Exogenous Inoculation of Microorganisms Effect on Root Exudates and Rhizosphere Microorganism of Tobaccos

Guo, Qingqing; Yu, Jae Hyeon; Sun, Jingguo; Wang, Changjun; Chen, Shouwen; Yang, Chunlei; Yang, Yong

doi:10.4236/aim.2021.119038

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Perhaps because of the different extraction methods, detection methods, and plant species, we did not detect large amounts of sugars, fatty acids, amino acids, and other substances, in contrast to the results of previous studies [60,61]. Related methods need to be continuously optimized in follow-up research to more accurately determine the composition of root exudates [62]. The relationships between root exudates and rhizosphere soil microorganisms also require further study [60].…”

Section: Discussioncontrasting

confidence: 55%

Effects of Trichoderma asperellum 6S-2 on Apple Tree Growth and Replanted Soil Microbial Environment

Wang

Zhang

Mao

et al. 2022

JoF

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Trichoderma asperellum strain 6S-2 with biocontrol effects and potential growth-promoting properties was made into a fungal fertilizer for the prevention of apple replant disease (ARD). 6S-2 fertilizer not only promoted the growth of Malus hupehensis Rehd seedlings in greenhouse and pot experiments, but also increased the branch elongation growth of young apple trees. The soil microbial community structure changed significantly after the application of 6S-2 fertilizer: the relative abundance of Trichoderma increased significantly, the relative abundance of Fusarium (especially the gene copy numbers of four Fusarium species) and Cryptococcus decreased, and the relative abundance of Bacillus and Streptomyces increased. The bacteria/fungi and soil enzyme activities increased significantly after the application of 6S-2 fertilizer. The relative contents of alkenes, ethyl ethers, and citrullines increased in root exudates of M. hupehensis Rehd treated with 6S-2 fertilizer and were positively correlated with the abundance of Trichoderma. The relative contents of aldehydes, nitriles, and naphthalenes decreased, and they were positively correlated with the relative abundance of Fusarium. In addition, levels of ammonium nitrogen (NH4-N), nitrate nitrogen (NO3-N), available phosphorus (AP), available potassium (AK), organic matter (SOM), and pH in rhizosphere soil were also significantly related to changes in the microbial community structure. In summary, the application of 6S-2 fertilizer was effective in alleviating some aspects of ARD by promoting plant growth and optimizing the soil microbial community structure.

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Section: Discussioncontrasting

confidence: 55%

Effects of Trichoderma asperellum 6S-2 on Apple Tree Growth and Replanted Soil Microbial Environment

Wang

Zhang

Mao

et al. 2022

JoF

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“…Otherwise, sclareolide was quite abundant in the samples of South America and the cigar samples of East Asia were rich in hexadecanoic acid, methyl ester. Meanwhile, the microbial could produce volatile metabolites by acting on tobacco leaves during the fermentation (Yan et al, 2020;Guo et al, 2021;Shimasaki et al, 2021). In this work we noticed that the Actinobacteria was positively correlated with aldehydes and ketones (Zheng et al, 2022b), which will help subsequent studies in improving tobacco quality.…”

Section: Integrating Volatile Metabolites and Microbial Of Cigars Fro...mentioning

confidence: 72%

Phyllosphere microbial community of cigar tobacco and its corresponding metabolites

Liu

Guo

et al. 2022

Front. Microbiol.

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Cigar is made of a typical fermented tobacco where the microbiota inhabits within an alkaline environment. Our current understanding on cigar fermentation is far from thorough. This work employed both high-throughput sequencing and chromatography-mass spectrometric technologies to provide new scientific reference for this specific fermented system. Typical cigar samples from different regions (the Caribbeans, South America, East Asia, and Southeast Asia) were investigated. The results show that Firmicutes, Actinobacteria, Proteobacteria, Ascomycota, and Basidiomycota were the predominant phyla in the cigar samples. Rather than the fungal community, it was the bacterial community structures that played vital roles to differentiate the cigar from different regions: Staphylococcus was the dominant genus in the Americas; Bacillus was the dominant genus in Southeast Asia; while in East Asia, there was no dominant genus. Such differences in community structure then affected the microflora metabolism. The correlation between microbiota and metabolites revealed that Aspergillaceae, Cercospora, and Staphylococcus were significantly correlated with sclareolide; Bacillus were positively associated with isophorone. Alcaligenaceae was significantly and positively correlated with L-nicotine and hexadecanoic acid, methyl ester.GRAPHICAL ABSTRACT

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“…The input of plant-derived lipids to soils includes three pathways, i.e., litter fall, root exudates, and rhizodeposition (Upadhayay et al, 2020;Dai et al, 2022). The major lipid components of tobacco root exudates are myristic acid, lauric acid, benzoic acid, palmitic acid, cinnamic acid, stearic acid, benzoic acid, and phenylpropionic acid (Li et al, 2017;Banozǐcé t al., 2021;Guo et al, 2021). Animal-derived lipids are mainly the decomposition products of animal carcasses after death (Durães et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Exogenous myristate promotes the colonization of arbuscular mycorrhizal fungi in tomato

Liu,

Feng,

Zhang

et al. 2023

Front. Plant Sci.

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Arbuscular mycorrhizal fungi (AMF) can establish symbiotic associations with the roots of most terrestrial plants, thereby improving the tolerance of the host plants to biotic and abiotic stresses. Although AMF cannot synthesize lipids de novo, they can obtain lipids from the root cells for their growth and development. A recent study reveals that AMF can directly take up myristate (C14:0 lipid) from the environment and produce a large amount of hyphae in asymbiotic status; however, the effect of environmental lipids on AM symbiosis is still unclear. In this study, we inoculated tomato (Solanum lycopersicum) with AMF in an in vitro dual culture system and a sand culture system, and then applied exogenous myristate to the substrate, in order to explore the effect of exogenous lipids on the mycorrhizal colonization of AMF. We investigated the hyphae growth, development, and colonization of AMF, and examined the gene expression involved in phosphate transport, lipid biosynthesis, and transport. Results indicate that exogenous lipids significantly stimulated the growth and branching of hyphae, and significantly increased the number of hyphopodia and mycorrhizal colonization of AMF, with arbuscular abundance and intraradical spores or vesicles being the most promoted. In contrast, exogenous myristate decreased the growth range and host tropism of the germ tubes, and largely inhibited the exchange of nutrition between symbionts. As a result, exogenous myristate did not affect the plant growth. This study suggests that lipids promote mycorrhizal colonization by enhancing the growth and development of AMF hyphae and increasing their contact opportunities with plant roots. To the best of our knowledge, this is the first report that shows that lipids promote the colonization of AMF. Our study highlights the importance of better understanding the roles of environmental lipids in the establishment and maintenance of AM symbiosis and, thus, in agricultural production.

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Exogenous Inoculation of Microorganisms Effect on Root Exudates and Rhizosphere Microorganism of Tobaccos

Cited by 6 publications

References 25 publications

Effects of Trichoderma asperellum 6S-2 on Apple Tree Growth and Replanted Soil Microbial Environment

Effects of Trichoderma asperellum 6S-2 on Apple Tree Growth and Replanted Soil Microbial Environment

Phyllosphere microbial community of cigar tobacco and its corresponding metabolites

Exogenous myristate promotes the colonization of arbuscular mycorrhizal fungi in tomato

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