Existence of a typical threshold in the response of human mesenchymal stem cells to a peak and valley topography

Bigerelle, Maxence; Giljean, Sylvain; Anselme, Karine

doi:10.1016/j.actbio.2011.05.013

Cited by 36 publications

(25 citation statements)

References 45 publications

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“…To explain this absence of influence of roughness on cell behavior of isotropic‐polished surfaces, a much wider roughness range produced by electroerosion ( R a = 1.2 and 21 μm) was tested (Bigerelle et al ., ). It was found that cell behavior, also defined as cell number and surface‐covering rate, depends on the roughness.…”

Section: Discussionmentioning

confidence: 97%

“…Metallic materials like stainless steel 316L, titanium, titanium alloys, and chromium/cobalt alloys are frequently used in the biomedical field for the manufacture of prostheses (Geetha et al , ). The influence of metallic surface topography on cell behavior has been widely studied (Martin et al ., ; Lincks et al ., ; Soboyejo et al ., ; Kim et al ., ; Chen et al ., ; Le Guehennec et al ., ; Giljean et al ., ; Bigerelle et al ., ) using various processes such as plasma spraying, sandblasting, acid etching, anodization, etc. To obtain isotropic topographies over a wide range of dimensions from submicron to several microns, researchers generally compare surfaces obtained by various processes (Martin et al ., ; Lincks et al ., ; Soboyejo et al ., ; Kim et al ., ; Chen et al ., ; Le Guehennec et al ., ; Giljean et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Roughness statistical influence on cell adhesion using profilometry and multiscale analysis

2012

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In this study, two series of 11 samples of TiAl6V4 titanium alloy and 316L stainless steel have been polished in an isotropic manner at different levels in order to quantify the influence of biomaterial roughness on cell behavior. Topography was measured by a tactile profilometer and a multiscale analysis has been carried out. Human osteoblasts have been cultured on those samples. It appears that roughness has no reproducible effect on the cell behavior except an influence on cell orientation on the wider grooves. As a conclusion, biomaterial surface damage, in the roughness range between Ra = 0.01 and 0.1 μm, has no influence on cell-adhesion mechanisms when roughness is isotropic and groove width is inferior to a critical value.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Roughness statistical influence on cell adhesion using profilometry and multiscale analysis

2012

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“…2013), inducing specific cell responses (Bigerelle et al. 2011). Only recently, the use of electro-erosion (Bigerelle et al.…”

Section: Introductionmentioning

confidence: 99%

Stem cell mechanical behaviour modelling: substrate’s curvature influence during adhesion

Vassaux

Milan

2017

Biomech Model Mechanobiol

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Recent experiments hint that adherent cells are sensitive to their substrate curvature. It is already well known that cells behaviour can be regulated by the mechanical properties of their environment. However, no mechanisms have been established regarding the influence of cell-scale curvature of the substrate. Using a numerical cell model, based on tensegrity structures theory and the non-smooth contact dynamics method, we propose to investigate the mechanical state of adherent cells on concave and convex hemispheres. Our mechanical cell model features a geometrical description of intracellular components, including the cell membrane, the focal adhesions, the cytoskeleton filament networks, the stress fibres, the microtubules, the nucleus membrane and the nucleoskeleton. The cell model has enabled us to analyse the evolution of the mechanical behaviour of intracellular components with varying curvature radii and with the removal of part of these components. We have observed the influence of the convexity of the substrate on the cell shape, the cytoskeletal force networks as well as on the nucleus strains. The more convex the substrate, the more tensed the stress fibres and the cell membrane, the more compressed the cytosol and the microtubules, leading to a stiffer cell. Furthermore, the more concave the substrate, the more stable and rounder the nucleus. These findings achieved using a verified virtual testing methodology, in particular regarding the nucleus stability, might be of significant importance with respect to the division and differentiation of mesenchymal stem cells. These results can also bring some hindsights on cell migration on curved substrates.

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“…Changing the mean diameter of the CP granules modified the macroporosity of the monolayer, known to be an important factor for cell attachment and cell viability. [48][49][50][51] In particular, for HA/TCP particles, Mankani et al investigated in vivo bone formation for several size ranges. 52 Bone marrow stromal cells and HA/TCP particles were transplanted into mice for at least 4 weeks, and bone formation was estimated by semiquantitative observations and histomorphometric methods.…”

Section: Discussionmentioning

confidence: 99%

Towards the Development and Characterization of an Easy Handling Sheet-Like Biohybrid Bone Substitute

Baudequin

Bédoui

Dufresne

et al. 2015

Tissue Engineering Part A

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We designed a sheet-like bone substitute capable of adapting to different geometries and becoming a standard tissue-engineered process for bone surgery. Preosteoblastic cells were seeded on to a monolayer of calcium phosphate granules and cultured in a flat parallelepipedic cell culture chamber for 1 month. From the various diameters of the granules examined, the 80-200 μm group exhibited the most homogeneous performances regarding both biological (cell morphology, viability, differentiation, and simple metabolic activity) and mechanical (cohesion and stress-strain behavior) properties. This sheet was easy to handle after extraction from the culture chamber and showed versatile geometry and flexibility, making it easy to use for surgeons, especially for small defects of the maxillofacial area.

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Existence of a typical threshold in the response of human mesenchymal stem cells to a peak and valley topography

Cited by 36 publications

References 45 publications

Roughness statistical influence on cell adhesion using profilometry and multiscale analysis

Roughness statistical influence on cell adhesion using profilometry and multiscale analysis

Stem cell mechanical behaviour modelling: substrate’s curvature influence during adhesion

Towards the Development and Characterization of an Easy Handling Sheet-Like Biohybrid Bone Substitute

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