Exercise coupled with calorie restriction reduces SIRT1 and SIRT2 in female obese adolescents

Abstract

Background: As exercise coupled with calorie restriction was a most effective method for long-term weight control, whether exercise coupled with calorie restriction controls the expression of SIRT1 and SIRT2 and how the mechanisms work become important. Long-term benefits of conservative treatments in obese adolescents are limited, except for exercise training. Exercise training coupled with calorie restriction as a most effective method for long-term weight control has become increasingly considerable. Methods: Statistical significance for serum biomarkers associated with fat etabolism and longevity measured before and after intervention was evaluated using a paired sample t-test. Correlation coefficients were used to examine the relationships between fat metabolism and longevity biomarkers and physical composition parameters. Results: Physical composition parameters revealed a significant decline after 4-week intervention involving in exercise training coupled with calorie restriction(p < 0.05). Exercise coupled with dietary restriction decreases circulating serum SIRT1 and SIRT2 levels(p< 0.05). Circulating serum SIRT2 levels negatively correlate with vital capacity before 4-week intervention(p < 0.05). Circulating serum IGF1/IGFBP3 levels positively correlate with vital capacity after 4-week intervention(p < 0.05). Conclusions: 4-week exercise training and calorie restriction may upregulate Sirt1 gene expression in nucleus and downregulate mRNA translation in cytoplasm,which in turn, reduced circulating Sirt1 and Sirt2.