Exogenous expression of barley HvWRKY6 in wheat improves broad-spectrum resistance to leaf rust, Fusarium crown rot, and sharp eyespot

Li, Mengyu; Zhao, Shuqing; Yang, Jian; Ren, Yan; Su, Jun; Zhao, Jianjun; Ren, Xiaopeng; Wang, Chuyuan; Chen, Shisheng; Yu, Xiaoyan; Chen, Feng; Wang, Xiaodong

doi:10.1016/j.ijbiomac.2022.07.138

Cited by 6 publications

(3 citation statements)

References 49 publications

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“…For example, SlWRKY75 enhances tomato defenses against Pst DC3000 by activating the expression of SlGH3.3 [55]. The exogenous expression of barley HvWRKY6 enhances resistance to leaf rust, Fusarium crown rot, and sharp eyespot in wheat [56]. In Arabidopsis, AtWRKY50 specifically binds to LS10 region of the PR1 promoter and interacts with TGAs to synergistically activate PR1 expression [57].…”

Section: Discussionmentioning

confidence: 99%

A comprehensive analysis of the WRKY family in soybean and functional analysis of GmWRKY164-GmGSL7c in resistance to soybean mosaic virus

Zhao,

Wang,

et al. 2024

BMC Genomics

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Background Soybean mosaic disease caused by soybean mosaic virus (SMV) is one of the most devastating and widespread diseases in soybean producing areas worldwide. The WRKY transcription factors (TFs) are widely involved in plant development and stress responses. However, the roles of the GmWRKY TFs in resistance to SMV are largely unclear. Results Here, 185 GmWRKYs were characterized in soybean (Glycine max), among which 60 GmWRKY genes were differentially expressed during SMV infection according to the transcriptome data. The transcriptome data and RT-qPCR results showed that the expression of GmWRKY164 decreased after imidazole treatment and had higher expression levels in the incompatible combination between soybean cultivar variety Jidou 7 and SMV strain N3. Remarkably, the silencing of GmWRKY164 reduced callose deposition and enhanced virus spread during SMV infection. In addition, the transcript levels of the GmGSL7c were dramatically lower upon the silencing of GmWRKY164. Furthermore, EMSA and ChIP-qPCR revealed that GmWRKY164 can directly bind to the promoter of GmGSL7c, which contains the W-box element. Conclusion Our findings suggest that GmWRKY164 plays a positive role in resistance to SMV infection by regulating the expression of GmGSL7c, resulting in the deposition of callose and the inhibition of viral movement, which provides guidance for future studies in understanding virus-resistance mechanisms in soybean.

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Section: Discussionmentioning

confidence: 99%

A comprehensive analysis of the WRKY family in soybean and functional analysis of GmWRKY164-GmGSL7c in resistance to soybean mosaic virus

Zhao,

Wang,

et al. 2024

BMC Genomics

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“…HvWRKY6-OE have shown enhanced resistance to P. tritici pathotype THTT, Puccinia striiformis f. sp. tritici pathotype CYR32, Fusarium crown rot, and sharp eyespot to varying degrees (Li et al, 2020;Li et al, 2022). Transcriptome sequencing analysis showed that HvWRKY6 gene activated wheat defense response in a pathogen-independent manner.…”

Section: Sar-like Responses In Triticeae Crops Of Wheat and Barleymentioning

confidence: 99%

“…Transcriptome sequencing analysis showed that HvWRKY6 gene activated wheat defense response in a pathogen-independent manner. HvWRKY6 partially activates the SAR-associated transcripts, including calcium-associated disease resistance pathways and part of the effector-triggered immunity (ETI) (Li et al, 2022). HvWRKY6-mediated resistance response is related to the activation of SA pathway and the inhibition of ABA and JA pathways (Li et al, 2022).…”

Section: Sar-like Responses In Triticeae Crops Of Wheat and Barleymentioning

confidence: 99%

Enhancement of broad-spectrum disease resistance in wheat through key genes involved in systemic acquired resistance

Zhao,

Li,

Ren

et al. 2024

Front. Plant Sci.

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Systemic acquired resistance (SAR) is an inducible disease resistance phenomenon in plant species, providing plants with broad-spectrum resistance to secondary pathogen infections beyond the initial infection site. In Arabidopsis, SAR can be triggered by direct pathogen infection or treatment with the phytohormone salicylic acid (SA), as well as its analogues 2,6-dichloroisonicotinic acid (INA) and benzothiadiazole (BTH). The SA receptor non-expressor of pathogenesis-related protein gene 1 (NPR1) protein serves as a key regulator in controlling SAR signaling transduction. Similarly, in common wheat (Triticum aestivum), pathogen infection or treatment with the SA analogue BTH can induce broad-spectrum resistance to powdery mildew, leaf rust, Fusarium head blight, and other diseases. However, unlike SAR in the model plant Arabidopsis or rice, SAR-like responses in wheat exhibit unique features and regulatory pathways. The acquired resistance (AR) induced by the model pathogen Pseudomonas syringae pv. tomato strain DC3000 is regulated by NPR1, but its effects are limited to the adjacent region of the same leaf and not systemic. On the other hand, the systemic immunity (SI) triggered by Xanthomonas translucens pv. cerealis (Xtc) or Pseudomonas syringae pv. japonica (Psj) is not controlled by NPR1 or SA, but rather closely associated with jasmonate (JA), abscisic acid (ABA), and several transcription factors. Furthermore, the BTH-induced resistance (BIR) partially depends on NPR1 activation, leading to a broader and stronger plant defense response. This paper provides a systematic review of the research progress on SAR in wheat, emphasizes the key regulatory role of NPR1 in wheat SAR, and summarizes the potential of pathogenesis-related protein (PR) genes in genetically modifying wheat to enhance broad-spectrum disease resistance. This review lays an important foundation for further analyzing the molecular mechanism of SAR and genetically improving broad-spectrum disease resistance in wheat.

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Determination of resistance levels of Hordeum vulgare L. cultivars grown in Türkiye against Fusarium culmorum

Yörük,

Ünal,

Özsoy

et al. 2024

CEREAL RESEARCH COMMUNICATIONS

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Exogenous expression of barley HvWRKY6 in wheat improves broad-spectrum resistance to leaf rust, Fusarium crown rot, and sharp eyespot

Cited by 6 publications

References 49 publications

A comprehensive analysis of the WRKY family in soybean and functional analysis of GmWRKY164-GmGSL7c in resistance to soybean mosaic virus

A comprehensive analysis of the WRKY family in soybean and functional analysis of GmWRKY164-GmGSL7c in resistance to soybean mosaic virus

Enhancement of broad-spectrum disease resistance in wheat through key genes involved in systemic acquired resistance

Determination of resistance levels of Hordeum vulgare L. cultivars grown in Türkiye against Fusarium culmorum

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