Exiguobacterium enclense sp. nov., isolated from sediment

Dastager, Syed G.; Mawlankar, Rahul; Sonalkar, Vidya V.; Thorat, Meghana N.; Mual, Poonam; Verma, Ashish; Krishnamurthi, Srinivasan; Tang, Shan-Kun; Li, Wenjun

doi:10.1099/ijs.0.000149

Cited by 27 publications

(12 citation statements)

References 34 publications

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“…The effect of arsenic in stimulating bacterial growth has been previously reported [ 99 ] and has been related to a positive effect on bacteria metabolism, resulting in a shorter generation time and higher cell yield. On the other hand, the observed effect may be related to the addition of sodium (since arsenic was added as Na 2 AsO 4 ), which may promote bacterial growth [ 100 ].…”

Section: Resultsmentioning

confidence: 99%

Functional annotation of hypothetical proteins from the Exiguobacterium antarcticum strain B7 reveals proteins involved in adaptation to extreme environments, including high arsenic resistance

et al. 2018

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Exiguobacterium antarcticum strain B7 is a psychrophilic Gram-positive bacterium that possesses enzymes that can be used for several biotechnological applications. However, many proteins from its genome are considered hypothetical proteins (HPs). These functionally unknown proteins may indicate important functions regarding the biological role of this bacterium, and the use of bioinformatics tools can assist in the biological understanding of this organism through functional annotation analysis. Thus, our study aimed to assign functions to proteins previously described as HPs, present in the genome of E. antarcticum B7. We used an extensive in silico workflow combining several bioinformatics tools for function annotation, sub-cellular localization and physicochemical characterization, three-dimensional structure determination, and protein-protein interactions. This genome contains 2772 genes, of which 765 CDS were annotated as HPs. The amino acid sequences of all HPs were submitted to our workflow and we successfully attributed function to 132 HPs. We identified 11 proteins that play important roles in the mechanisms of adaptation to adverse environments, such as flagellar biosynthesis, biofilm formation, carotenoids biosynthesis, and others. In addition, three predicted HPs are possibly related to arsenic tolerance. Through an in vitro assay, we verified that E. antarcticum B7 can grow at high concentrations of this metal. The approach used was important to precisely assign function to proteins from diverse classes and to infer relationships with proteins with functions already described in the literature. This approach aims to produce a better understanding of the mechanism by which this bacterium adapts to extreme environments and to the finding of targets with biotechnological interest.

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Section: Resultsmentioning

confidence: 99%

Functional annotation of hypothetical proteins from the Exiguobacterium antarcticum strain B7 reveals proteins involved in adaptation to extreme environments, including high arsenic resistance

et al. 2018

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“…On the genus level, the majority of isolates belonged to the well-studied Pseudoalteromonas genus, 27 which is known for its diverse secondary metabolite production and morphogenic activity. 5,28 In addition, several members of the geographically widely distributed Cobetia , 29 Exiguobacterium 30 and Psychrobacter 31 genera were obtained.…”

Section: Resultsmentioning

confidence: 99%

Natural products and morphogenic activity of γ-Proteobacteria associated with the marine hydroid polyp Hydractinia echinata

Guo

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Sperfeld

et al. 2017

Bioorganic & Medicinal Chemistry

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Illumina 16S rRNA gene sequencing was used to profile the associated bacterial community of the marine hydroid Hydractinia echinata, a long-standing model system in developmental biology. 56 associated bacteria were isolated and evaluated for their antimicrobial activity. Three strains were selected for further in-depth chemical analysis leading to the identification of 17 natural products. Several γ-Proteobacteria were found to induce settlement of the motile larvae, but only six isolates induced the metamorphosis to the primary polyp stage within 24 h. Our study paves the way to better understand how bacterial partners contribute to protection, homeostasis and propagation of the hydroid polyp.

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“…Genomic analysis shows that some type of genetic redundancy related to osmotolerance functions may increase the ability of some strains ( E. marinum DSM 16307, Exiguobacterium enclense NIO-1109, E. undae 190-11) to tolerate higher concentrations of salts in their environment. This stems from the identification of a positive correlation between the genomes that have the largest number of copies of some genes ( opuCA and gltR ) and their reported resistance ( Figure 5 and Supplementary Table S1 ; Rodrigues et al, 2006 ; Dastager et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization and Salt Response in Recurrent Halotolerant Exiguobacterium sp. SH31 Isolated From Sediments of Salar de Huasco, Chilean Altiplano

et al. 2018

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Poly-extremophiles microorganisms have the capacity to inhabit hostile environments and can survive several adverse conditions that include as variations in temperature, pH, and salinity, high levels UV light and atmospheric pressure, and even the presence of toxic compounds and the formation of reactive oxygen species (ROS). A halotolerant Exiguobacterium strain was isolated from Salar de Huasco (Chilean Altiplano), a well-known shallow lake area with variable salinity levels, little human intervention, and extreme environmental conditions, which makes it ideal for the study of resistant mechanisms and the evolution of adaptations. This bacterial genus has not been extensively studied, although its cosmopolitan location indicates that it has high levels of plasticity and adaptive capacity. However, to date, there are no studies regarding the tolerance and resistance to salinity and osmotic pressure. We set out to characterize the Exiguobacterium sp. SH31 strain and describe its phenotypical and genotypical response to osmotic stress. In this context, as a first step to characterize the response to the SH31 strain to salinity and to establish the bases for a molecular study, we proposed to compare its response under three salt conditions (0, 25, and 50 g/l NaCl). Using different physiology, genomic, and transcriptomic approaches, we determined that the bacterium is able to grow properly in a NaCl concentration of up to 50 g/l; however, the best growth rate was observed at 25 g/l. Although the presence of flagella is not affected by salinity, motility was diminished at 25 g/l NaCl and abolished at 50 g/l. Biofilm formation was induced proportionally with increases in salinity, which was expected. These phenotypic results correlated with the expression of related genes: fliG and fliS Motility); opuBA and putP (transport); glnA, proC, gltA, and gbsA (compatible solutes); ywqC, bdlA, luxS y pgaC (biofilm and stress response); and therefore, we conclude that this strain effectively modifies gene expression and physiology in a differential manner when faced with different concentrations of NaCl and these modifications aid survival.

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Exiguobacterium enclense sp. nov., isolated from sediment

Abstract: A Gram-stain-positive bacterium, designated strain NIO-1109 T , was isolated from a marine sediment sample from Chorao Island, Goa, India.

Cited by 27 publications

References 34 publications

Functional annotation of hypothetical proteins from the Exiguobacterium antarcticum strain B7 reveals proteins involved in adaptation to extreme environments, including high arsenic resistance

Functional annotation of hypothetical proteins from the Exiguobacterium antarcticum strain B7 reveals proteins involved in adaptation to extreme environments, including high arsenic resistance

Natural products and morphogenic activity of γ-Proteobacteria associated with the marine hydroid polyp Hydractinia echinata

Characterization and Salt Response in Recurrent Halotolerant Exiguobacterium sp. SH31 Isolated From Sediments of Salar de Huasco, Chilean Altiplano

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