Exogenous abscisic acid enhances physiological, metabolic, and transcriptional cold acclimation responses in greenhouse-grown grapevines

Wang, Hongrui; Blakeslee, Joshua J.; Jones, Michelle L.; Chapin, Laura J.; Dami, Imed

doi:10.1016/j.plantsci.2020.110437

Cited by 31 publications

(16 citation statements)

References 70 publications

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“…3b), similarly to action realized with guard cellautonomously synthesized ABA (Bauer et al 2013), thus immediately restricting water loss. The rapid decrease of leaf stomatal conductance after the ABA application has been also documented by Wang et al (2020). In contrast, Put application was significantly less effective in rapid stomatal closure.…”

Section: Discussionmentioning

confidence: 68%

Effect of exogenously-applied abscisic acid, putrescine and hydrogen peroxide on drought tolerance of barley

Skowron

Trojak

2020

Biologia

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The objective of this study was to identify the effect of abscisic acid (ABA), putrescine (Put) and hydrogen peroxide (H2O2) foliar pre-treatment on drought tolerance of barley. Despite water limitation, ABA-sprayed plants preserved increased water content, photosynthetic efficiency of PSII (ΦPSII) and CO2 assimilation rate (Pn) compared to untreated stressed plants. The ABA-treated plants presented also the lowest rate of lipid peroxidation (MDA), lowered the rate of PSII primary acceptor reduction (1 – qP) and increased the yield of regulated energy dissipation (NPQ) with higher accumulation of PGRL1 (PROTON GRADIENT REGULATION LIKE1) protein. These plants preserved a similar level of photochemical efficiency and the rate of electron transport of PSII (ETRII) to the well-watered samples. The significantly less pronounced response was observed in Put-sprayed samples under drought. Additionally, the combined effects of drought and H2O2 application increased the 1 – qP and quantum yield of non-regulated energy dissipation in PSII (ΦNO) and reduced the accumulation of Rubisco activase (RCA). In conclusion, ABA foliar application allowed to balance water retention and preserve antioxidant capacity resulting in efficient photosynthesis and the restricted risk of oxidative damage under drought. Neither hydrogen peroxide nor putrescine has been able to ameliorate drought stress as effectively as ABA.

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Section: Discussionmentioning

confidence: 68%

Effect of exogenously-applied abscisic acid, putrescine and hydrogen peroxide on drought tolerance of barley

Skowron

Trojak

2020

Biologia

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“…The V. riparia data appear to be consistent with those seen in apple and other temperate/boreal species, where homologues of GALACTINOL SYNTHASE showed strong circannual rhythms, that were paralleled by the concentration of galactinol and raffinose ( Cox and Stushnoff, 2001 ; Derory et al , 2006 ; Pagter et al , 2008 ; Ibánez et al , 2013 ; Falavigna et al , 2018 ). Wang et al (2020) reported an increase in concentration of galactinol and stachyose in the explants following application of exogenous ABA. In the absence of exogenous ABA, however, there was little temporal change in sugar and oligosaccharide content ( Wang et al , 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“… Wang et al (2020) reported an increase in concentration of galactinol and stachyose in the explants following application of exogenous ABA. In the absence of exogenous ABA, however, there was little temporal change in sugar and oligosaccharide content ( Wang et al , 2020 ).…”

Section: Discussionmentioning

confidence: 99%

The bud dormancy disconnect: latent buds of grapevine are dormant during summer despite a high metabolic rate

Velappan

Chabikwa

Considine

et al. 2022

Journal of Experimental Botany

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Grapevine (Vitis vinifera L.) displays wide plasticity to climate, however the physiology of dormancy along a seasonal continuum is poorly understood. Here we investigated the apparent disconnect between dormancy and the underlying respiratory physiology and transcriptome of grapevine buds, from bud set in summer to bud burst in spring. The establishment of dormancy in summer was pronounced and reproducible, however this was coupled with little or no change in physiology, indicated by respiration, hydration and tissue oxygen tension. The release of dormancy was biphasic; the depth of dormancy declined substantially by mid-autumn, while the subsequent decline towards spring was moderate. Observed changes in physiology failed to explain the first phase of dormancy decline, in particular. Transcriptome data contrasting development from summer through to spring also indicated that dormancy was poorly reflected by metabolic quiescence during summer and autumn. Gene ontology and enrichment data revealed the prevailing influence of abscisic acid (ABA)-related gene expression during the transition from summer to autumn, and promoter motif analysis suggested photoperiod may play an important role in regulating ABA functions during the establishment of dormancy. Transcriptomic data from later transitions reinforced the importance of oxidation and hypoxia as physiological cues to regulate the maintenance of quiescence and resumption of growth. Collectively these data reveal a novel disconnect between growth and metabolic quiescence in grapevine following bud set, which requires further experimentation to explain the phenology and dormancy relationships.

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“…Notably, the gene expression up-regulation of six AcRFS genes ( AcRFS1/4/6/7/10 ) was induced transcriptionally in ABA treatment ( Figure 4 B), suggesting that activation of AcRFS gene might be positively correlated with ABA levels. Exogenous ABA-treated improved levels of VvRafs1 gene expression in grapevine buds [ 49 ]. Unsurprisingly, abiotic stresses induced GolS and RFS genes in plants [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Expression Profiling Analysis of Kiwifruit GolS and RFS Genes and Identification of AcRFS4 Function in Raffinose Accumulation

Yang

Ling

Liu

et al. 2022

IJMS

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The raffinose synthetase (RFS) and galactinol synthase (GolS) are two critical enzymes for raffinose biosynthesis, which play an important role in modulating plant growth and in response to a variety of biotic or abiotic stresses. Here, we comprehensively analyzed the RFS and GolS gene families and their involvement in abiotic and biotic stresses responses at the genome-wide scale in kiwifruit. A total of 22 GolS and 24 RFS genes were identified in Actinidia chinensis and Actinidia eriantha genomes. Phylogenetic analysis showed that the GolS and RFS genes were clustered into four and six groups, respectively. Transcriptomic analysis revealed that abiotic stresses strongly induced some crucial genes members including AcGolS1/2/4/8 and AcRFS2/4/8/11 and their expression levels were further confirmed by qRT-PCR. The GUS staining of AcRFS4Pro::GUS transgenic plants revealed that the transcriptionlevel of AcRFS4 was significantly increased by salt stress. Overexpression of AcRFS4 in Arabidopsis demonstrated that this gene enhanced the raffinose accumulation and the tolerance to salt stress. The co-expression networks analysis of hub transcription factors targeting key AcRFS4 genes indicated that there was a strong correlation between AcNAC30 and AcRFS4 expression under salt stress. Furthermore, the yeast one-hybrid assays showed that AcNAC30 could bind the AcRFS4 promoter directly. These results may provide insights into the evolutionary and functional mechanisms of GolS and RFS genes in kiwifruit.

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Exogenous abscisic acid enhances physiological, metabolic, and transcriptional cold acclimation responses in greenhouse-grown grapevines

Cited by 31 publications

References 70 publications

Effect of exogenously-applied abscisic acid, putrescine and hydrogen peroxide on drought tolerance of barley

Effect of exogenously-applied abscisic acid, putrescine and hydrogen peroxide on drought tolerance of barley

The bud dormancy disconnect: latent buds of grapevine are dormant during summer despite a high metabolic rate

Genome-Wide Expression Profiling Analysis of Kiwifruit GolS and RFS Genes and Identification of AcRFS4 Function in Raffinose Accumulation

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