Exhaled nitric oxide in COPD: glancing through a smoke screen

Sterk, Peter J.; Gouw, H. W. De; Ricciardolo, Fabio Luigi Massimo; Rabe, Klaus F.

doi:10.1136/thx.54.7.565

Cited by 31 publications

(16 citation statements)

References 72 publications

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“…Several groups of investigators have attempted to describe eNO values in COPD previously with conflicting results [12]. There are many possible explanations for such inconsistant results.…”

Section: Discussionmentioning

confidence: 99%

Exhaled nitric oxide in chronic obstructive pulmonary disease: relationship to pulmonary function

Ansarin¹,

Chatkin²,

Ferreira³

et al. 2001

Eur Respir J

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The following study was undertaken in order to determine how exhaled nitric oxide (eNO) levels in former smokers with chronic obstructive pulmonary disease (COPD) compared to eNO levels in patients with asthma and in healthy nonsmoking volunteers. The study also aimed to determine any relationship between eNO levels in COPD and: 1) conventional measures of lung function; and 2) inhaled corticosteroid (ICS) use.In former smokers with COPD, nonsmokers with asthma and volunteers, eNO levels, spirometry, lung volumes, carbon monoxide diffusion capacity of the lung (DL,CO) and resting oxygen saturation (Sa,O 2 ) were measured.Median eNO was significantly higher among patients with COPD than among healthy volunteers (p~0.003) but lower than among patients with asthma (pv0.01). There was no significant difference in eNO levels between COPD patients using ICS and those not using ICS. By contrast, eNO was lower among asthma patients who used ICS (median 32 parts per billion (ppb); 25 -75% range 16 -54) than among asthma patients who did not (51 ppb; 32 -87) (p~0.034). Among patients with COPD, eNO was inversely correlated with forced expiratory volume in one second, DL,CO and Sa,O 2 , and was positively correlated with the residual lung volume/total lung capacity ratio. Among patients with asthma, no significant correlations were found.Exhaled nitric oxide is increased in patients with chronic obstructive pulmonary disease, an increase that is influenced by structural abnormalities of tobacco-induced lung damage. Eur Respir J 2001; 17: 934-938.

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“…Several groups of investigators have attempted to describe eNO values in COPD previously with conflicting results [12]. There are many possible explanations for such inconsistant results.…”

Section: Discussionmentioning

confidence: 99%

Exhaled nitric oxide in chronic obstructive pulmonary disease: relationship to pulmonary function

Ansarin¹,

Chatkin²,

Ferreira³

et al. 2001

Eur Respir J

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“…137 The use of patient groups with differing disease severity and the inclusion or exclusion of current smokers in the analyses may explain the differences in exhaled NO levels in patients with COPD. 138 Specifically, disease severity may be an important factor since increased exhaled NO levels have been reported in patients admitted to hospital with an exacerbation of COPD, whereas NO levels were found not to differ from control values months after discharge of these patients. 139 Several studies have been performed to assess the relationship between levels of exhaled NO and lung function parameters and other markers of airway inflammation.…”

Section: Exhaled No As a Non-invasive Biomarkermentioning

confidence: 96%

Multiple roles of nitric oxide in the airways

Ricciardolo¹

2003

Thorax

Self Cite

319

292

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“…Exhaled NO levels in COPD are not elevated as in asthma and may be reduced by cigarette smoking (8,9). Furthermore, exhaled NO levels are not reduced by steroid treatment (10).…”

mentioning

confidence: 91%

Nitric Oxide Synthase Isoenzyme Expression and Activity in Peripheral Lung Tissue of Patients with Chronic Obstructive Pulmonary Disease

Brindicci

Kharitonov²,

Ito³

et al. 2010

Am J Respir Crit Care Med

101

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Rationale: Nitric oxide (NO) is increased in the lung periphery of patients with chronic obstructive pulmonary disease (COPD). However, expression of the NO synthase(s) responsible for elevated NO has not been identified in the peripheral lung tissue of patients with COPD of varying severity. Objectives:Methods: Protein and mRNA expression of nitric oxide synthase type I (neuronal NOS [nNOS]), type II (inducible NOS [iNOS]), and type III (endothelial NOS [eNOS]) were quantified by Western blotting and reverse transcription-polymerase chain reaction, respectively, in specimens of surgically resected lung tissue from nonsmoker control subjects, patients with COPD of varying severity, and smokers without COPD, and in a lung epithelial cell line (A549). The effects of nitrative/oxidative stress on NOS expression and activity were also evaluated in vitro in A549 cells. nNOS nitration was quantified by immunoprecipitation and dimerization of nNOS was detected by low-temperature SDS-PAGE/Western blot in the presence of the peroxynitrite generator, 3-morpholinosydnonimine-N-ethylcarbamide (SIN1), in vitro and in vivo. Measurements and Main Results:Lung tissue from patients with severe and very severe COPD had graded increases in nNOS (mRNA and protein) compared with nonsmokers and normal smokers. Hydrogen peroxide (H 2 O 2 ) and SIN1 as well as the cytokine mixture (IFN-γ, IL-1β, and tumor necrosis factor-α) increased mRNA expression and activity of nNOS in A549 cells in a concentration-dependent manner compared with nontreated cells. Tyrosine nitration resulted in an increase in nNOS activity in vitro, but did not affect its dimerization. Conclusions:Patients with COPD have a significant increase in nNOS expression and activity that reflects the severity of the disease and may be secondary to oxidative stress.

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Exhaled nitric oxide in COPD: glancing through a smoke screen

Cited by 31 publications

References 72 publications

Exhaled nitric oxide in chronic obstructive pulmonary disease: relationship to pulmonary function

Exhaled nitric oxide in chronic obstructive pulmonary disease: relationship to pulmonary function

Multiple roles of nitric oxide in the airways

Nitric Oxide Synthase Isoenzyme Expression and Activity in Peripheral Lung Tissue of Patients with Chronic Obstructive Pulmonary Disease

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