Exogenous administration of appropriate dose of IL-17A helps to improve multiple organ dysfunction and improve survival in septic mice

Abstract

Purpose To explore the effect of exogenous interleukin-17A on multi-organ function and prognosis in septic mice. Methods Mice model of sepsis was established by cecal ligation and puncture (CLP) method, and the mice were randomly divided into 8 groups according to different intervention measures: Sham + PBS, CLP + PBS, CLP + 0.25µg IL-17A, CLP + 0.5µg IL-17A, CLP + 1µg IL-17A, CLP + 2µg IL-17A, CLP + 4µg IL-17A and CLP + Anti-IL-17A. Survival rates of each group were monitored and recorded at 12-hour intervals. The blood of each group of mice was collected at each observation node under aseptic condition, and the expression changes of blood routine, related inflammatory cytokines, liver and kidney function indexes. Pathological injuries of lung, liver and kidney of mice in each group were detected by H&E staining, and the evaluation of bacterial translocation in vitro was performed by inoculation medium. Results Except for the sham-operated group, the 7-day survival rate of the mice in the CLP + 1µg IL-17A group was the highest (75%) and exogenous administration of appropriate dose of IL-17A was beneficial to reduce alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatinine (Cre) in septic mice. The pathological damage of lung, liver and kidney tissue also can be alleviated, and the colony count of peripheral blood and spleen tissue of mice were also significantly decreased. Conclusion Exogenous administration of appropriate dose of IL-17A can improve the bacterial clearance ability of septic mice, and improve the multiple organ dysfunction and 7-day survival rate of the septic mice.