Exercise training induces insulin-sensitizing PAHSAs in adipose tissue of elderly women

Brezinova, Marie; Čajka, Tomáš; Oseeva, Marina; Štěpán, Marek; Daďová, Klára; Rossmeislová, Lenka; Matouš, Miloš; Šiklová, Michaela; Rossmeisl, Martin; Kuda, Ondřej

doi:10.1016/j.bbalip.2019.158576

Cited by 35 publications

(46 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, the objective of the EXODYA project, which our investigation is a part of, is to study metabolic health using sophisticated biochemical and metabolomic methods. In recently published article we have shown positive effects of this intervention on metabolic parameters [30].…”

Section: Discussionmentioning

confidence: 85%

Effect of Calanus Oil Supplementation and 16 Week Exercise Program on Selected Fitness Parameters in Older Women

et al. 2020

View full text Add to dashboard Cite

We investigated changes in functional fitness after an exercise program in combination with Calanus oil supplementation, a novel source of bioactive lipids rich in wax esters with omega-3 polyunsaturated fatty acid (n-3 PUFA). Fifty-five healthy sedentary women aged 65–80 (mean age 70.9 ± 3.9 years, BMI 27.24 ± 3.9 kg m−2, VO2peak 19.46 ± 3.7 ml kg−1 min−1) were enrolled in the study. The participants were divided into two groups: exercise training plus Calanus Oil supplementation (n = 28) or exercise plus placebo (sunflower oil) supplementation (n = 27). The exercise intervention program was completed by 53 participants and contained functional circuit training (twice a week, 45 min plus 15 min of stretching and balance training) and Nordic walking (once a week, 60 min) for 16 weeks. Senior fitness test, exercise stress test on bicycle ergometer, hand-grip, and body composition were evaluated before and after the program. Our results show that functional fitness and body composition improved following the interventional exercise program, but for most of the parameters there was no synergic effect of supplementing n-3 PUFA-rich Calanus oil. In comparison to the placebo group, the group with Calanus supplementation experienced significantly higher improvement of functional strength of lower body which was evaluated by the chair stand test. Supplementation with Calanus may have a synergic effect with exercise on functional strength of the lower body in the elderly.

show abstract

Section: Discussionmentioning

confidence: 85%

Effect of Calanus Oil Supplementation and 16 Week Exercise Program on Selected Fitness Parameters in Older Women

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Both animals and plants synthesize FAHFAs, but the biosynthetic pathways are largely unknown. FAHFA stereochemistry, branching position, acyl composition, concentration and FAHFA‐containing complex lipids such as TAG estolides or digalactosyldiglycerols [ 23,38 ] define a huge matrix of combinations allowing multiple synthetic and degradation pathways to act simultaneously. So far, the most complex FAHFA‐ome has been found in subcutaneous WAT of mice where multiple positional isomers were detected.…”

Section: Discussionmentioning

confidence: 99%

“…[ 19 ] If we consider that the inverse combinations of DHAHLA and EPAHLA, specifically LAHDHAs and LAHEPAs (linoleic acid esters of hydroxy DHA and hydroxy EPA, respectively) were not detected in this experiment (not shown) and that these FAHFAs are very rare, [ 2,39 ] we can speculate that omega‐3 PUFAs serve mainly as the acyl building blocks and not as the hydroxy fatty acid backbones. Of note, the TAG‐bound FAHFA metabolism was recently described, [ 9,23,38 ] highlighting the role of TAG lipid class in FAHFA synthesis. Altogether, we could speculate that the TAG species are important for the availability of a fatty acid for hydroxy fatty acid esterification.…”

Section: Discussionmentioning

confidence: 99%

“…Extraction of metabolites from 20 mg of oil was carried out using a biphasic solvent system of cold methanol, methyl tert ‐butyl ether, and water with some modifications. [ 22,23 ] An aliquot of the lipid extract was processed via lipidomic pipeline while another was used for FAHFA analysis. The LC–MS system consisted of a Vanquish UHPLC System (Thermo Fisher Scientific, Bremen, Germany) coupled to a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

“…The residues were dissolved in 100 µL of dichloromethane/isopropanol/methanol, 1:4:2, vortexed, centrifuged (16 000 × g , 10 min, 4 °C) and transferred to a vial. Free fatty acids were analyzed using LC–MS as before [ 22,23 ] and data expressed as percentage of total fatty acids per sample.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Triacylglycerol‐Rich Oils of Marine Origin are Optimal Nutrients for Induction of Polyunsaturated Docosahexaenoic Acid Ester of Hydroxy Linoleic Acid (13‐DHAHLA) with Anti‐Inflammatory Properties in Mice

Paluchová

Vik

Čajka

et al. 2020

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

Scope The docosahexaenoic acid ester of hydroxy linoleic acid (13‐DHAHLA) is a bioactive lipid with anti‐inflammatory properties from the family of fatty acid esters of hydroxy fatty acids (FAHFA). Methods and results To explore the biosynthesis of 13‐DHAHLA from dietary oils, C57BL/6N mice are gavaged for 8 days with various corn oil/marine oil mixtures containing the same amount of DHA. Plasma levels of omega‐3 FAHFAs are influenced by the lipid composition of the mixtures but do not reflect the changes in bioavailability of polyunsaturated fatty acids in plasma. Triacylglycerol‐bound DHA and linoleic acid serve as more effective precursors for 13‐DHAHLA synthesis than DHA bound in phospholipids or wax esters. Both 13(S)‐ and 13(R)‐DHAHLA inhibit antigen and PGE2‐induced chemotaxis and degranulation of mast cells to a comparable extent and 13(S)‐DHAHLA is identified as the predominant isomer in mouse adipose tissue. Conclusion Here, the optimal nutritional source of DHA is identified, which supports production of anti‐inflammatory FAHFAs, as triacylglycerol‐based marine oil and also reveals a possible role of triacylglycerols in the synthesis of FAHFA lipokines.

show abstract

The role of adipogenic capacity and dysfunctional subcutaneous adipose tissue in the inheritance of type 2 diabetes mellitus: cross‐sectional study

Šiklová,

Šrámková,

Koc

et al. 2024

Obesity

Self Cite

View full text Add to dashboard Cite

ObjectiveThis study tested the hypothesis that limited subcutaneous adipose tissue (SAT) expansion represents a primary predisposition to the development of type 2 diabetes mellitus (T2DM), independent of obesity, and identified novel markers of SAT dysfunction in the inheritance of T2DM.MethodsFirst‐degree relatives (FDR) of T2DM patients (n = 19) and control individuals (n = 19) without obesity (fat mass < 25%) were cross‐sectionally compared. Body composition (bioimpedance, computed tomography) and insulin sensitivity (IS; oral glucose tolerance test, clamp) were measured. SAT obtained by needle biopsy was used to analyze adipocyte size, lipidome, mRNA expression, and inflammatory markers. Primary cultures of adipose precursors were analyzed for adipogenic capacity and metabolism.ResultsCompared with control individuals, FDR individuals had lower IS and a higher amount of visceral fat. However, SAT‐derived adipose precursors did not differ in their ability to proliferate and differentiate or in metabolic parameters (lipolysis, mitochondrial oxidation). In SAT of FDR individuals, lipidomic and mRNA expression analysis revealed accumulation of triglycerides containing polyunsaturated fatty acids and increased mRNA expression of lysyl oxidase (LOX). These parameters correlated with IS, visceral fat accumulation, and mRNA expression of inflammatory and cellular stress genes.ConclusionsThe intrinsic adipogenic potential of SAT is not affected by a family history of T2DM. However, alterations in LOX mRNA and polyunsaturated fatty acids in triacylglycerols are likely related to the risk of developing T2DM independent of obesity.image

show abstract

Exercise training induces insulin-sensitizing PAHSAs in adipose tissue of elderly women

Cited by 35 publications

References 40 publications

Effect of Calanus Oil Supplementation and 16 Week Exercise Program on Selected Fitness Parameters in Older Women

Effect of Calanus Oil Supplementation and 16 Week Exercise Program on Selected Fitness Parameters in Older Women

Triacylglycerol‐Rich Oils of Marine Origin are Optimal Nutrients for Induction of Polyunsaturated Docosahexaenoic Acid Ester of Hydroxy Linoleic Acid (13‐DHAHLA) with Anti‐Inflammatory Properties in Mice

The role of adipogenic capacity and dysfunctional subcutaneous adipose tissue in the inheritance of type 2 diabetes mellitus: cross‐sectional study

Contact Info

Product

Resources

About