Exogenous antigens and the stimulation of MHC class I restricted cell-mediated cytotoxicity: possible strategies for fish vaccines

Dijkstra, Johannes M.; Fischer, Uwe; Sawamoto, Yoshihiro; Ototake, Mitsuru; Nakanishi, Teruyuki

doi:10.1006/fsim.2001.0351

Cited by 29 publications

(16 citation statements)

References 106 publications

(106 reference statements)

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“…Specific cell mediated cytotoxicity which has only recently been shown in fish is involved in the immune response against viral infection and cancer cells (reviewed by Nakanishi et al, 2002;Dijkstra et al, 2001). Sensitized sIgM − lymphocytes kill allogeneic cells or virus infected fish cells (Somamoto et al, 2000;Fischer et al, 1998;Stuge et al, 1997Stuge et al, , 1995.…”

Section: Specific Cell Mediated Cytotoxicitymentioning

confidence: 99%

Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss)—how can environmental influences be detected?

et al. 2002

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In fish, the first line of defense against infectious microorganisms is based on a broad range of nonspecific humoral and cellular immune mechanisms ('innate immunity') which without prior specific activation can act in forming a more static barrier (Fish Shellfish Immunol. 10 (2000) 243; Dev. Comp. Immunol. 25 (2001) 827). This natural resistance is normally effective enough to protect fish from infectious diseases until specific immune responses are being induced ( Fig. 1; Dev. Comp. Immunol. 25 (2001) 841). Healthy fish exhibit both nonspecific and specific immune responses depending directly on environmental temperature. Pollution of the natural aquatic environment with industrial or agricultural sewage is an important immunosuppressing factor resulting in higher susceptibility to infectious diseases. To date, the possible immunotoxicity of a substance is evaluated using quantification of humoral factors like lysozyme, complement, C-reactive protein or total immunoglobulins but less often using functional assays. Furthermore, most of the functional assays (phagocytosis, respiratory burst, proliferative response) are based on the measurement of the response of resting but not of specific activated immune cells. However, the physiological responses of the immune system to an infection are based on a complex, stepwise activation and proliferation, especially of the specific immune functions after first contact to the microorganisms. In this report we describe in vitro methods for the evaluation of cellular immune functions of different leukocyte populations after specific in vivo triggering of the immune system. Parameters to be evaluated are activation and proliferation of leukocyte populations, phagocytosis and respiratory burst, secretion of antigen-specific antibodies and specific cell-mediated cytotoxicity. Furthermore, challenge models with bacterial (Aeromonas salmonicida) and viral pathogens (Viral Haemorrhagic Septicemia Virus, VHSV) are presented.

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Section: Specific Cell Mediated Cytotoxicitymentioning

confidence: 99%

Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss)—how can environmental influences be detected?

et al. 2002

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“…However, the main findings were, (a) that the existing tools for choosing peptide sequences require knowledge on the structure of proteins which is often not available for fish viruses; (b) the tools based on mammalian models must be used with caution [36], but the main obstacle, the current lack of knowledge on antigen presentation processes in fish, should rapidly reduce [33,34] (c) the production process, the analyses of peptides before use and the choice of carrier protein as a function of the species are also important aspects of this research (d) that the theoretical immunogenicity, immunogenicity against a peptide, cross-reaction with the virus, activity in vitro and protection in vivo are often related but do not necessarily follow one from the other and the challenge is the last, crucial test. However, if a valid strategy is to be found for choosing peptides to be used as vaccines in fish, it will be necessary to continue to examine all of the intermediary steps.…”

Section: Discussionmentioning

confidence: 98%

Preliminary approach to find synthetic peptides from nodavirus capsid potentially protective against sea bass viral encephalopathy and retinopathy

Coeurdacier

Laporte

Pépin

2003

Fish & Shellfish Immunology

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“…Nevertheless, it is not unreasonable to conclude that fi sh can develop an (Chen et al, 2010), may provide further evidence to support this conclusion. In teleost fi sh, it was once considered that endogenous and exogenous antigens were presented by MHC I and MHC II molecules, respectively (see Dijkstra et al, 2001 for a discussion on this). But, exogenous antigens can be off ered by MHC class I molecules when they are packed into or connected to a structure that delivers them into the cytoplasm (Dijkstra et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…In teleost fi sh, it was once considered that endogenous and exogenous antigens were presented by MHC I and MHC II molecules, respectively (see Dijkstra et al, 2001 for a discussion on this). But, exogenous antigens can be off ered by MHC class I molecules when they are packed into or connected to a structure that delivers them into the cytoplasm (Dijkstra et al, 2001). A signifi cant increase in the expression of the MHC Iα gene was found also in Japanese fl ounder vaccinated with recombinant Eta2, an exogenous protein (Sun et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella

Luo

Liu

et al. 2016

Chin. J. Ocean. Limnol.

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Flavobacterium columnare causes columnaris disease in freshwater fi sh. In the present study, the antigenic regions of fi ve outer membrane proteins (OMPs), including zinc metalloprotease, prolyl oligopeptidase, thermolysin, collagenase and chondroitin AC lyase, were bioinformatically analyzed, fused together, and then expressed as a recombinant fusion protein in Escherichia coli . The expressed protein of 95.6 kDa, as estimated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was consistent with the molecular weight deduced from the amino acid sequence. The purifi ed recombinant protein was used to vaccinate the grass carp, Ctenopharyngodon idella . Following vaccination of the fi sh their IgM antibody levels were examined, as was the expression of IgM , IgD and IgZ immunoglobulin genes and other genes such as MHC Iα and MHC I I β , which are also involved in adaptive immunity. Interleukin genes ( IL ), including IL -1β , IL -8 and IL -10 , and type I and type II interferon ( IFN ) genes were also examined. At 3 and 4 weeks post-vaccination (wpv), signifi cant increases in IgM antibody levels were observed in the fi sh vaccinated with the recombinant fusion protein, and an increase in the expression levels of IgM , IgD and IgZ genes was also detected following the vaccinations, thus indicating that an adaptive immune response was induced by the vaccinations. Early increases in the expression levels of IL and IFN genes were also observed in the vaccinated fi sh. At four wpv, the fi sh were challenged with F . column a re , and the vaccinated fi sh showed a good level of protection against this pathogen, with 39% relative percent survival (RPS) compared with the control group. It can be concluded, therefore, that the fi ve OMPs, in the form of a recombinant fusion protein vaccine, induced an immune response in fi sh and protection against F . columnare .

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Exogenous antigens and the stimulation of MHC class I restricted cell-mediated cytotoxicity: possible strategies for fish vaccines

Cited by 29 publications

References 106 publications

Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss)—how can environmental influences be detected?

Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss)—how can environmental influences be detected?

Preliminary approach to find synthetic peptides from nodavirus capsid potentially protective against sea bass viral encephalopathy and retinopathy

Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella

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