Exclusion of sequence polymorphisms in the porcine <i>ITGA5</i> and <i>MIR148B</i> loci as causal variation for congenital splay leg in piglets

Maak, Steffen; Boettcher, Diana; Komolka, Katrin; Tetens, Jens; Wimmers, Klaus; Reinsch, Norbert; Swalve, Hermann H.; Thaller, Georg

doi:10.1111/j.1365-2052.2010.02047.x

Cited by 10 publications

(6 citation statements)

References 6 publications

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“…The miR-148b gene has two SNPs, one of which alters a potential miRNA core promoter sequence. This SNP has different frequencies between different breeds of pig, but is not associated with splay leg condition (Maak et al, 2010). One T/C SNP in miR-27a gene, detected in Large White and Dam line of Chinese lean-type new lines (DIV) pigs, is associated with litter size (Lei et al, 2011).…”

Section: Mirnas In Pigsmentioning

confidence: 99%

MicroRNAs in farm animals

Wang

Jiang

2013

Animal

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MicroRNAs (miRNAs) are a class of ,22 nucleotide-long small noncoding RNAs that target mRNAs for translational repression or degradation. miRNAs target mRNAs by base-pairing with the 3 0 -untranslated regions (3 0 -UTRs) of mRNAs. miRNAs are present in various species, from animals to plants. In this review, we summarize the identification, expression, and function of miRNAs in four important farm animal species: cattle, chicken, pig and sheep. In each of these species, hundreds of miRNAs have been identified through homology search, small RNA cloning and next generation sequencing. Real-time RT-PCR and microarray experiments reveal that many miRNAs are expressed in a tissue-specific or spatiotemporal-specific manner in farm animals. Limited functional studies suggest that miRNAs have important roles in muscle development and hypertrophy, adipose tissue growth, oocyte maturation and early embryonic development in farm animals. Increasing evidence suggests that single-nucleotide polymorphisms in miRNA target sites or miRNA gene promoters may contribute to variation in production or health traits in farm animals.

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Section: Mirnas In Pigsmentioning

confidence: 99%

MicroRNAs in farm animals

Wang

Jiang

2013

Animal

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“…This is attributed to hypomyelination of the spinal cord and the nerves innervating the affected muscles [ 23 , 24 ]. The syndrome was first described in 1967 [ 23 ] and the hitherto proposed causal factors are numerous e.g., heritable gene defects, viral infections, various management factors, nutrition, and, fusarium toxin in the feed of the pregnant dam [ 21 , 25 – 27 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of atypical porcine pestivirus in Swedish piglets with congenital tremor type A-II

2020

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Background: Congenital tremor (CT) type A-II is a neurological disorder characterized by tremor of the head and body of newborn piglets. The suggested causative agent of the disease is the recently found atypical porcine pestivirus (APPV). The virus has been detected in piglets suffering from congenital tremor in central Europe, South and North America and in China but no studies has so far been performed in the Nordic countries. The overarching goal of this study was to investigate if APPV is present in the brain tissue of Swedish piglets suffering from congenital tremor. From June 2017-June 2018, 15 piglets from four Swedish farms with ongoing outbreaks of congenital tremor and 13 piglets with splay leg originating from four different farms, were investigated for presence of APPV RNA in brain tissue. Matched healthy control piglets (n = 8) were also investigated. Two APPV-specific RT-qPCR methods targeting the NS3 and NS5B region, respectively, were used. A retrospective study was performed on material from Swedish piglets with congenital tremor sampled in 2004 (n = 11) and 2011/2012 (n = 3) using the described APPVspecific RT-qPCR methods. The total number of piglets with signs of CT in this study was 29. Results: Atypical porcine pestivirus-RNA was detected in 93% (27/29) of the piglets suffering from congenital tremor. All piglets with congenital tremor from 2004 (n = 11) and 2012 (n = 3) were PCR-positive with respect to APPV, whereas, all of the healthy controls (n = 11) were negative. The piglets with congenital tremor sampled 2017-2018 had an odds ratio of 91.8 (95% CI 3.9128 to 2153.7842, z = 2.807, P = 0.0050) to test positive for APPV by qRT-PCR compared to the healthy piglets (Fishers exact test p < 0.0001). These findings make it interesting to continue investigating APPV in the Swedish pig-population. Conclusion: This is the first description of atypical porcine pestivirus in piglets suffering from congenital tremor type A-II in Sweden and the Nordic countries. The virus has been present in the Swedish pig population since at least 2004.

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“…However no significant differences were found using a standard paired t-test, only four genes ( SQSTM1, SSRP1, DDIT4, MAF ) with significant (Wilcoxon, p < 0.05) differential expression levels in at least two muscles were found after using a sum rank test and suggested further investigation. In their following study [14, 15], another two differentially expressed gene ITGA5 , ZDHHC9 were also excluded as candidate genes for PSL.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide association study identifies candidate genes for piglet splay leg syndrome in different populations

Hao

Plastow

et al. 2017

BMC Genet

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BackgroundPiglet splay leg syndrome (PSL) is one of the most frequent genetic defects, and can cause considerable economic loss in pig production. The present understanding of etiology and pathogenesis of PSL is poor. The current study focused on identifying loci associated with PSL through a genome-wide association study (GWAS) performed with the Illumina Porcine60 SNP Beadchip v2.0. The study was a case/control design with four pig populations (Duroc, Landrace, Yorkshire and one crossbred of Landrace × Yorkshire).ResultAfter quality control of the genotyping data, 185 animals (73 cases, 112 controls) and 43,495 SNPs were retained for further analysis. Principal components (PCs) identified from the genomic kinship matrix were included in the statistical model for correcting the effect of population structure. Seven chromosome-wide significant SNPs were identified on Sus scrofa chromosome 1 (SSC1), SSC2 (2 SNPs), SSC7, SSC15 (2 SNPs) and SSC16 after strict Bonferroni correction. Four genes (HOMER1 and JMY on SSC2, ITGA1 on SSC16, and RAB32 on SSC1) related to muscle development, glycogen metabolism and mitochondrial dynamics were identified as potential candidate genes for PSL.ConclusionsWe identified seven chromosome-wide significant SNPs associated with PSL and four potential candidate genes for PSL. To our knowledge, this is the first pilot study aiming to identify the loci associated with PSL using GWAS. Further investigations and validations for those findings are encouraged.

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Exclusion of sequence polymorphisms in the porcine ITGA5 and MIR148B loci as causal variation for congenital splay leg in piglets

Cited by 10 publications

References 6 publications

MicroRNAs in farm animals

MicroRNAs in farm animals

Detection of atypical porcine pestivirus in Swedish piglets with congenital tremor type A-II

Genome-wide association study identifies candidate genes for piglet splay leg syndrome in different populations

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