Exome Sequencing and Advances in Crop Improvement

Plant exomics: Concepts, applications and methodologies in crop improvement

“…20 These Ultra-high sequencing platforms that omit Sanger's Chain termination sequencing technology are classified as next or second generation sequencing platforms. 6 These methodologies are based on diverse chemistries and detection approaches. While some of the techniques involved share very few features, key characteristic is common to all.…”

“…6 New sequencing techniques are thus formulated and commercialized recently to provide users novel approaches that can be utilized in exome sequencing to study physical traits, molecular markers identification, larger exome library formulation and unlocking Mendelian disease. 20 These Ultra-high sequencing platforms that omit Sanger's Chain termination sequencing technology are classified as next or second generation sequencing platforms.…”

“…Luciferase at the end causes the release of luminance by converting luciferin into oxyluciferin and its intensity is relative to the number of nucleotides added in the DNA polymerase. 6 The agarose beads used in the process have oligonucleotides on their surfaces, corresponding to the 454-exclusive adaptors on the library of DNA fragments. 22 This causes the association of a single bead with a single fragment and these complexes are then separated and added to micelles.…”

“…Each DNB consists of repetitive copies of the original circles and is approximately 200 nm in diameter. 6 The DNBs are then adhered to a silicon chip. This approach of unchained ligation sequencing employs anchor probes (locate the adaptor sites) and probes (used for detection and consist of nanomeres along with fluorescent dye).…”

“…These sequences representing about a hundredth of whole genome includes all the sequences that code for proteins, which are responsible for phenotypic regulation although they do not give a complete picture of gene regulation. 6 Exome Sequencing has emerged as a vital genetic tool for revealing molecular basis of diseases and phenotypic traits.…”