Exogenous adenosine 5′-phosphoramidate behaves as a signal molecule in plants; it augments metabolism of phenylpropanoids and salicylic acid in Arabidopsis thaliana seedlings

Pietrowska‐Borek, Małgorzata; Nuc, Katarzyna; Guranowski, Andrzej

doi:10.1016/j.plaphy.2015.05.013

Cited by 8 publications

(7 citation statements)

References 59 publications

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“…Finally, NH 2 -pA should be considered as a novel signal molecule. Recently, this idea has gained support from experiments performed in our laboratory on Arabidopsis seedlings [26]. Micromolar concentration of exogenous NH 2 -pA added to the growth medium induced in these seedlings activity of several genes that encode enzymes catalysing key reactions of phenylpropanoid pathways and caused accumulation of lignins, anthocyanins and salicylic acid, the compounds that appear in the plant cells in response to stress.…”

Section: Results and Discussisonmentioning

confidence: 99%

Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins

Wojdyła-Mamoń

Guranowski

2015

Bioscience Reports

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SynopsisFhits (fragile histidine triad proteins) occur in eukaryotes but their function is largely unknown, although human Fhit is believed to act as a tumour suppressor. Fhits also exhibit dinucleoside triphosphatase, adenylylsulfatase and nucleoside phosphoramidase activities that in each case yield nucleoside 5 -monophosphate as a product. Due to the dinucleoside triphosphatase activity, Fhits may also be involved in mRNA decapping. In the present study, we demonstrate Fhit-catalysed ammonolysis of adenosine 5 -phosphosulfate, which results in the formation of adenosine 5 -phosphoramidate. This reaction has previously been associated with adenylylsulfate-ammonia adenylyltransferase (EC 2.7.7.51). Our finding shows that the capacity to catalyse ammonolysis is another inherent property of Fhits. Basic kinetic parameters and substrate specificity of this reaction catalysed by human Fhit are presented.

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Section: Results and Discussisonmentioning

confidence: 99%

Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins

Wojdyła-Mamoń

Guranowski

2015

Bioscience Reports

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“…Another important effect caused by NH 2 -pA was an increase in the ICS gene expression and a significant increase (2-fold) in the level of free salicylic acid. In view of the fact that salicylic acid is one of the signaling molecules involved in the response of plants to biotic stress, the induction of the synthesis of salicylic acid by NH 2 -pA may suggest the interaction of both these compounds in plant responses to stresses [187].…”

Section: Discussionmentioning

confidence: 99%

“…So far there are only a few papers describing the role of uncommon nucleotides in plant cells. It was shown that the phenylpropanoid pathway may be affected by exogenous uncommon nucleotides [185][186][187][188]. The phenylpropanoid pathway is a source of secondary metabolites which are very important in plant responses to biotic and abiotic stresses [122].…”

Section: Function Of Uncommon Nucleotidesmentioning

confidence: 99%

New Insight into Plant Signaling: Extracellular ATP and Uncommon Nucleotides

Pietrowska‐Borek

Dobrogojski

Sobieszczuk‐Nowicka

et al. 2020

Cells

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New players in plant signaling are described in detail in this review: extracellular ATP (eATP) and uncommon nucleotides such as dinucleoside polyphosphates (NpnN’s), adenosine 5′-phosphoramidate (NH2-pA), and extracellular NAD+ and NADP+ (eNAD(P)+). Recent molecular, physiological, and biochemical evidence implicating concurrently the signaling role of eATP, NpnN’s, and NH2-pA in plant biology and the mechanistic events in which they are involved are discussed. Numerous studies have shown that they are often universal signaling messengers, which trigger a signaling cascade in similar reactions and processes among different kingdoms. We also present here, not described elsewhere, a working model of the NpnN’ and NH2-pA signaling network in a plant cell where these nucleotides trigger induction of the phenylpropanoid and the isochorismic acid pathways yielding metabolites protecting the plant against various types of stresses. Through these signals, the plant responds to environmental stimuli by intensifying the production of various compounds, such as anthocyanins, lignin, stilbenes, and salicylic acid. Still, more research needs to be performed to identify signaling networks that involve uncommon nucleotides, followed by omic experiments to define network elements and processes that are controlled by these signals.

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“…Phenylpropanoid biosynthetic pathway was also induced by pathogen infection [71,72]. AT2G30490 encodes a cinnamate-4-hydroxylase (C4H), which catalyzes key reactions of phenylpropanoid pathways and modulates salicylic acid accumulation [73]. Recently, the biosynthetic pathways for phenylalanine and phenylalanine-derived compounds were activated for the induction of ETI [74], which gives inspirations for analyzing the role of phenylpropanoids during the interaction between plant-parasitic nematodes and hosts.…”

Section: Discussionmentioning

confidence: 99%

MiMIF-2 Effector of Meloidogyne incognita Exhibited Enzyme Activities and Potential Roles in Plant Salicylic Acid Synthesis

Zhao

Mao

Sun

et al. 2020

IJMS

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Plant-parasitic nematodes secrete a series of effectors to promote parasitism by modulating host immunity, but the detailed molecular mechanism is ambiguous. Animal parasites secrete macrophage migration inhibitory factor (MIF)-like proteins for evasion of host immune systems, in which their biochemical activities play essential roles. Previous research demonstrated that MiMIF-2 effector was secreted by Meloidogyne incognita and modulated host immunity by interacting with annexins. In this study, we show that MiMIF-2 had tautomerase activity and protected nematodes against H2O2 damage. MiMIF-2 expression not only decreased the amount of H2O2 generation during nematode infection in Arabidopsis, but also suppressed Bax-induced cell death by inhibiting reactive oxygen species burst in Nicotiana benthamiana. Further, RNA-seq transcriptome analysis and RT-qPCR showed that the expression of some heat-shock proteins was down regulated in MiMIF-2 transgenic Arabidopsis. After treatment with flg22, RNA-seq transcriptome analysis indicated that the differentially expressed genes in MiMIF-2 expressing Arabidopsis were pointed to plant hormone signal transduction, compound metabolism and plant defense. RT-qPCR and metabolomic results confirmed that salicylic acid (SA) related marker genes and SA content were significantly decreased. Our results provide a comprehensive understanding of how MiMIF-2 modulates plant immunity and broaden knowledge of the intricate relationship between M. incognita and host plants.

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Exogenous adenosine 5′-phosphoramidate behaves as a signal molecule in plants; it augments metabolism of phenylpropanoids and salicylic acid in Arabidopsis thaliana seedlings

Cited by 8 publications

References 59 publications

Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins

Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins

New Insight into Plant Signaling: Extracellular ATP and Uncommon Nucleotides

MiMIF-2 Effector of Meloidogyne incognita Exhibited Enzyme Activities and Potential Roles in Plant Salicylic Acid Synthesis

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