucose or fat differentially affects metabolism and appetite-related gene expression during zebrafish embryogenesis

Cited by 2 publications

References 90 publications

Protocol for the microinjection of free fatty acids and triacylglycerol in zebrafish embryos

Protocol for the microinjection of free fatty acids and triacylglycerol in zebrafish embryos

Oxidative Stress, Inflammation and Altered Glucose Metabolism Contribute to the Retinal Phenotype in the Choroideremia Zebrafish

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