Exogenous adrenocorticotropic hormone affects genome‐wide DNA methylation and transcriptome of corpus luteum in sows

Zhao, Fen; Wu, Wangjun; Wei, Quanwei; Shen, Ming; Li, Bojiang; Jiang, Yi; Liu, Kaiqing; Liu, Honglin

doi:10.1096/fj.201801081rrr

Cited by 7 publications

(9 citation statements)

References 62 publications

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“…A heat map was generated by a cluster analysis of DMRs between the WT and KO groups ( Figure 5 A). The length distribution of DMRs ranged from dozens to hundreds of nucleotides and complied with the Gaussian distribution ( Figure 5 B), which is consistent with previous studies [ 60 , 61 ]. Violin boxplot was used to plot mean methylation levels, which showed that DMR methylation levels were mostly at medium level and not at low or high levels, and the mean methylation level was higher after miR-143 knock-out ( Figure 5 C).…”

Section: Resultssupporting

confidence: 91%

Exploration of the Effect on Genome-Wide DNA Methylation by miR-143 Knock-Out in Mice Liver

Chen¹,

Luo²,

Liu³

et al. 2021

IJMS

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MiR-143 play an important role in hepatocellular carcinoma and liver fibrosis via inhibiting hepatoma cell proliferation. DNA methyltransferase 3 alpha (DNMT3a), as a target of miR-143, regulates the development of primary organic solid tumors through DNA methylation mechanisms. However, the effect of miR-143 on DNA methylation profiles in liver is unclear. In this study, we used Whole-Genome Bisulfite Sequencing (WGBS) to detect the differentially methylated regions (DMRs), and investigated DMR-related genes and their enriched pathways by miR-143. We found that methylated cytosines increased 0.19% in the miR-143 knock-out (KO) liver fed with high-fat diet (HFD), compared with the wild type (WT). Furthermore, compared with the WT group, the CG methylation patterns of the KO group showed lower CG methylation levels in CG islands (CGIs), promoters and hypermethylation in CGI shores, 5′UTRs, exons, introns, 3′UTRs, and repeat regions. A total of 984 DMRs were identified between the WT and KO groups consisting of 559 hypermethylation and 425 hypomethylation DMRs. Furthermore, DMR-related genes were enriched in metabolism pathways such as carbon metabolism (serine hydroxymethyltransferase 2 (Shmt2), acyl-Coenzyme A dehydrogenase medium chain (Acadm)), arginine and proline metabolism (spermine synthase (Sms), proline dehydrogenase (Prodh2)) and purine metabolism (phosphoribosyl pyrophosphate synthetase 2 (Prps2)). In summary, we are the first to report the change in whole-genome methylation levels by miR-143-null through WGBS in mice liver, and provide an experimental basis for clinical diagnosis and treatment in liver diseases, indicating that miR-143 may be a potential therapeutic target and biomarker for liver damage-associated diseases and hepatocellular carcinoma.

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Section: Resultssupporting

confidence: 91%

Exploration of the Effect on Genome-Wide DNA Methylation by miR-143 Knock-Out in Mice Liver

Chen¹,

Luo²,

Liu³

et al. 2021

IJMS

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“…In accordance with the results of previous studies, the cortisol concentrations in the ACTH group (1 IU/kg BW) were three times higher than those in the control group on the first day of the experiment. Following the experiment, although the cortisol concentration in the ACTH group was reduced, it remained significantly higher than that in the control group [27], which could be attributed to the iterations of ACTH.…”

Section: Discussionmentioning

confidence: 76%

Impact of adrenocorticotropin hormone administration on the endocrinology, estrus onset, and ovarian function of weaned sows

Zhao

Wei

et al. 2022

Endocr J

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Chronic stress affects the reproductive health of mammals; however, the impact of adrenocorticotropin hormone (ACTH) level elevation during chronic stress on the reproduction of weaned sows remains unclear. In this study, nine weaned sows with the same parturition date were randomly divided into control group (n = 4) and ACTH group (n = 5). Each group received intravenous administration of ACTH three times daily for 7 days. Blood samples were collected every 3 h after injection. A radioimmunoassay was used to measure the concentrations of cortisol, luteinizing hormone (LH), folliclestimulating hormone (FSH), progesterone (P 4 ) and estradiol-17β (E 2 ) in the blood. Estrus was determined according to changes in the vulva and the boar contact test. The mRNA expressions of glucocorticoid receptor, FSH receptor, LH receptor (LHR) in the corpus luteum (CL) were detected by qRT-PCR. The results showed that ACTH administration substantially delayed the initiation of estrus and the pre-ovulatory LH peak. The sows of control group ovulated within 10 days and the ovulation rate was 100%, while it was 60% in the ACTH group. Two sows of ACTH group showed pseudo-estrus. The E 2 concentrations significantly decreased in the ACTH group at 36 h, 42 h and 66 h of the experimental period. The P 4 concentrations in the ACTH group significantly decreased at 132, 138, and 147 h of the experimental period. ACTH significantly reduced the LHR mRNA expression in CLs. In conclusion, long-term repeated ACTH administration affects the endocrinology, estrus onset, and ovarian function of weaned sows.

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“…DNA methylation assays for selected sequences of ADH1C , BGN , PSAT1 , and RSSF1 genes were designed earlier [ 26 , 35 ]. The region of interest for the DNA methylation assays of ADAMTS20 , DMBT1 , and WNT5A was chosen based on results from methylome sequencing of porcine corpora lutea [ 36 ] and embryos [ 37 ]. Briefly, genes of interest were selected based on our results from endometrial transcriptome profiling [ 4 , 25 ] and compared with genes annotated to DMRs identified in porcine CLs and/or embryos [ 36 , 37 ].…”

Section: Methodsmentioning

confidence: 99%

Novel role for conceptus signals in mRNA expression regulation by DNA methylation in porcine endometrium during early pregnancy

Kaczyński

Weijden

Goryszewska-Szczurek

et al. 2022

Biology of Reproduction

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During early pregnancy, porcine conceptuses (the embryos with associated membranes) secrete estradiol-17β (E2)—their major signal for maternal recognition of pregnancy—and prostaglandin E2 (PGE2). Both hormones induce prominent changes of the endometrial transcriptome in vivo. Studies on endometrial pathologies have shown that E2 affects gene expression by epigenetic mechanisms related to DNA methylation. Herein, we determined the effects of E2 and PGE2 alone, and a combined E2 + PGE2 treatment administered into the uterine lumen in vivo on the expression and activity of DNA-methyltransferases (DNMT) and on CpG methylation patterns of selected genes in porcine endometrium. To compare the effect of treatment with the physiological effect of pregnancy, endometria from day 12 pregnant/cyclic gilts were included. Both E2 and PGE2 significantly reduced the expression of DNMTs. Likewise, the expressions of DNMT1 and DNMT3A were decreased on day 12 of pregnancy compared to the estrous cycle. DNMT activity increased in endometrial samples following E2 treatment and in gilts on day 12 of pregnancy. Treatment with E2 alone and/or simultaneously with PGE2 altered endometrial DNA methylation of CpG sites of ADAMTS20, ADH1C, BGN, PSAT1 and WNT5A. Different CpG methylation patterns of ADAMTS20, BGN, DMBT1, RASSF1 and WNT5A were found in the endometrium on day 12 of pregnancy compared to day 12 of the estrous cycle. Significant correlations were detected between CpG methylation and gene expression for ADAMTS20, ADH1C, BGN, DMBT1, PSAT1 and WNT5A. Our results indicate that CpG methylation induced by embryonic signals may contribute to regulating endometrial gene expression during pregnancy establishment.

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Exogenous adrenocorticotropic hormone affects genome‐wide DNA methylation and transcriptome of corpus luteum in sows

Cited by 7 publications

References 62 publications

Exploration of the Effect on Genome-Wide DNA Methylation by miR-143 Knock-Out in Mice Liver

Exploration of the Effect on Genome-Wide DNA Methylation by miR-143 Knock-Out in Mice Liver

Impact of adrenocorticotropin hormone administration on the endocrinology, estrus onset, and ovarian function of weaned sows

Novel role for conceptus signals in mRNA expression regulation by DNA methylation in porcine endometrium during early pregnancy

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