Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean

Abstract

Understanding the relationship between exogenous melatonin and water deficit stress is crucial for achieving high yields and alleviating the effects of water deficit stress on soybean (Glycine max (L.) Merrill) plants in agriculture. This study investigated the effects of exogenous melatonin on soybean photosynthetic capacity under water deficit stress induced by polyethylene glycol (PEG) 6000. We conducted a potting experiment in 2018 using the soybean (Glycine max L. Merrill) cultivar Suinong 26. We identified the impacts of a concentration of PEG 6000 simulating drought (15%, w/v) and an appropriate melatonin concentration (100 μmol/L) on the growth of soybean seedlings and flowering stages in a preliminary test. We applied exogenous melatonin by foliar spraying and root application to determine the effects on leaf photosynthesis during water deficit stress. Our results indicated that 15% PEG 6000 had an obvious inhibitory effect on the growth of soybean seedlings and flowering stages, causing oxidative stress and damage due to reactive oxygen species (ROS) (H2O2 and O2·-) accumulation and potentially reducing air exchange parameters and photosystem II (PSII) efficiency. The application of exogenous melatonin significantly relieved the inhibitory effects of PEG 6000 stress on seedlings and flowering growth, and gas exchange parameters, potentially improved PSII efficiency, improved the leaf area index (LAI) and the accumulation of dry matter, slowed down oxidative stress and damage to leaves by increasing the activity of antioxidant enzymes (SOD, POD, and CAT), reduced the content of malondialdehyde (MDA), and ultimately improved soybean yield. Overall, the results of this study demonstrated that application of exogenous melatonin at the seedlings and flowering stages of soybean is effective in alleviating plant damage caused by water deficit stress and improving the drought resistance of soybean plants. In addition, the results showed that application of exogenous melatonin by root is superior to foliar spraying.

Melatonin Regulates Chloroplast Protein Quality Control via a Mitogen-Activated Protein Kinase Signaling Pathway

“…In addition to the genetic evidence, exogenous melatonin treatment conferred stress tolerance to a wide range of biotic and abiotic stressors, including viral attack [ 8 , 54 , 55 ]. In addition to being involved in defense responses, melatonin is also involved in plant growth in conjunction with photosynthesis when plants were challenged with abiotic stresses [ 20 , 56 ]. For example, melatonin treatment enhanced soybean growth by increasing PSI- and PSII-related genes and increased the rate of photosynthesis in Chinese hickory and grape seedlings upon stresses [ 57 , 58 , 59 ].…”

Melatonin-Induced Water Stress Tolerance in Plants: Recent Advances

“…In this respect, melatonin enhances plant resistance via higher stomatal conductance [ 42 ], higher cell turgor and water holding capacity [ 65 ], and stomatal opening regulation [ 77 ]. Consequently, the whole plant status is enhanced, including seed germination efficiency [ 47 ], root generation vitality and strength [ 61 ], growth and flowering [ 36 , 50 ], visual quality [ 65 ], seed yield [ 38 ], leaf senescence alleviation [ 54 ], and quick recovery after rehydration [ 59 ].…”

Exogenous salicylic acid ameliorates waterlogging stress damages and improves photosynthetic efficiency and antioxidative defense system in waxy corn

“…Photosynthesis is the key process necessary for the growth and development of higher plants; it is also one of the processes most sensitive to waterlogging stress (Sterling et al 2019, Zhang et al 2019b. The preliminary experiments in this study revealed that waterlogging stress reduced PN, E, gs, and Ci, and PN decreased more rapidly in SY-XT1 than that in SY-MY13 ( Fig.…”