Exogenous and endogenous force regulation of endothelial cell behavior

Califano, Joseph P.; Reinhart‐King, Cynthia A.

doi:10.1016/j.jbiomech.2009.09.012

Cited by 111 publications

(89 citation statements)

References 123 publications

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“…Capillary endothelium permeability and endothelial cell motility are modulated by mechanical forces that are conveyed by the ECM and focal adhesion formation (19)(20)(21)(22)(23)(24). To determine the mechanism by which LPP modulates endothelial cell motility and microvessel permeability, we used immunofluorescence microscopy to assess the colocalization of LPP and key proteins associated with focal adhesions, including paxillin, FAK, and vinculin.…”

Section: Silencing Of Mfap5 Downregulates Endothelial Lpp Expression mentioning

confidence: 99%

Cancer-associated fibroblasts regulate endothelial adhesion protein LPP to promote ovarian cancer chemoresistance

Leung

Yeung

Yip

et al. 2017

Journal of Clinical Investigation

114

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Section: Silencing Of Mfap5 Downregulates Endothelial Lpp Expression mentioning

confidence: 99%

Cancer-associated fibroblasts regulate endothelial adhesion protein LPP to promote ovarian cancer chemoresistance

Leung

Yeung

Yip

et al. 2017

Journal of Clinical Investigation

114

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“…Inspired by the work of Thoma (Thoma, 1893), who first proposed that blood circulation could regulate vessel morphogenesis, numerous studies have investigated the effects of shear stress and pressure on ECs (Califano and Reinhart-King, 2010;Chiu and Chien, 2011;Culver and Dickinson, 2010;Hahn and Schwartz, 2009;Li et al, 2005). Within the embryo, shear stress levels have been estimated to be ~5 dyn/cm 2 (Jones et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Dynamic responses of endothelial cells to changes in blood flow during vascular remodeling of the mouse yolk sac

2013

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SUMMARYDespite extensive work showing the importance of blood flow in angiogenesis and vessel remodeling, very little is known about how changes in vessel diameter are orchestrated at the cellular level in response to mechanical forces. To define the cellular changes necessary for remodeling, we performed live confocal imaging of cultured mouse embryos during vessel remodeling. Our data revealed that vessel diameter increase occurs via two distinct processes that are dependent on normal blood flow: vessel fusions and directed endothelial cell migrations. Vessel fusions resulted in a rapid change in vessel diameter and were restricted to regions that experience the highest flow near the vitelline artery and vein. Directed cell migrations induced by blood flow resulted in the recruitment of endothelial cells to larger vessels from smaller capillaries and were observed in larger artery segments as they expanded. The dynamic and specific endothelial cell behaviors captured in this study reveal how sensitive endothelial cells are to changes in blood flow and how such responses drive vascular remodeling.

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“…Vascular endothelial cells line the interior surface of blood vessels and are, hence, directly subjected to the physical forces exerted by the flow of blood and blood pressure (Ali and Schumacker, 2002;Califano and Reinhart-King, 2010). To withstand these forces it is conceivable that they have to be both mechanically robust and, to a certain degree, compliant (Malek and Izumo, 1996;Michiels, 2003).…”

Section: Introductionmentioning

confidence: 99%

Membrane potential depolarization decreases the stiffness of vascular endothelial cells

Callies

Fels

Liashkovich

et al. 2011

Journal of Cell Science

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The stiffness of vascular endothelial cells is crucial to mechanically withstand blood flow and, at the same time, to control deformation-dependent nitric oxide release. However, the regulation of mechanical stiffness is not yet understood. There is evidence that a possible regulator is the electrical plasma membrane potential difference. Using a novel technique that combines fluorescence-based membrane potential recordings with atomic force microscopy (AFM)-based stiffness measurements, the present study shows that membrane depolarization is associated with a decrease in the stiffness of endothelial cells. Three different depolarization protocols were applied, all of which led to a similar and significant decrease in cell stiffness, independently of changes in cell volume. Moreover, experiments using the actin-destabilizing agent cytochalasin D indicated that depolarization acts by affecting the cortical actin cytoskeleton. A model is proposed whereby a change of the electrical field across the plasma membrane is directly sensed by the submembranous actin network, regulating the actin polymerization:depolymerization ratio and thus cell stiffness. This depolarization-induced decrease in the stiffness of endothelial cells could play a role in flow-mediated nitric-oxide-dependent vasodilation.

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Exogenous and endogenous force regulation of endothelial cell behavior

Cited by 111 publications

References 123 publications

Cancer-associated fibroblasts regulate endothelial adhesion protein LPP to promote ovarian cancer chemoresistance

Cancer-associated fibroblasts regulate endothelial adhesion protein LPP to promote ovarian cancer chemoresistance

Dynamic responses of endothelial cells to changes in blood flow during vascular remodeling of the mouse yolk sac

Membrane potential depolarization decreases the stiffness of vascular endothelial cells

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