Excess Imidacloprid Exposure Causes the Heart Tube Malformation of Chick Embryos

Gao, Lin; Li, Shuai; Zhang, Jing; Liang, Chang; Chen, En-ni; Zhang, Shiyao; Chuai, Manli; Bao, Yongping; Wang, Guang; Yang, Xuesong

doi:10.1021/acs.jafc.6b03381

Cited by 18 publications

(15 citation statements)

References 52 publications

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“…Additionally, high‐salt destroyed the cytoskeleton and enhanced cell junctions (Figures l and n), inhibited cell migration and specification. Thus, we found the excessive ectopic expression of E‐cadherin and N‐cadherin, which indicated abnormal mechanism during the EMT process of heart tube formation (Gao et al, ) (Supplementary Figure S2).…”

Section: Discussionmentioning

confidence: 83%

“…A “scratch wound” was created by scraping a monolayer culture of H9c2 cells using a sterile 10 µl pipet tip and the length of the wound gap was measured using Image pro‐Plus software as described previously (Cheng et al, ; Gao et al, ). The assays were performed three times using triplicate culture wells.…”

Section: Methodsmentioning

confidence: 99%

“…Chick embryos were harvested and fixed in 4% paraformaldehyde solution overnight at 4 °C. The fixed embryos were implemented according to a standard protocol (Gao et al, ). Whole‐mount embryos were performed using the following primary antibodies: MF20 (1:500, DSHB, Iowa, IA), E‐cadherin (1:50, BD Transduction Laboratories, Franklin Lake, NJ), N‐cadherin (1:50, DSHB), Laminin (1:100, DSHB), pHIS3 (p‐histone H3, 1:400, Santa Cruz, Santa Cruz, CA), and C‐caspase3 (Cleaved Capase‐3, 1:200, Cell Signaling, Danvers, MA).…”

Section: Methodsmentioning

confidence: 99%

“…For Hochest/PI staining, the cells were cultured and washed twice with cold PBS, and then incubated with Hochest/PI for 45 min at 37 °C in the dark. The photography and cryostat microtome were performed as previous reported by Gao et al () and Wang et al ().…”

Section: Methodsmentioning

confidence: 99%

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High salt‐induced excess reactive oxygen species production resulted in heart tube malformation during gastrulation

Gao¹,

Wang²,

Zhang³

et al. 2018

Journal Cellular Physiology

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An association has been proved between high salt consumption and cardiovascular mortality. In vertebrates, the heart is the first functional organ to be formed. However, it is not clear whether high-salt exposure has an adverse impact on cardiogenesis. Here we report high-salt exposure inhibited basement membrane breakdown by affecting RhoA, thus disturbing the expression of Slug/E-cadherin/N-cadherin/Laminin and interfering with mesoderm formation during the epithelial-mesenchymal transition(EMT). Furthermore, the DiI cell migration trajectory in vivo and scratch wound assays in vitro indicated that high-salt exposure restricted cell migration of cardiac progenitors, which was caused by the weaker cytoskeleton structure and unaltered corresponding adhesion junctions at HH7. Besides, down-regulation of GATA4/5/6, Nkx2.5, TBX5, and Mef2c and up-regulation of Wnt3a/β-catenin caused aberrant cardiomyocyte differentiation at HH7 and HH10. High-salt exposure also inhibited cell proliferation and promoted apoptosis. Most importantly, our study revealed that excessive reactive oxygen species(ROS)generated by high salt disturbed the expression of cardiac-related genes, detrimentally affecting the above process including EMT, cell migration, differentiation, cell proliferation and apoptosis, which is the major cause of malformation of heart tubes.

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Section: Discussionmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

High salt‐induced excess reactive oxygen species production resulted in heart tube malformation during gastrulation

Gao¹,

Wang²,

Zhang³

et al. 2018

Journal Cellular Physiology

Self Cite

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“…The expression of acetylcholine receptor (AChR) and acetylcholinesterase (AChE) was found in NCCs, suggesting that both might play role in the NCCs development (Cochard & Coltey, ; Howard, Gershon, & Margiotta, ). Finally, exposure of the early chick embryo to imidacloprid caused an inhibition of cardiomyocyte differentiation and malformation of the heart tube, which is partly derived from the cardiac NC (Gao et al, ) and also is regulated by these cells (Evans et al, ). This suggests that this chemical agent is an important teratogen in NC development.…”

Section: Chemical Agentsmentioning

confidence: 99%

The role of teratogens in neural crest development

Cerrizuela

Vega-Lopez

Aybar

2020

Birth Defects Research

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The neural crest (NC), discovered by Wilhelm His 150 years ago, gives rise to a multipotent migratory embryonic cell population that generates a remarkably diverse and important array of cell types during the development of the vertebrate embryo. These cells originate in the neural plate border (NPB), which is the ectoderm between the neural plate and the epidermis. They give rise to the neurons and glia of the peripheral nervous system, melanocytes, chondrocytes, smooth muscle cells, odontoblasts and neuroendocrine cells, among others. Neurocristopathies are a class of congenital diseases resulting from the abnormal induction, specification, migration, differentiation or death of NC cells (NCCs) during embryonic development and have an important medical and societal impact. In general, congenital defects affect an appreciable percentage of newborns worldwide. Some of these defects are caused by teratogens, which are agents that negatively impact the formation of tissues and organs during development. In this review, we will discuss the teratogens linked to the development of many birth defects, with a strong focus on those that specifically affect the development of the NC, thereby producing neurocristopathies.Although increasing attention is being paid to the effect of teratogens on embryonic development in general, there is a strong need to critically evaluate the specific role of these agents in NC development. Therefore, increased understanding of the role of these factors in NC development will contribute to the planning of strategies aimed at the prevention and treatment of human neurocristopathies, whose etiology was previously not considered.

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An update of the Worldwide Integrated Assessment (WIA) on systemic insecticides. Part 2: impacts on organisms and ecosystems

Pisa

Goulson

Yang

et al. 2017

Environ Sci Pollut Res

202

144

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New information on the lethal and sublethal effects of neonicotinoids and fipronil on organisms is presented in this review, complementing the previous Worldwide Integrated Assessment (WIA) in 2015. The high toxicity of these systemic insecticides to invertebrates has been confirmed and expanded to include more species and compounds. Most of the recent research has focused on bees and the sublethal and ecological impacts these insecticides have on pollinators. Toxic effects on other invertebrate taxa also covered predatory and parasitoid natural enemies and aquatic arthropods. Little new information has been gathered on soil organisms. The impact on marine and coastal ecosystems is still largely uncharted. The chronic lethality of neonicotinoids to insects and crustaceans, and the strengthened evidence that these chemicals also impair the immune system and reproduction, highlights the dangers of this particular insecticidal class (neonicotinoids and fipronil), with the potential to greatly decrease populations of arthropods in both terrestrial and aquatic environments. Sublethal effects on fish, reptiles, frogs, birds, and mammals are also reported, showing a better understanding of the mechanisms of toxicity of these insecticides in vertebrates and their deleterious impacts on growth, reproduction, and neurobehaviour of most of the species tested. This review concludes with a summary of impacts on the ecosystem services and functioning, particularly on pollination, soil biota, and aquatic invertebrate communities, thus reinforcing the previous WIA conclusions .

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Excess Imidacloprid Exposure Causes the Heart Tube Malformation of Chick Embryos

Cited by 18 publications

References 52 publications

High salt‐induced excess reactive oxygen species production resulted in heart tube malformation during gastrulation

High salt‐induced excess reactive oxygen species production resulted in heart tube malformation during gastrulation

The role of teratogens in neural crest development

An update of the Worldwide Integrated Assessment (WIA) on systemic insecticides. Part 2: impacts on organisms and ecosystems

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